30,263 results

1. pSA1

   Plasmid

   #52261

   Purpose

   Bacterial expression of human 6His-tagged SUMO1 and SUMO-E1

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Inserts

   SUMO-1 (SUMO1 Human)
   SAE-2 (UBA2 Human)
   SAE-1 (SAE1 Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   C-terminal gly-gly

   Promoter

   T7 and rbs

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1a. pETM11-SUMO-SNCA-GFP

   Plasmid

   #107292

   Purpose

   Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

   Depositor

   Dmytro Yushchenko

   Article

   Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

   Insert

   SNCA-GFP (SNCA Human)
   

   Use

   Tags

   His-tag and SUMO-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIC-CH

   Plasmid

   #26117

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Type

   Empty backbone

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO

   Available since

   Sept. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. EV-D68 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

   Plasmid

   #204817

   Purpose

   Bacterial expresison of Enterovirus D68 3C protease

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   EV D68 3C protease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. TARG1-c002

   Plasmid

   #173108

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   TARG1
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   Full length protein

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKD233.7-3

   Plasmid

   #90955

   Purpose

   Expresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutively

   Depositor

   Jeffrey Tabor

   Article

   Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

   Inserts

   Tetrathionate response regulator
   Superfolder GFP
   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bba_J23114, PltetO-1, and PttrB185-269

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-Rac1

   Plasmid

   #12200

   Depositor

   Jonathan Chernoff

   Article

   Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Starting methionine (M1) removed; F78S compared t…

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH6HTC_PTBP1-Halo

   Plasmid

   #175302

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   PTBP1 (PTBP1 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCHARGE3

   Plasmid

   #24658

   Depositor

   Tanya Parish

   Article

   Carroll et al PLoS One. 2010 . 5(3):e9823.

   Insert

   Turbo
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKAR2-Br512g3.2

    Plasmid

    #171201

    Purpose

    Expresses Br512g3.2 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Bhadra et al medRxiv 2022.08.09.22278605

    Insert

    Engineered Bst DNA Polymerase I Large Fragment
    

    Use

    Tags

    8x His tag; Villin Headpiece 47 amino acid (vHP47…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPbcas13b

    Plasmid

    #89906

    Purpose

    Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    Cas13b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2T-CAG-SpCas9-BlastR

    Plasmid

    #107190

    Purpose

    Confers constitutive expression of SpCas9

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Insert

    SpCas9 (NEWENTRY )
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Chicken ß-actin

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAD12

    Plasmid

    #34832

    Depositor

    Cynthia Kenyon

    Article

    Dillin et al Science. 2002 Oct 25;298(5594):830-4.

    Insert

    None
    

    Use

    RNAi

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2012

    Availability

    Academic Institutions and Nonprofits only

14. p1A

    Plasmid

    #162692

    Purpose

    Expresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM-30-GST-PfAgo_F

    Plasmid

    #146265

    Purpose

    Bacterial Expression of PfAgo

    Depositor

    Elisa Izaurralde

    Insert

    PfAgo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one silent T1704C and one non silent mutation A90…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDonor14-ShoHELIX-KanR (BO4)

    Plasmid

    #181795

    Purpose

    pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAM552

    Plasmid

    #154131

    Purpose

    Expresses wt rRNAs operon in bacterial cells

    Depositor

    Alexander Mankin

    Article

    Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

    Insert

    wt bacterial rRNAs operon under control of pL promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSRK2721

    Plasmid

    #78572

    Purpose

    Gateway destination vector for production of MBP-3C site-His6-TEV site-passenger protein fusions

    Depositor

    David Waugh

    Article

    Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

    Insert

    Gateway cloning cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRF-TetR

    Plasmid

    #49374

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    TetR repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACYC-T7-Nla-repeat-BbsI

    Plasmid

    #180215

    Purpose

    For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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