32,069 results
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pORTMAGE312B
Plasmid#128969PurposeBroad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid inductionDepositorInsertsPapRecT
mutL E32K
ExpressionBacterialMutationE32KPromoterxylS-PmAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGSHU
Plasmid#72244PurposeContains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
hyg
I-SceI
ExpressionBacterialAvailable SinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBBR4pemIK
Plasmid#126928PurposeBroad host range vector with toxin-antitoxin stability system for maintenance in the absence of antibiotic selectionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
luxA+luxB/pRSETB Duet
Plasmid#197581PurposeExpressing luxA and luxB under dual T7 promoterDepositorInsertLuxA LuxB
TagsT7 tag and 6xHisExpressionBacterialPromoterT7Available SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKEW303-AcrVA5
Plasmid#115657PurposeExpresses AcrVA5 in bacteria, under TetR control.DepositorInsertAcrVA5
UseSynthetic BiologyExpressionBacterialPromoterPtetAvailable SinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Lac-dCas9 (Ptac)
Plasmid#105234PurposeTn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescensDepositorInsertS. pasteurianus dCas9
UseCRISPR and Synthetic BiologyTagsHAExpressionBacterialMutationD10A, H599AAvailable SinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC28-LIC-sfGFP-His
Plasmid#165479PurposepET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tagDepositorTypeEmpty backboneTagsTEV-GFP-6xHisExpressionBacterialAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLQ-Pxyl/tet-cas9-Pj23119-sgRNA
Plasmid#92121PurposeCRISPR-Cas9 based efficient genome editing in S. aureusDepositorInsertCas9-Pxyltet-sgRNA-pj23119
UseCRISPRExpressionBacterialAvailable SinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET151-HIS-TEV-GST-PP-RPN13
Plasmid#73741PurposeExpression of hRPN13 in E.coliDepositorAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRBBm34
Plasmid#48114PurposeExpression of the gfp gene under control of the native xylose inducible promoter of Bacillus megateriumDepositorInsertgfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mCherry (ErmR)
Plasmid#177206PurposePlasmid with p15A origin for constitutive mCherry expression in E. coliDepositorInsertmCherry
ExpressionBacterialPromoterSynthetic constitutive promoterAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX:PGRMC1
Plasmid#136062PurposeBacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.DepositorInsertProgesterone receptor membrane component 1 (PGRMC1 Human)
TagsGST-PGRMC1ExpressionBacterialAvailable SinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a.MJ0135
Plasmid#11412DepositorAvailable SinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_PCNA_IRES_puro2b
Plasmid#21048DepositorInserthPCNA
TagsGFPExpressionBacterial and MammalianAvailable SinceMay 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCS2TAL3-DD
Plasmid#37275PurposeTALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)DepositorTypeEmpty backboneTagsFlag, Fok I DD, Tal-C, and Tal-NExpressionBacterial and MammalianPromoterCMVAvailable SinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
His6_MBP_FXR1P
Plasmid#198694PurposeExpression of human FXR1 protein in E. coliDepositorAvailable SinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAK1
Plasmid#73244PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertAAK1 (AAK1 Human)
TagsTEV-His6-FlagExpressionBacterialMutationpfam00069:Pkinase 47-307PromoterT7Available SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nme2Cas9_pMCSG7
Plasmid#119925PurposeNme2Cas9 bacterial expression plasmidDepositorInsertNme2Cas9
TagsNLS, Nucleoplasmin NLS, and SV40 NLSExpressionBacterialPromoterT7Available SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSIBR004
Plasmid#177667PurposeSIBR Intron 4 (Int4) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterlacUV5Available SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only
