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pGEX-6P-1-INTS3-FL

Plasmid

#128415

Purpose

Express INTS3 full-length in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Tags

GST

Expression

Bacterial

Mutation

full-length

Promoter

tac promoter

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-L1T1L-NLuc

Plasmid

#128942

Purpose

Expresses L1-NLuc gene

Depositor

Takeshi Kobayashi

Article

Kanai et al J Virol. 2019 May 8. pii: JVI.00401-19.

Insert

L1 minor core protein

Tags

Nanoluc

Expression

Bacterial

Promoter

T7 promotor

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1

Plasmid

#128306

Purpose

Express hNABP1 (a.k.a hSSB2 or SOSSB2) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human nucleic acid-binding protein 1 (hNABP1) (NABP1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pBT13-splitC

Plasmid

#122596

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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OmpA-mNG-mCh

Plasmid

#124216

Purpose

Periplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mCh

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al MBio. 2017 Sep 12;8(5). pii: mBio.01089-

Insert

OmpA-mNG-mCh

Expression

Bacterial

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-Ah7

Plasmid

#122574

Purpose

MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

catP/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-Ah10

Plasmid

#122577

Purpose

MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

tetQ/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-Ah3

Plasmid

#122570

Purpose

MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pET41a iGluu

Plasmid

#119834

Purpose

Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluu

Tags

GST

Expression

Bacterial

Mutation

S72T

Promoter

T7

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAX1

Plasmid

#117397

Purpose

allelic exchange vector with GFP merodiploid tracker and temperature-sensitive origin of replication

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD011

Plasmid

#116852

Purpose

Expresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
mScarletI

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-3R

Plasmid

#112091

Purpose

Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-3R (NCK1 Human)

Tags

GST

Expression

Bacterial

Promoter

Lac

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-His tagged nuclear Pif1

Plasmid

#65047

Purpose

expression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tag

Depositor

Jean-Baptiste Boulé, Virgina Zakian

Article

Boule et al Nature. 2005 Nov 3;438(7064):57-61. Epub

Insert

Yeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)

Tags

histag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-4R

Plasmid

#112090

Purpose

Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-4R (NCK1 Human)

Tags

GST

Expression

Bacterial

Promoter

Lac

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMal-Abl-PRM 5R

Plasmid

#112088

Purpose

Bacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

PRM-5R (ABL1 Human)

Tags

His-6 and MBP

Expression

Bacterial

Mutation

construct contains only the PRM motif of Abl1

Promoter

Lac

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTW084b

Plasmid

#116894

Purpose

Activity-independent SE-PACE AP

Depositor

David Liu

Article

Wang et al Nat Chem Biol. 2018 Aug 20. pii: 10.1038

Inserts

g3N
434-cI/scFv m3

Expression

Bacterial

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMM534

Plasmid

#112530

Purpose

pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Insert

luxCDABE

Expression

Bacterial

Promoter

PL(HrtO)

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-N-Strep2

Plasmid

#119758

Purpose

Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.

Depositor

Andrew Jackson, Martin Reijns

Type

Empty backbone

Tags

GST and Strep-tag II fusion tag

Expression

Bacterial

Promoter

tac promoter

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTHSSe_45

Plasmid

#109243

Purpose

pSC101 var3 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1358

Plasmid

#119277

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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