34,218 results
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Plasmid#122017PurposeExpresses genes for lycopene synthesis (crtI, crtB & ipi from Pantoea agglomerans, ggpps from T. canadiensis). IPTG inducible. Spec. resist.DepositorInsertGGPP synthase
ExpressionBacterialMutationcodon optimized, truncated first 98 amino acids, …PromoterT7Available sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150)
Plasmid#159689PurposeE. coli protein expression of His6-MBP-tev-G-Hs.PDE6D(2-150)DepositorAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL5-pCMV7.1
Plasmid#155024PurposeN-terminal 3xFLAG-tagged CUL5 in a pCMV7.1 vectorDepositorAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAT03-Apr
Plasmid#154756PurposeFor curing of the resistance marker of RecET recombinase system in Acinetobacter baumannii, AprarDepositorInsertFLP recombinase
ExpressionBacterialAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-1-mRhubarb720-HO
Plasmid#141201PurposeExpresses mRhubarb720 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb720
heme oxygenase
Tags6x-HisExpressionBacterialMutationR134H, L196Q, T202D, V203I, W309R, Q310A compared…PromoterT7Available sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCK005.6
Plasmid#153025PurposedCas9, MCP-SoxS(R93A/S101A), J106 scRNADepositorInsertdCas9, MCP-SoxS(R93A/S101A), J106 scRNA
UseCRISPRExpressionBacterialMutationSoxS has R93A and S101A mutationsAvailable sinceAug. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
P22_wtCP_SP141his
Plasmid#122651PurposeExpression of P22 virus like particle in bacteriaDepositorInsertP22 Coat: WT / SP: 6xHis-SP141
ExpressionBacterialMutationtruncated SP, P120H in the capsid scaffold (see …Available sinceAug. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS sfGFP
Plasmid#154462PurposepET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available sinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET19b+H3
Plasmid#157807PurposeExpression of cysteine-free human histone H3 (c111A)DepositorInsertH3
ExpressionBacterialMutationC111APromoterT7Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#15
Plasmid#153523PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#15DepositorPublicationInsertSb#15
ExpressionBacterialPromoterpBADAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1.H1-NP
Plasmid#134367PurposeNanoluc complementation assay. Expression of histamine receptor H1 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of the Flag epitope at N terminus of H1 receptor.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH1-NP (HRH1 Human)
TagsFlag and natural peptide of nanoluciferaseExpressionBacterial and MammalianPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS TIR-1 sfGFP
Plasmid#154463PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDonor-GDH
Plasmid#140629PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.DepositorInsertMini-Tn6677, T7lac-GDH gene cassette
UseCRISPR; TransposonExpressionBacterialAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGBW-m4092694
Plasmid#153907PurposeBacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stopDepositorInsertfragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionBacterialMutationwtPromoterPromoter | t7 consensus from NEB, G is +1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSC101-ΔPS-PM,CS-RBSBBa_B0034-geneVI
Plasmid#134356PurposeAccessory plasmidDepositorInsertGene VI
ExpressionBacterialAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMAK461
Plasmid#140699PurposeNBαRb-IgG-FcDepositorInsertNBαRb-IgG-Fc
ExpressionBacterialAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-6His-AcpS
Plasmid#145376PurposeExpression of E. coli AcpSDepositorAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4136458
Plasmid#149531PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 13, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBWB419
Plasmid#140637PurposeRSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.DepositorInsertCas9
ExpressionBacterialAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046747
Plasmid#149249PurposeBacterial expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits