32,826 results
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Plasmid#197985PurposeExpresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag.DepositorInsertMBP-t-M.MpeI-N374K-His
TagsHis and MBPExpressionBacterialMutationM.MpeI-N374KAvailable SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR1_DVK_AE(J23100-BCD2-E0040m-B0015)
Plasmid#197660PurposeExpresses GFP under the control of J23100 and BCD2DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hTGFa
Plasmid#199236PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertTransforming growth factor alpha, partial (TGFA Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTarget: Ptaq-RFP_PlacIq-GFP
Plasmid#192280PurposeRFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter PlaciqDepositorInsertsRFP
GFP
ExpressionBacterialPromoterPlacIq and PtaqAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPARA2
Plasmid#200417PurposeGolden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)DepositorArticleTypeEmpty backboneExpressionBacterialPromoterpBADAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
CJ8421_04975 Rptr2 recording plasmid
Plasmid#194211PurposeCJ8421_04975 Rptr2 targeted DNA sequenceDepositorInsertCJ8421_04975 Rptr2 DNA target
ExpressionBacterialAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CRY2Clust-FUSN-mut
Plasmid#200970Purposeinduction of phase-separated condensate mediated by FUSN-IDR mutantDepositorInsertFUSNmut
UseLentiviralExpressionBacterial and MammalianMutation15 Tyr-to-Ser substitutionsAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CRY2Clust
Plasmid#200969Purposeinduction of phase-separated condensate by CRY2ClustDepositorInsertCRY2Clust
UseLentiviralExpressionBacterial and MammalianAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-mKate2
Plasmid#199249PurposeFluorescent labelling of bacterial cells with mKate2DepositorInsertCodon-optimized mKate2 gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMA-Thermal_CTLA-4
Plasmid#199751PurposeThermal expression of CTLA-4DepositorInsertCTLA-4 (Ctla4 Mouse)
ExpressionBacterialAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHEN2 F8-2
Plasmid#186033PurposeExpress VHH F8-2 with 3 c-myc tag in E. coli periplasmDepositorInsertVHH F8-2
Tags6His and MycExpressionBacterialPromoterlacAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2
Plasmid#196250PurposePlasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119DepositorInsertattL1-GlpT-scGFP-attL2
ExpressionBacterialPromoterJ23119 promoterAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
His6_MBP_FXR2P_P474S_P493N
Plasmid#198695PurposeExpression of human FXR2 protein in E. coliDepositorInsertFXR2P (FXR2 Human)
TagsMBPExpressionBacterialMutationChanged proline 474 to serine and proline 493 asp…PromoterT7Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10Smt3-gamma-toxin
Plasmid#197000PurposeBacterial expression plasmid for gamma toxin tRNA endonucleaseDepositorInsertGamma toxin
TagsHis10-Smt3ExpressionBacterialAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDSP33
Plasmid#199375PurposeEncodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
ExpressionBacterialMutationno mutations, except added subcellular localizati…Available SinceApril 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHJL2
Plasmid#199377PurposeEncodes dual cistronic cytosolic GCaMP6f and mScarlet-I optimized for C. elegans expressionDepositorInsertGCaMP6f::SL2::mScarlet-I
ExpressionBacterialMutationno mutationsAvailable SinceApril 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET24b-BR1.0
Plasmid#199435Purposeexpresses BAmRed1.0 in bacterial cellsDepositorInsertBAMRed
Tagshis tagExpressionBacterialPromoterT7Available SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
TwinStrep-SUMO-Pdp1
Plasmid#198320PurposeFor affinity purification of Pdp1 payloadDepositorInsertTwinStrep-SUMO-Pdp1
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnfΔpvc15_pvc12-HiBiT
Plasmid#198309PurposeNegative control for payload loading assay; produces PVCs deficient for payload loader (pvc15)DepositorInsertPVCpnfΔpvc15_pvc12-HiBiT
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
ALIX-His(1-868, P801G)
Plasmid#186808PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).DepositorInsertALIX-His (1-868, P801G) (PDCD6IP Human)
TagsHis6ExpressionBacterialMutationProline 801 to GlycineAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-6P-1-HsRCK_E
Plasmid#146210PurposeBacterial Expression of HsRckDepositorInsertHsRck (DDX6 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miRFP720-HO1
Plasmid#197257PurposeExpresses the protein of miRFP720 and HO1 in bacteriaDepositorInsertmiRFP720
ExpressionBacterialAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miRFP680-HO1
Plasmid#197253PurposeExpresses the protein of miRFP680 and HO1 in bacteriaDepositorInsertmiRFP680
ExpressionBacterialAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29_LuxSit_i
Plasmid#194299PurposeBacterial expression of the de novo luciferase, LuxSit-iDepositorInsertLuxSit-i
ExpressionBacterialAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
p6187
Plasmid#198158Purposebacterial expression of sifA::HaloTag fusion, mutant formDepositorInsertPsifA sifA delta331-336::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)
TagsHaloTag-HAExpressionBacterialAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Po-nCas9-BE
Plasmid#195740PurposeExpress APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coliDepositorArticleInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPo (phoenol-inducible promoter)Available SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-KRaION1
Plasmid#177799PurposeBacterial expression of green fluorescent potassium indicator KRaION1DepositorInsertKRaION1
ExpressionBacterialPromoteraraBAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pET22b-T5-sfGFP*- MmH-MH4R
Plasmid#196409PurposeExpress sfGFP with TAG and enzymes for biosynthesizing DOPADepositorInsertsExpressionBacterialAvailable SinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only