30,807 results

1. pET21b-Caspase-3(C163S)

   Plasmid

   #90088

   Purpose

   Expresses caspase 3 with C163S to prevent autocatalysis in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

   Insert

   Caspase 3 (CASP3 Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   C163S

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. JMJD3

   Plasmid

   #39069

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   JMJD3 (KDM6B Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pAWP78-PVCpnf_pvc13-truncated

   Plasmid

   #198286

   Purpose

   Contains a tail fiber with a truncated binding domain (lacking aa403-476 in Pvc13); produces nontargeting PVCs

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-truncated
   

   Expression

   Bacterial

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJCX3-ACTG2_R257C

   Plasmid

   #228911

   Purpose

   Expresses mutant R257C smooth muscle actin gamma-2 in human cells

   Depositor

   Roberto Dominguez

   Insert

   Actin gamma 2, smooth muscle (ACTG2 Human)
   

   Tags

   HIS-FLAG-TEV

   Expression

   Bacterial and Mammalian

   Mutation

   R257C

   Promoter

   CMV

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJCX3-ACTG2_WT

   Plasmid

   #228910

   Purpose

   Expresses wildtype smooth muscle actin gamma-2 in human cells

   Depositor

   Roberto Dominguez

   Insert

   Actin gamma 2, smooth muscle (ACTG2 Human)
   

   Tags

   HIS-FLAG-TEV

   Expression

   Bacterial and Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTriEx-mCherry::LANS4

   Plasmid

   #60785

   Purpose

   Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

   Insert

   LANS4
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_CTTN-1/1

   Plasmid

   #91550

   Purpose

   Protein expression and purification of human SH3 domain construct CTTN-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   CTTN-1/1 (CTTN Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. SB_convex

   Plasmid

   #110104

   Purpose

   pBXPHM3 with scaffold Sybody of the convex library

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Insert

   scaffold Sybody of the convex library
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   May 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPGC-K

   Plasmid

   #174581

   Purpose

   pOPIN-GG expression vector compatible with C-terminal tagging, Kanamycin resistant

   Depositor

   Mark Banfield

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pH6HTC_IRP1-Halo

    Plasmid

    #175305

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    IRP1 (ACO1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-29b(+)-mSandy2

    Plasmid

    #177760

    Purpose

    Expresses mSandy2 using the T7 Promoter

    Depositor

    Roberto Chica

    Article

    Legault et al Chem Sci. 2022 Jan 11;13(5):1408-1418. d

    Insert

    mSandy2
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETDuet-AtRBX1-T7-AtSKP1-T7-AtCUL1-S

    Plasmid

    #238003

    Purpose

    Expression AtRBX1-T7, AtSKP1-T7 and AtCUL1-S in in bacterial cells

    Depositor

    Lei Wu

    Article

    Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

    Inserts

    AtRBX1 (RBX1 Mustard Weed)
    AtSKP1 (SKP1 Mustard Weed)
    AtCUL1 (CUL1 Mustard Weed)
    

    Tags

    S and T7

    Expression

    Bacterial

    Available Since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYO1125

    Plasmid

    #235742

    Purpose

    Protein expression of GST-tagged PX domain (Cys at 2) in bacteria

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    p40 PX domain (NCF4 Human)
    

    Tags

    GST-TEV

    Expression

    Bacterial

    Mutation

    aa 2-149 with Cys at 2

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSF-MjCouRS

    Plasmid

    #229066

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    Methanococcus jannaschii tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGal-Trans-Myc-eGFP-KDEL

    Plasmid

    #177729

    Purpose

    ER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)
    

    Tags

    KDEL, Myc, and eGFP

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. R701-X72-527: His6-tev-Hs.CALM1(76-148)

    Plasmid

    #159695

    Purpose

    E. coli protein expression of His6-tev-Hs.CALM1(76-148)

    Depositor

    Dominic Esposito

    Article

    Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

    Insert

    His6-tev-Hs.CALM1(76-148) (CALM1 Human)
    

    Tags

    His6-tev

    Expression

    Bacterial

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHHM.X513-iNicSnFR3b-(V7)

    Plasmid

    #124881

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

    Depositor

    Henry Lester

    Article

    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

    Insert

    iNicSnFR3b-(V7)
    

    Tags

    HA Tag, His Tag, and NA

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    T7

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pspCas9-Klenow-ST2-com-vector

    Plasmid

    #176235

    Purpose

    Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)

    Plasmid

    #52054

    Purpose

    Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)

    Depositor

    Jesse Rinehart

    Inserts

    SepRS
    EF-Sep
    5x tRNA Sep
    

    Expression

    Bacterial

    Mutation

    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

    Promoter

    Ptac

    Available Since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-2(KW)2-mCherry

    Plasmid

    #112738

    Purpose

    Express 2(KW)2-mCherry in E. coli

    Depositor

    Kyubong Jo

    Article

    Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

    Insert

    2(KW)2-mCherry
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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