30,261 results

1. TOPO GFP

   Plasmid

   #68488

   Purpose

   TOPO construct expressing GFP for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Green Fluorescent Protein
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEA01

   Plasmid

   #170766

   Purpose

   Utilisation of pSAM2 excision tool in Amycolatopsis spp

   Depositor

   Jean-Luc Pernodet

   Article

   Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

   Inserts

   xis, int
   oriT
   pac
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trcp

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQ-NFY-A-GB-HC

   Plasmid

   #171081

   Purpose

   Co-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYC

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Inserts

   NF-YA (NFYA Human)
   NF-YB (NFYB Human)
   NF-YC (NFYC Human)
   

   Use

   Tags

   GST and His

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PZac2.1 hSyn1-Lck-GFP

   Plasmid

   #177333

   Purpose

   Expresses GFP on the plasma membrane of neurons via an Lck localization sequence

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Lck-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   hSyn1

   Available since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBbB2k-GFP

   Plasmid

   #35345

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC0067 PguCas13 (B11) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115218

   Purpose

   Expresses PguCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PguCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-6xHis-(pos9)GFP

   Plasmid

   #89247

   Purpose

   Expresses GFP with +9 theoretical charge in E.Coli

   Depositor

   David Liu

   Article

   Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

   Insert

   His6-(pos9)-GFP
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Paxillin

   Plasmid

   #186142

   Purpose

   For expresseion of recombinant paxillin in bacteria

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   PXN (PXN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Paxillin C1997A (silent mutation for cloning)

   Promoter

   Lac

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDIV303

   Plasmid

   #204978

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and amdS selection marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   amdS
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   two silent mutations (nt177T>C, nt879C>T)

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTHSSe_43

    Plasmid

    #109241

    Purpose

    pSC101 var1 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23105

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSumo-His6-SUMO-AtTPR1(1-209)

    Plasmid

    #177858

    Purpose

    Bacterial Expression of TPR1

    Depositor

    Karsten Melcher

    Article

    Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

    Insert

    TPR1 (TPR1 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-TRE3G-SOX17

    Plasmid

    #104541

    Purpose

    PiggyBac vector encoding doxycycline-inducible human SOX17

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    SRY-box 17 (SOX17 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-MHL: nsp10_SARS-CoV-2

    Plasmid

    #167851

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp10

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pAC1430

    Plasmid

    #127981

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. mT-Sapphire-pBAD

    Plasmid

    #54571

    Purpose

    Localization: Protein Expression Vector , Excitation: 399, Emission: 511

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

    Type

    Empty backbone

    Use

    Tags

    6xHis and mT-Sapphire

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pETSXM2-pLacI-GFP

    Plasmid

    #174617

    Purpose

    Bacterial expression of GFP.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacI

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBacPuroR-NeoR

    Plasmid

    #34921

    Depositor

    Ben Lehner

    Article

    Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

    Inserts

    Bacterial promoter::PuroR::bacterial 3p flank
    Bacterial promoter::NeoR::bacterial 3p flank
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-T4uvsW

    Plasmid

    #228201

    Purpose

    Expression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealase

    Depositor

    Stephen Benkovic

    Article

    Nelson et al J Biol Chem. 2007 Jan 5;282(1):407-16. d

    Insert

    uvsW
    

    Use

    Tags

    hexahistidine and 10 amino acid linker

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBT100.106b

    Plasmid

    #122601

    Purpose

    clone APOBEC1 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    APOBEC1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

    Plasmid

    #130640

    Purpose

    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on TniQ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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