31,059 results
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Plasmid#18097DepositorAvailable SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only
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PGEX-NEDD4
Plasmid#45043DepositorAvailable SinceJune 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTlpA_mCherry
Plasmid#229146PurposePromoter strength evaluation of PtlpADepositorInsertmCherry
ExpressionBacterialMutationNoPromoterPtlpAAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSG4K5
Plasmid#74492PurposeFor cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pgRNA
Plasmid#215560PurposeConstitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanRDepositorInsertgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSR78
Plasmid#90210PurposeExpresses the light sensors CcaS and Cph8 as well as the phycocyanobilin biosynthesis genes Ho1 and PcyADepositorInsertsCcaS
Cph8
Ho1
PcyA
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_hph
Plasmid#184915PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_mic
Plasmid#184916PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-hAos1
Plasmid#53135PurposeE.coli expression of full length human Aos1 (SAE1) with Nterminal His-tagDepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_P0031-mCherryBb
Plasmid#118240PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0031 promoter driven mCherryDepositorInsertbb0031 promoter driven mCherry
ExpressionBacterialAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSR0
Plasmid#161984PurposeAllele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection markerDepositorTypeEmpty backboneExpressionBacterialAvailable SinceOct. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
p15TV-L
Plasmid#26093PurposeSGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.DepositorTypeEmpty backboneTags6xHis and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceSept. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJUMP49-2E(sfGFP)
Plasmid#126995PurposeLevel 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Bcatfl
Plasmid#24193DepositorAvailable SinceMarch 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJS61
Plasmid#207585PurposeGFP-TolA expressionDepositorInsertGFP-TolA
ExpressionBacterialAvailable SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (1-204) (pGEX2T(TEV))
Plasmid#80632PurposeResidues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable GST tag; Sundquist Lab internal ID: WISP06-198DepositorAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Venus-pBAD
Plasmid#54859PurposeLocalization: Protein Expression Vector , Excitation: 515, Emission: 528DepositorTypeEmpty backboneTags6xHis and VenusExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET51b-His-TEV-SNAP-tag_fast
Plasmid#167271PurposeOverexpression of the fast variant (E30R) of SNAP-tag in E. coliDepositorInsertSNAPf-tag
TagsHis x10ExpressionBacterialMutationE30RPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA
Plasmid#119946PurposeExpresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorInsertRenilla luciferase
Tags24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …ExpressionBacterial and MammalianPromoterTetCMVAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMGE-T7
Plasmid#153021PurposeSingle-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.DepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only