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pET30-2-PSAT1

Plasmid

#83908

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

phosphoserine aminotransferase 1 (PSAT1 Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pIB185

Plasmid

#90196

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

Depositor

Indranil Biswas

Article

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

None

Expression

Bacterial

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD33.lon

Plasmid

#22145

Depositor

Robert Sauer

Article

Gur et al Genes Dev. 2008 Aug 15. 22(16):2267-77.

Insert

Lon (lon E. coli K12)

Expression

Bacterial

Mutation

None, WT gene

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pKEW336-MBP-TEV-MbCas12a-33362

Plasmid

#115670

Purpose

Expresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

MbCas12a

Use

CRISPR

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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CH-GECO2.1

Plasmid

#52099

Purpose

mCherry-based Calcium indicators

Depositor

Robert Campbell

Article

Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

Insert

CH-GECO2.1

Tags

6 His tag

Expression

Bacterial

Promoter

pBAD promotor

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pBad33_ISDge10_reRNA

Plasmid

#205340

Purpose

Bacterial expression of ISDge10 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISDge10 TnpB

Expression

Bacterial

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMDH

Plasmid

#133129

Purpose

A recombinant plasmid expressing mdh (malate dehydrogenase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

malate dehydrogenase gene

Expression

Bacterial

Promoter

malate dehydrogenase promoter

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD_GFP11_T7LysH17A

Plasmid

#59591

Purpose

Expression vector for solubility screening of proteins or protein fragments using a split-GFP assay.

Depositor

Richard Kingston

Article

Bulloch et al Anal Biochem. 2014 Jul 10. pii: S0003-26

Type

Empty backbone

Tags

6xHis-tag and GFP11

Expression

Bacterial

Promoter

pARA

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin WT

Plasmid

#45969

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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AcpP pet29a C-terminal His Tag

Plasmid

#75016

Purpose

Expresses the acyl carrier protein (ACP) from the E. coli fatty acid synthase

Depositor

Michael Burkart

Article

Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

Insert

AcpP (acpP E. coli)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
pTCF5 HLA-A*01:01

Plasmid

#180448

Purpose

E. coli expression of soluble MHC protein

Depositor

John Altman

Insert

HLA-A*01:01

Tags

BSP41

Expression

Bacterial

Mutation

Replacement of transmembrane domain with enzymati…

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp3 C163A-His

Plasmid

#11822

Depositor

Guy Salvesen

Article

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 3 catalytic mutant (CASP3 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C163A

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
STARD5A

Plasmid

#42392

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

STARD5A (STARD5 Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
Keasling-2337

Plasmid

#100168

Purpose

Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

Depositor

Jay Keasling

Article

Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

Insert

lacIq-Ptrc-ADS

Expression

Bacterial

Promoter

trc

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pJC20Tpm1

Plasmid

#99355

Purpose

Bacterial expression of budding yeast (S. cerevisiae) Tropomyosin, Tpm1

Depositor

Dan Mulvihill

Article

Skoumpla et al J Cell Sci. 2007 May 1;120(Pt 9):1635-45

Insert

Tropomyosin (TPM1 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1

Plasmid

#187830

Purpose

Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST and TEV-mEGFP-GG Linker

Expression

Bacterial and Insect

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-SnoopTagJr-MBP

Plasmid

#105628

Purpose

Plasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigase

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

SnoopTagJr-MBP

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBbS5k-RFP

Plasmid

#35285

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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K560GFP

Plasmid

#129761

Purpose

Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays

Depositor

William Hancock

Insert

KHC (Khc Fly)

Tags

GFP and His6

Expression

Bacterial

Mutation

Truncation at 559

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCN34e-kimA-yfp

Plasmid

#227638

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

kimA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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