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strep-IRF8

Plasmid

#102872

Purpose

Express strep-tagged mouse IRF8 protein in E. coli

Depositor

Gary Stormo

Publication

Chang et al BMC Mol Biol. 2018 Mar 27;19(1):5. doi:

Insert

IRF8 (Irf8 Mouse)

Tags

strep tag

Expression

Bacterial

Promoter

T7

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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zCD2

Plasmid

#122031

Purpose

Expresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.

Depositor

David Christianson

Publication

Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

Insert

Zebrafish HDAC6 Catalytic Domain 2

Tags

His6-MBP tag with cleavable TEV site

Expression

Bacterial

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

Plasmid

#122085

Purpose

Express M.EcoGII fused to CENPC with destabilization domain - inducible

Depositor

Laure Crabbe

Publication

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII-V5-CENPC (CENPC Human, E.coli)

Use

Retroviral

Tags

V5 tag on Nter of CENPC

Expression

Bacterial and Mammalian

Mutation

L341F, R930L (see comments below)

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDFDuet-MKK6-EE

Plasmid

#82718

Purpose

Expresses constitutively active human MKK6. Multiple cloning site 2 is empty.

Depositor

Kevin Janes

Publication

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK6 (MAP2K6 Human)

Expression

Bacterial

Mutation

Ser207 to Glu, Thr211 to Glu

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGEX-4T-1 3xFlag-STAT1

Plasmid

#82722

Purpose

Expresses human STAT1

Depositor

Kevin Janes

Publication

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

STAT1 (STAT1 Human)

Tags

3xFLAG and GST

Expression

Bacterial

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pMal-Abl-PRM 5R

Plasmid

#112088

Purpose

Bacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.

Depositor

Michael Rosen

Publication

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

PRM-5R (ABL1 Human)

Tags

His-6 and MBP

Expression

Bacterial

Mutation

construct contains only the PRM motif of Abl1

Promoter

Lac

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDD87

Plasmid

#119892

Purpose

The pLPD03031 promoter from Rhodococcus opacus PD630 expressing GFP+ on the pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Publication

DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

Insert

pLPD03031_GFP+

Expression

Bacterial

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTW084b

Plasmid

#116894

Purpose

Activity-independent SE-PACE AP

Depositor

David Liu

Publication

Wang et al Nat Chem Biol. 2018 Aug 20. pii: 10.1038

Inserts

g3N
434-cI/scFv m3

Expression

Bacterial

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBSV2H

Plasmid

#118229

Purpose

Empty E. coli - B. burgdorferi shuttle vector; hygromycin B resistant

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Expression

Bacterial

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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6BHD (SETDB1)

Plasmid

#119955

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Insert

SETDB1 (SETDB1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

contains amino acids 190-410

Promoter

T7

Available since

Feb. 1, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pBAD/HisD-moxMaple3

Plasmid

#120871

Purpose

Bacterial expression of moxMaple3

Depositor

Vladislav Verkhusha

Publication

Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

Insert

moxMaple3

Tags

His

Expression

Bacterial

Mutation

from mMaple3, moxMaple3 was engineered by replaci…

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pdCas9-J23109

Plasmid

#113151

Purpose

Plasmid constitutively expressing dCas9 protein

Depositor

Chong Zhang

Publication

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

dCas9

Expression

Bacterial

Mutation

D10A & H840A

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1_RAF1 (51-131)

Plasmid

#119218

Purpose

purification of GST-RAF1 RBD from E. coli

Depositor

Helen Morrison

Publication

Riecken et al Hum Mutat. 2015 Feb;36(2):270-8. doi: 10

Insert

RAF1 aa 51-131 (RAF1 Human)

Expression

Bacterial

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTn7xKS

Plasmid

#117393

Purpose

Tn7 tagging vector with a LacI-controlled kill switch counterselection system

Depositor

Karen Guillemin

Publication

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE143

Plasmid

#118495

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Expression

Bacterial

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-130

Plasmid

#118548

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE162

Plasmid

#118510

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Expression

Bacterial

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-131

Plasmid

#118549

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-156

Plasmid

#118570

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLBH546_Tet-Cas14b1Locus

Plasmid

#112503

Purpose

Expresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Publication

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14b1

Use

CRISPR

Expression

Bacterial

Promoter

Tet

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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