30,261 results

1. pC0067 PguCas13 (B11) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115218

   Purpose

   Expresses PguCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PguCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSumo-His6-SUMO-AtTPR1(1-209)

   Plasmid

   #177858

   Purpose

   Bacterial Expression of TPR1

   Depositor

   Karsten Melcher

   Article

   Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

   Insert

   TPR1 (TPR1 Mustard Weed)
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTHSSe_43

   Plasmid

   #109241

   Purpose

   pSC101 var1 BbsI cloning vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23105

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28-MHL: nsp10_SARS-CoV-2

   Plasmid

   #167851

   Purpose

   Bacterial expression of the SARS-CoV-2 protein nsp10

   Depositor

   Cheryl Arrowsmith

   Article

   Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

   Insert

   nsp10
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. PZac2.1 hSyn1-Lck-GFP

   Plasmid

   #177333

   Purpose

   Expresses GFP on the plasma membrane of neurons via an Lck localization sequence

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Lck-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   hSyn1

   Available since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX-XIAP

   Plasmid

   #8340

   Depositor

   Jon Ashwell

   Article

   Yang et al Science 2000 May 5;288(5467):874-7.

   Insert

   XIAP (XIAP Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBacPuroR-NeoR

   Plasmid

   #34921

   Depositor

   Ben Lehner

   Article

   Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

   Inserts

   Bacterial promoter::PuroR::bacterial 3p flank
   Bacterial promoter::NeoR::bacterial 3p flank
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQ-NFY-A-GB-HC

   Plasmid

   #171081

   Purpose

   Co-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYC

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Inserts

   NF-YA (NFYA Human)
   NF-YB (NFYB Human)
   NF-YC (NFYC Human)
   

   Use

   Tags

   GST and His

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDIV303

   Plasmid

   #204978

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and amdS selection marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   amdS
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   two silent mutations (nt177T>C, nt879C>T)

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBbB2k-GFP

    Plasmid

    #35345

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS mPax1 (CT#1007)

    Plasmid

    #13898

    Depositor

    Cliff Tabin

    Article

    Brent et al Cell. 2003 Apr 18. 113(2):235-48.

    Insert

    Pax1 (Pax1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETSXM2-pLacI-GFP

    Plasmid

    #174617

    Purpose

    Bacterial expression of GFP.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacI

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKM427

    Plasmid

    #210788

    Purpose

    L5 integrating vector for delivery of defective Hyg gene (ZeoR) for oligonucleotide-mediated SNP transfer

    Depositor

    Kenan Murphy

    Article

    Murphy et al Methods Mol Biol. 2021;2314:301-321. doi

    Insert

    Defective hygromycin resistant gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed hygromycin resistant gene W190 codon (TGG…

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAC1430

    Plasmid

    #127981

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCR8/GW/TOPO BMI

    Plasmid

    #69796

    Purpose

    Gateway entry vector for BMI

    Depositor

    Gerard Cagney

    Insert

    BMI (BMI1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET-6xHis-(pos9)GFP

    Plasmid

    #89247

    Purpose

    Expresses GFP with +9 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos9)-GFP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mT-Sapphire-pBAD

    Plasmid

    #54571

    Purpose

    Localization: Protein Expression Vector , Excitation: 399, Emission: 511

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

    Type

    Empty backbone

    Use

    Tags

    6xHis and mT-Sapphire

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pBT100.106b

    Plasmid

    #122601

    Purpose

    clone APOBEC1 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    APOBEC1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-TRE3G-SOX17

    Plasmid

    #104541

    Purpose

    PiggyBac vector encoding doxycycline-inducible human SOX17

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    SRY-box 17 (SOX17 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET His6 Gamma crystallin TEV LIC cloning vector (1X)

    Plasmid

    #29661

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, Gamma-crystallin, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge