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L4440_BioBrick-hif-1-sgRNA-A

Plasmid

#177784

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hif-1 (hif-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT001

Plasmid

#182711

Purpose

aTc inducible dCasRx

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

dCasRx

Use

CRISPR

Tags

2x-FLAG

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pTet

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pRK603

Plasmid

#8831

Depositor

David Waugh

Article

Kapust et al Protein Expr Purif. 2000 Jul . 19(2):312

Insert

TEV protease, S219D mutant

Expression

Bacterial

Mutation

S219D mutant

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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pAB094a

Plasmid

#79241

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PBAD rpoZ-HA4; PC araC

Expression

Bacterial

Promoter

PBAD; PC

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pH6HTC_ABCE1-Halo

Plasmid

#175335

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

ABCE1 (ABCE1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pHBintT

Plasmid

#48134

Purpose

Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vector

Depositor

Dieter Jahn

Article

Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCW-LIC-N8C_Fab3a

Plasmid

#203689

Purpose

Expresses N8C_Fab3a

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab3a

Expression

Bacterial

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pDSG419

Plasmid

#115600

Purpose

surface-displayed Ag2; medium copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSR83

Plasmid

#90211

Purpose

Expresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

Inserts

OmpR (ompR E. coli)
mCherry

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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His6-EGFP-PH-PLCD1

Plasmid

#183675

Purpose

PtdIns(4,5)P2 biosensor for purification from bacteria

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLCD1 (PLCD1 Human)

Tags

His6-EGFP

Expression

Bacterial

Mutation

Amino acids 11-140

Promoter

T7

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pOG231

Plasmid

#17736

Depositor

Geoff Wahl

Article

O'Gorman et al Proc Natl Acad Sci U S A. 1997 Dec 23;94

Insert

NLS-Cre recombinase

Use

Cre/Lox

Expression

Bacterial

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pWC2

Plasmid

#15960

Depositor

Jeff Gelles

Article

Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

Insert

Kinesin 1-401 BCCP his tag (Khc Fly, E. coli)

Tags

BCCP and His6

Expression

Bacterial

Mutation

amino acids 1-401

Available Since

Nov. 27, 2007

Availability

Academic Institutions and Nonprofits only

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TAF3 PHD_pGEX-6P-2

Plasmid

#92100

Purpose

Expresses GST fusion TAF3 PHD domain protein

Depositor

Albert Jeltsch

Article

Kungulovski et al Epigenetics Chromatin. 2016 Mar 22;9:11.

Insert

TAF3 PHD domain (TAF3 Human)

Tags

GST Tag

Expression

Bacterial

Promoter

tac promoter

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-SpRY-His

Plasmid

#179320

Purpose

Plasmid for bacterial expression and purification of SpRY-Cas9

Depositor

Julián Cerón, Miguel Angel Moreno-Mateos

Article

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Human codon-optimized Cas9 variant SpRY

Use

CRISPR

Tags

6xHis and SV40-NLS

Expression

Bacterial

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pTHSSe_60

Plasmid

#109254

Purpose

TALEsp2 stabilized promoter driving GFP

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

TALEsp2 stabilized promoter

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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PJQ47

Plasmid

#134646

Purpose

Contains Promoter 1 with bsa1 cut sites for inserting sgRNA

Depositor

Jason Peters, Oren Rosenberg

Article

Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Anderson BBa_J23117

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X27-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C

Plasmid

#159547

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12C

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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mPLD1-PX (71-210)

Plasmid

#119127

Purpose

Bacterial expression of human phox homology (PX) domain, mPLD1-PX (71-210)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

mPLD1-PX (71-210)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-Uricase

Plasmid

#182417

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-uricase fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-uricase

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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SL-SunTag

Plasmid

#196919

Purpose

The SunTag-Renilla-MS2 reporter containing cap-proximal GC-rich stem-loop

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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