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pMCSG53_N

Plasmid

#167255

Purpose

Bacterial expression of Nucleocapsid protein (residues 1-419)

Depositor

Alexei Savchenko

Insert

N protein (residues 1-419) (N SARS-CoV-2)

Tags

His6-TEV site

Expression

Bacterial

Mutation

none

Available since

April 27, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET51b-His-TEV-SNAP-tag_fast

Plasmid

#167271

Purpose

Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Publication

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAPf-tag

Tags

His x10

Expression

Bacterial

Mutation

E30R

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET51b-His-TEV-CLIP-tag

Plasmid

#167274

Purpose

Overexpression of CLIP-tag in E. coli

Depositor

Kai Johnsson

Publication

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

CLIP-tag

Tags

His x10

Expression

Bacterial

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-CRM197-4xDQNAT

Plasmid

#128403

Purpose

Periplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Publication

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Diphtheria toxin

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Mutation

Inactivating G52E mutation

Promoter

trc

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1385

Plasmid

#166862

Purpose

Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

Depositor

Irina Artsimovitch

Publication

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pQE-30-RexYFP

Plasmid

#141070

Purpose

Bacterial expression of genetically encoded fluorescent indicator for measuring NAD+/NADH ratio

Depositor

Vsevolod Belousov

Publication

Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

Insert

RexYFP

Tags

6xHis

Expression

Bacterial

Mutation

changed Leucine 169 to Proline, changed Tyrosine …

Promoter

T5

Available since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a.Tat(48-57)-EGFP

Plasmid

#165040

Purpose

Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

Depositor

Dokyun Na

Publication

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

EGFP

Tags

6XHis and Tat(48-57)

Expression

Bacterial

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pNHEJ-recAmu-sacB

Plasmid

#158717

Purpose

CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.

Depositor

Yi-Cheng Sun

Publication

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

recA

Expression

Bacterial

Mutation

R61C

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBWB294

Plasmid

#140636

Purpose

pBAV1k-lacI-Trc-BCD9-mCherry. BCD RBS.

Depositor

Keith Tyo

Publication

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

mCherry

Expression

Bacterial

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNHEJ-Cas12a-recX

Plasmid

#158714

Purpose

CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recX

Depositor

Yi-Cheng Sun

Publication

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

recX

Use

CRISPR

Expression

Bacterial

Promoter

Pmyc

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-RT007-GCN4scFV-VB

Plasmid

#163387

Purpose

2nd generation lentiviral vector to express anti-GCN4 module in the G-baToN system

Depositor

Monte Winslow

Publication

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

antiGCN4scFV-VEEGFRtm-BFP (GCN4scFV-VB)

Use

Lentiviral

Tags

BFP

Expression

Bacterial and Mammalian

Promoter

EF1a

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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nuc-mCherry-pTRIP

Plasmid

#163520

Purpose

Used to construct lentivirus to express nucleus-targeted mCherry in mammalian cells

Depositor

Thomas Weber

Insert

nucleus targeted mCherry

Use

Lentiviral

Expression

Bacterial and Mammalian

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNHEJ-Cas12a

Plasmid

#158713

Purpose

CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum

Depositor

Yi-Cheng Sun

Publication

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

the MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)

Use

CRISPR

Expression

Bacterial

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNA500

Plasmid

#149576

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLG016 (ApeA)

Plasmid

#157894

Purpose

Expresses the ApeA defense system

Depositor

Feng Zhang

Publication

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

ApeA (putative HEPN RNase)

Expression

Bacterial

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTXB1-TP3

Plasmid

#160086

Purpose

Bacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKA

Depositor

Pier Pandolfi

Publication

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase/APEX2 peroxidase fusion protein

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_DNASE1_Exo-endo-phos

Plasmid

#110004

Purpose

Protein expression and purification of DNASE1_Exo-endo-phos

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DNASE1_Exo-endo-phos (DNASE1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAG_14-3-3

Plasmid

#109905

Purpose

Protein expression and purification of YWHAG_14-3-3

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAG_14-3-3 (YWHAG Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Dual opposing T7 promoters reporter plasmid

Plasmid

#156457

Purpose

A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

Depositor

Matthew D. Shoulders

Publication

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Insert

NeoR/KanR

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

Em7

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)

Plasmid

#156463

Purpose

Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM

Depositor

Andre Stiel

Publication

Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

Inserts

Heme Oxygenase
ReBphP-PCM

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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