32,167 results

Showing: 8461 - 8490 of 32167 results

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pET30b-7Y-SrtA

Plasmid

#105601

Purpose

expresses 7Y sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7Y mutant

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pPBC-LG3-tdT

Plasmid

#62810

Purpose

pPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.

Depositor

Karen Wilcox

Article

Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

Insert

ITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET22-His-TEV-DOT1L

Plasmid

#89610

Purpose

Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416

Depositor

Leemor Joshua-Tor

Article

Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

Insert

Dot1L (DOT1L Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAD6

Plasmid

#134349

Purpose

E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

Depositor

Seth Darst

Article

Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

Insert

mfd (mfd E. coli)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBBR5pemIKpBAD

Plasmid

#129273

Purpose

Broad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and arabinose-inducible promoter

Depositor

Lindsey Burbank

Article

Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-30

Plasmid

#172754

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-30

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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PNMT

Plasmid

#64078

Purpose

Expression of human Phenylethanolamine N-methyltransferase

Depositor

Vern Schramm

Article

Stratton et al ACS Chem Biol. 2017 Feb 17;12(2):342-346

Insert

Phenylethanolamine N-methyltransferase (PNMT Synthetic, Human)

Tags

Histidine tag

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only
pTEV5_ RpMatB_1

Plasmid

#78205

Purpose

Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor

Depositor

Martin Webb

Article

Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7

Insert

matB (His6 C106A R286C Q457C K488A)

Tags

His6

Expression

Bacterial

Mutation

C106A R286C Q457C K488A

Promoter

T7

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only
pLITMUS*-PrpoN-CroAct3 63aa-PBBa_J23106-geneIII

Plasmid

#134355

Purpose

Phagemid

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

CroAct3 63aa

Expression

Bacterial

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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PclpB riboswitch GFP pZa

Plasmid

#170078

Purpose

GFP expression under the control of E. coli clpB promoter engineered with riboregulators

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PclpB

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETB PKA Cat

Plasmid

#14920

Depositor

Susan Taylor

Article

Taylor et al Biochim Biophys Acta. 2005 Dec 30. 1754(

Insert

PKA catalytic subunit alpha (Prkaca Mouse)

Expression

Bacterial

Available Since

July 24, 2007

Availability

Academic Institutions and Nonprofits only
pHH0103_SPF45

Plasmid

#110044

Purpose

Protein expression and purification of SPF45

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SPF45 (RBM17 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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TRBV18

Plasmid

#123452

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV18 (TRBV18 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV15

Plasmid

#123450

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV15 (TRBV15 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV10-3

Plasmid

#123441

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV10-3 (TRBV10-3 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV7-2

Plasmid

#123431

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV7-2 (TRBV7-2 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV6-1

Plasmid

#123421

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV6-1 (TRBV6-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV4-2

Plasmid

#123412

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV4-2 (TRBV4-2 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TRBV4-1

Plasmid

#123411

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV4-1 (TRBV4-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TRAV38-2

Plasmid

#123405

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV38-2 (TRAV38-2DV8 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TRAV22

Plasmid

#123394

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV22 (TRAV22 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TRAV20

Plasmid

#123392

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV20 (TRAV20 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV3-1

Plasmid

#123410

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV3-1 (TRBV3-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TRAV8-2

Plasmid

#123376

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV8-2 (TRAV8-2 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TRAV4

Plasmid

#123371

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV4 (TRAV4 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TRAV1-2

Plasmid

#123368

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV1-2 (TRAV1-2 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pJK226

Plasmid

#71125

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pQTEV-SNX3

Plasmid

#31573

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

sorting nexin 3 (SNX3 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pETNKI-strepII-3C-LIC-Amp

Plasmid

#108707

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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VPS4B, S2C

Plasmid

#184806

Purpose

Bacterial expression of VPS4B (S2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

VPS4B (VPS4B Human)

Tags

His6

Expression

Bacterial

Mutation

S2C

Promoter

T7

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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