30,803 results

1. pTXB1.Tat(48-57)-Cre recombinase

   Plasmid

   #165042

   Purpose

   Expression of Tat(48-57) conjugated Cre recombinase for protein purification (Intein-chitin binding domain fussion)

   Depositor

   Dokyun Na

   Article

   Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

   Insert

   Cre recombinase
   

   Tags

   Mxe Intein-Chitin binding domain and Tat(48-57)

   Expression

   Bacterial

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMApra-Ptet1

   Plasmid

   #218360

   Purpose

   Empty backbone with tetR-PtetA expression system with single tetR operator site

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM41_KPNA4_Arm

   Plasmid

   #178478

   Purpose

   Bacterial expression of human domain with His-tag and MBP tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   KPNA4_Arm (KPNA4 Synthetic, Human)
   

   Tags

   His-MBP

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHYSTC299

   Plasmid

   #124354

   Purpose

   Plasmid expressing gp41-1 intein inserted into CcdA antitoxin

   Depositor

   Hideo Iwai

   Article

   Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

   Insert

   gp41-1 intein
   

   Tags

   CcdA-C (SFAD)

   Expression

   Bacterial

   Promoter

   STC

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GST-TAX1BP1

   Plasmid

   #171423

   Purpose

   For the purifcation of TAX1BP1 protein

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   TAX1BP1 (TAX1BP1 Human)
   

   Expression

   Bacterial

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. BglB in pET29b(+)

   Plasmid

   #55773

   Purpose

   Expresses beta-glucosidase

   Depositor

   Justin Siegel

   Article

   Carlin et al PLoS One. 2016 Jan 27;11(1):e0147596. do

   Insert

   Beta-glucosidase B (bglB Synthetic)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-TRBP

   Plasmid

   #50351

   Purpose

   bacterial expression of TRBP

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

   Insert

   TRBP (TARBP2 Human)
   

   Tags

   His and Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pST50Trc3-HISNDHFR

   Plasmid

   #63976

   Purpose

   Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   6xHis; TEV cleavable

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. p0857 pBR322 SV40

   Plasmid

   #10877

   Depositor

   Peter Howley

   Insert

   SV40
   

   Expression

   Bacterial

   Available Since

   Oct. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pEB1-wtGFP

    Plasmid

    #103978

    Purpose

    Plasmid encoding wtGFP

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    wtGFP
    

    Use

    Low copy

    Expression

    Bacterial

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. KAT2B

    Plasmid

    #39002

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KAT2B (KAT2B Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. FepA

    Plasmid

    #26039

    Depositor

    Johann Deisenhofer

    Article

    Buchanan et al Nat Struct Biol. 1999 Jan . 6(1):56-63.

    Insert

    FepA (fepA E Coli)
    

    Expression

    Bacterial

    Available Since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pRSETb-pcDronpa2-A69T

    Plasmid

    #98578

    Purpose

    photoconvertible fluorescent protein pcDronpa2-A69T in pRSetB plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

    Depositor

    Ulrike Endesfelder

    Article

    Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

    Insert

    pcDronpa2-A69T
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. HECTD1

    Plasmid

    #25183

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    hectd1.1273.1343 (HECTD1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 9, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pGEN6-Ubc5 Sc

    Plasmid

    #83234

    Purpose

    pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Inserts

    Ubiqutin (UBC Human)
    Ubc 5 (UBC5 Budding Yeast)
    Uba 1
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)

    Plasmid

    #117910

    Purpose

    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uM

    Depositor

    Kai Johnsson

    Article

    Yu et al Science. 2018 Sep 14;361(6407):1122-1126

    Insert

    SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    R44A on eDHFR

    Promoter

    T7

    Available Since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDSG360

    Plasmid

    #115601

    Purpose

    surface-displayed Ag3; medium copy

    Depositor

    Ingmar Riedel-Kruse

    Article

    Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

    Insert

    regulated adhesin construct
    

    Expression

    Bacterial

    Available Since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWZ403

    Plasmid

    #208113

    Purpose

    mSwAP-In marker cassette 1 with mScarlet-PuroTK

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Insert

    mScarlet-PuroTK
    

    Tags

    P2A

    Expression

    Bacterial and Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD-tdTEVDB_C-15A/G4C

    Plasmid

    #177205

    Purpose

    Permuted td intron with evolved initiation sequence for the expression of circular mRNA

    Depositor

    Stephen Fried

    Article

    Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175

    Insert

    Permuted GFP with evolved initiation sequence for circular mRNA
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Permuted sfGFP after residue 52 & Changed Cyt…

    Promoter

    araBAD

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pccGFPGpA

    Plasmid

    #73651

    Purpose

    Fluorescent reporter for transmembrane protein dimerization positive control.

    Depositor

    Alessandro Senes

    Article

    Armstrong et al Biochim Biophys Acta. 2016 Jul 21. pii:

    Insert

    glycophorin A
    

    Expression

    Bacterial

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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