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31,059 results

Showing: 11751 - 11800 of 31059 results

  4. pGBW-m4046921

    Plasmid
    #145755
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145755-plasmid-map-sequence-id-292520

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  6. pCHAP4

    Plasmid
    #206850
    Purpose
    A plasmid containing a 16,121 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector.
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Available Since
    Feb. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    206850-plasmid-map-sequence-id-420756

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  9. pAV11K

    Plasmid
    #63184
    Purpose
    pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63184-plasmid-map-sequence-id-104886

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  13. PX459-gR2A_Hinge

    Plasmid
    #124811
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Cas9 – gRNA_R2A_hinge
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Bacterial and Mammalian
    Mutation
    R166H in PuroR (please see depositors comment bel…
    Promoter
    pUC
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124811-plasmid-map-sequence-id-243700

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  15. pDB026a

    Plasmid
    #109260
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp_pIII(P29*); luxAB; PproK_TyrT(Opt, CUA);
    Expression
    Bacterial
    Mutation
    amber codon at position 29 gIII
    Promoter
    Ppsp; PproK
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    109260-plasmid-map-sequence-id-210477

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  19. pGBW-m4046865

    Plasmid
    #153930
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153930-plasmid-map-sequence-id-307315

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  21. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79887
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Expression
    Bacterial
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79887-plasmid-map-sequence-id-402553

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  31. pLIC-SGC1

    Plasmid
    #39187
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Nicola Burgess-Brown
    Type
    Empty backbone
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39187-plasmid-map-sequence-id-160533

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  32. pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)

    Plasmid
    #153442
    Purpose
    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Tags
    3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    153442-plasmid-map-sequence-id-310821

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  35. pET28a-ybbr-HIS-ddFLN4-K10

    Plasmid
    #101718
    Purpose
    K10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    ddFLN4 fingerprint, K10 peptide (KRT10 Dictyostelium discoideum, Human)
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101718-plasmid-map-sequence-id-196776

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  46. pBAD_mCherry_GFP_++

    Plasmid
    #187389
    Purpose
    arabinose-inducible expression of GFP and mCherry
    Depositor
    Roi Avraham
    Insert
    GFP, mCherry
    Expression
    Bacterial
    Available Since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187389-plasmid-map-sequence-id-371118

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  48. mOrange in pBSDONR P4r-P2

    Plasmid
    #121529
    Purpose
    For making C-terminal mOrange fusions using multisite Gateway cloning
    Depositor
    Roger Innes
    Insert
    mOrange
    Expression
    Bacterial
    Available Since
    April 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    121529-plasmid-map-sequence-id-234042

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Showing: 11751 - 11800 of 31059 results