30,807 results

1. pET21-pelB-LaRIgG-2

   Plasmid

   #228996

   Purpose

   For bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaRIgG-2
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaGIgG-12

   Plasmid

   #228999

   Purpose

   For bacterial expression of anti-mouse IgG nanobody LaMIgG-12, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaGIgG-12
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-pelB-GST-3

   Plasmid

   #228985

   Purpose

   For bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-3
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. MZB134

   Plasmid

   #228485

   Purpose

   "Empty" N-terminal 6xHis-TEV-Halotag-HRV vector for easy DNA Assembly for pulldowns (Bacterial Expression)

   Depositor

   Richard Baker

   Type

   Empty backbone

   Tags

   6xHis-TEV-Halo-HRV

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTBY1-T4uvsY

   Plasmid

   #228214

   Purpose

   Expression in BL21(DE3) of T4 UvsY-like recombination mediator

   Depositor

   Stephen Benkovic

   Insert

   uvsY (uvsY Escherichia phage T4)
   

   Tags

   Sce VMA intein-chitin binding domain

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-SnoopTag-AffiHER2-SpyTag

   Plasmid

   #216280

   Purpose

   Expresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SnoopTag-AffiHER2-SpyTag
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6, SnoopTag, SpyTag, and Thrombin cleavage sit…

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDB.His.MBP.3C CARD8 CARD WT

   Plasmid

   #164022

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA-CARD

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMUT2-pCadBA-mCherry

   Plasmid

   #192865

   Purpose

   pMUT2 based vector for sensing pH in E. coli Nissle 1917

   Depositor

   Morten Sommer

   Article

   Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

   Insert

   pH sensitive promoter
   

   Expression

   Bacterial

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22b-csgB

   Plasmid

   #89582

   Purpose

   Express csgB curli protein with GS linker and Histaq

   Depositor

   Urartu Seker

   Article

   Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

   Insert

   csgB (csgB )
   

   Tags

   3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

   Expression

   Bacterial

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET32a-Trx-His6-TEV_SacB

    Plasmid

    #164588

    Purpose

    Bacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.

    Depositor

    Ernst Schonbrunn

    Insert

    TEV-SacB
    

    Tags

    Trx-His6 followed by TEV site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-6xHis-(pos14)GFP

    Plasmid

    #89252

    Purpose

    Expresses GFP with +14 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos16)-GFP
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PZac2.1 hSyn1-Lck-13xLinker-BioID2-BioID2-HA

    Plasmid

    #176854

    Purpose

    Expresses membrane bound biotin ligase, BioID2, in neurons

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Lck-13xLinker-BioID2-BioID2-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Promoter

    hSyn1

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSP712

    Plasmid

    #65768

    Purpose

    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. HLA-A2.1

    Plasmid

    #14904

    Depositor

    Linda Sherman

    Article

    Irwin et al J Exp Med. 1989 Oct 1. 170(4):1091-101.

    Insert

    HLA-A2.1 (HLA-A Human)
    

    Expression

    Bacterial

    Available Since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRBAD-Z-CGFP

    Plasmid

    #52735

    Purpose

    L-(+)-arabinose-inducible expression of Z-CGFP positive control for in vivo split GFP assembly assay

    Depositor

    Lynne Regan

    Article

    Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

    Insert

    Z+CGFP
    

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLS.405

    Plasmid

    #187203

    Purpose

    Expresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA (wildtype)
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7/Lac

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXs-Runx1

    Plasmid

    #131609

    Purpose

    expression of mouse Runx1 in mammalian cells

    Depositor

    Kateri Moore, Filipe Pereira

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Runx1 (Runx1 Mouse)
    

    Use

    Retroviral

    Expression

    Bacterial

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. TS246_pNKI-2-13_HALO-DPN7

    Plasmid

    #200720

    Purpose

    producing Methyl Tracer HALO protein in bacteria

    Depositor

    Bas van Steensel

    Article

    van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

    Insert

    HALO-DPN7
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    codon optimized

    Available Since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28_6xHis-PARP1-E988K

    Plasmid

    #173945

    Purpose

    Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-E988K (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    V762A, E988K

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZac2.1 gfaABC1D-Ezr-eGFP

    Plasmid

    #176856

    Purpose

    Expresses Ezrin fused with eGFP at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Ezr-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Bacterial

    Promoter

    gfaABC1D

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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