30,807 results

1. pGEX-4T1_4xUb

   Plasmid

   #199779

   Purpose

   Used for the expression of 4X human ubiquitin in tandem (SMC interal no. 538)

   Depositor

   Sascha Martens

   Article

   Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

   Insert

   4X Ubiquitin in Tandem
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBMCS-SortA

   Plasmid

   #21931

   Depositor

   Fuyuhiko Inagaki

   Article

   Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

   Insert

   sortase (srtA Staphylococcus aureus (strain N315))
   

   Tags

   GB1 and His-tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59

   Available Since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

3. OTUD5

   Plasmid

   #28270

   Depositor

   Cheryl Arrowsmith

   Insert

   OTUD5 (OTUD5 Human)
   

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   OTU DOMAIN (residues 172-339).

   Available Since

   Feb. 10, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

4. SB_loop

   Plasmid

   #110103

   Purpose

   pBXPHM3 with scaffold Sybody of the loop library

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Insert

   scaffold Sybody of the loop library
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   May 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pProEx Rab5C(S30C) CL

   Plasmid

   #224273

   Purpose

   Protein purification of Rab5C (S30C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Rab5C (RAB5C Human)
   

   Expression

   Bacterial

   Mutation

   S30C

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-sfGFP-DogTag Loop A

   Plasmid

   #171779

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-DogTag Loop A
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   DogTag and linkers inserted between residues Val2…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28b-miniE-DBCO

   Plasmid

   #181985

   Purpose

   Used to label proteins with the MiniR genetically encoded tag

   Depositor

   Kimberly Beatty

   Article

   Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

   Insert

   MiniE-DBCO
   

   Tags

   DBCO-Tag, His-Tag

   Expression

   Bacterial

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR8-attR4-pegRNA-attR3

   Plasmid

   #213056

   Purpose

   Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Insert

   sgRNA
   

   Expression

   Bacterial

   Promoter

   35S-CmYCLV-AtU6

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR8-attL5-pegRNA-attL4

   Plasmid

   #213055

   Purpose

   Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Insert

   sgRNA
   

   Expression

   Bacterial

   Promoter

   35S-CmYCLV-AtU6

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR8-attL1-pegRNA-attR5

    Plasmid

    #213053

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGal CycK (P#844)

    Plasmid

    #14664

    Depositor

    Matija Peterlin

    Article

    Lin et al J Biol Chem. 2002 May 10. 277(19):16873-

    Insert

    CycK (CCNK Human)
    

    Expression

    Bacterial

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pKL09-GCaMP6f-mScarlet bb118

    Plasmid

    #158979

    Purpose

    Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    GCaMP6f-mScarlet fusion
    

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#100833

    Promoter

    Bba_J23118 (sigma 70)

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. MUNC18a

    Plasmid

    #218888

    Purpose

    Expresses rat MUNC18a in bacteria

    Depositor

    Axel Brunger

    Article

    Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

    Insert

    Munc18a (Stxbp1 Rat)
    

    Tags

    6 his

    Expression

    Bacterial

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP-mCherry-Ki67

    Plasmid

    #183750

    Purpose

    Expresses EGFP-mCherry-Ki67

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 (MKI67 Human)
    

    Tags

    EGFP and mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWUKI 1+2

    Plasmid

    #79879

    Purpose

    Encodes Cas1+2 and a CRISPR array

    Depositor

    George Church

    Article

    Shipman et al Science. 2016 Jun 9. pii: aaf1175.

    Inserts

    Cas1
    Cas2
    CRISPR Array
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

    Plasmid

    #130640

    Purpose

    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on TniQ

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28c-Ku

    Plasmid

    #161936

    Purpose

    Expresses 6xHis-tagged Ku from Mycobacterium tuberculosis under T7 promoter for purification

    Depositor

    Harris Wang

    Article

    Yim et al ACS Synth Biol. 2020 Sep 29. doi: 10.102

    Insert

    ku (mku Mycobacterium tuberculosis)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLG018 (AVAST type 2)

    Plasmid

    #157896

    Purpose

    Expresses the AVAST type 2 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    AVAST type 2 (STAND ATPase with uncharacterized helical N-terminal domain)
    

    Expression

    Bacterial

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-6xHis-(pos9)GFP

    Plasmid

    #89247

    Purpose

    Expresses GFP with +9 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos9)-GFP
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEM112B

    Plasmid

    #210602

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Ater-mClover3-SpH2Apro | SpH2Bpro-GP2A-GT2A-Hshph-Synter8
    

    Tags

    mClover3

    Expression

    Bacterial and Yeast

    Promoter

    SpH2A/Bpro (divergent)

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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