30,261 results

1. V72 ELP

   Plasmid

   #68938

   Purpose

   Elastin-like Polypeptide (VPGxG) with 72 repeats containing Valine guest residue

   Depositor

   Andrew MacKay

   Article

   Dhandhukia et al Biomacromolecules. 2013 Apr 8;14(4):976-

   Insert

   V72 ELP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-HIS-Xyl-Xmod-DocIII

   Plasmid

   #60865

   Purpose

   Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C

   Depositor

   Hermann Gaub

   Article

   Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

   Inserts

   Xyl
   Xmod-DocIII
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Threonine 120 to Cysteine in Xylanase domain

   Promoter

   T7

   Available since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ykgMO-IGS-sfGFP

   Plasmid

   #207231

   Purpose

   Calprotectin sensing promoter with sfGFP reporter

   Depositor

   Arthur Prindle

   Article

   Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

   Insert

   ykgMO-IGS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBP-lldR

   Plasmid

   #74095

   Purpose

   L-lactate repressor (LldR) from E. coli MG1655

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   lldR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

   Plasmid

   #169191

   Purpose

   To express nsp10-nsp16 fusion protein in E. coli

   Depositor

   John Diffley

   Article

   Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497

   Insert

   14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFP.R268

   Plasmid

   #138180

   Purpose

   Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(LNR).gp41-8.(SAV)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   P(araBAD) and iPC

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. 6CC8 (MBD3)

   Plasmid

   #119959

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   MBD3 (MBD3 Human)
   

   Use

   Tags

   GSTtag, 6xHis and Thrombin

   Expression

   Bacterial

   Mutation

   MBD domain (contains residues 1-71)

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pET51b_HCAII

   Plasmid

   #187110

   Purpose

   Expression of HCAII in bacteria

   Depositor

   Kai Johnsson

   Article

   Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

   Insert

   HCAII
   

   Use

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-PFO

   Plasmid

   #92109

   Purpose

   Recombinant expression in E.Coli

   Depositor

   Feng Shao

   Article

   Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

   Insert

   PFO
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Delta27aa

   Promoter

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-CV-SNAP25

    Plasmid

    #168031

    Purpose

    Expresses eCFP-SNAP25(A141-G206)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-SNAP25(A141-G206)-mVenus
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-Duet1_6xHis-TEV-eGFP-P62

    Plasmid

    #190929

    Purpose

    Plasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    P62 (SQSTM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJJS012

    Plasmid

    #188335

    Purpose

    PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    Linker::mTagBFP2::mIAA7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28b(+)_rPD-L1nb

    Plasmid

    #226715

    Purpose

    Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Recombinant PD-L1 nanobody
    

    Use

    Tags

    HA tag and Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTCF48 HLA-A*11:01

    Plasmid

    #180449

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*11:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mt deltaN20 TFB2MdeltaC3

    Plasmid

    #205062

    Purpose

    TFB2M protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    TFB2M (TFB2M Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks N-terminal 20 aa and C-terminal 3 aa

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSANG14-3F

    Plasmid

    #39265

    Depositor

    John McCafferty

    Article

    Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

    Type

    Empty backbone

    Use

    Tags

    6xHis, Alkaline phosphatase, pelB leader, and tri…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOPINS-UBE3C

    Plasmid

    #66711

    Purpose

    Expresses UBE3C HECT domain in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Insert

    KiAA0010 (UBE3C )
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pJJS001

    Plasmid

    #188324

    Purpose

    PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    mIAA7::GFP::Linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBAD-iLACCO1

    Plasmid

    #218817

    Purpose

    Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBAD-iLACCO1.1

    Plasmid

    #218818

    Purpose

    Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with low affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge