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32,163 results

Showing: 11401 - 11440 of 32163 results

  1. pZH517

    Plasmid
    #102670
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102670-plasmid-map-sequence-id-199582

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  3. pET15-EF1p_mFAP2b

    Plasmid
    #159469
    Purpose
    Encodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF1p_mFAP2b
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Available Since
    Dec. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    159469-plasmid-map-sequence-id-319725

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  13. SpIMPDH

    Plasmid
    #206980
    Purpose
    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.
    Depositor
    Vern Schramm
    Article
    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210
    Insert
    SpIMPDH (guaB Streptococcus pyogenes)
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Available Since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    206980-plasmid-map-sequence-id-410397

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  14. T7RNAP Cyslight A188C

    Plasmid
    #205083
    Purpose
    T7RNAP protein expression
    Depositor
    Smita Patel
    Insert
    T7RNAP Cyslight A188C (T7p07 Bacteriophage T7)
    Expression
    Bacterial
    Mutation
    C125S, C347S, C510S, C515S, C530S, C723S N88C
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205083-plasmid-map-sequence-id-413708

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  19. pDule-IBBN (G2)

    Plasmid
    #85500
    Purpose
    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-
    Insert
    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32G L65E F108W Q109M D158S R162K
    Promoter
    lpp (constitutive)
    Available Since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85500-plasmid-map-sequence-id-169863

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  20. pBV58

    Plasmid
    #204603
    Purpose
    ERG11-3X Minidegron-5X FLAG-HIS3MX6
    Depositor
    Scott Moye-Rowley
    Insert
    Candida glabrata ERG11 (ERG11 )
    Tags
    auxin-inducible degron
    Expression
    Bacterial
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    204603-plasmid-map-sequence-id-403443

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  24. p405CYC1

    Plasmid
    #15967
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    p405CYC1 (CYC1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    CYC1 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15967-plasmid-map-sequence-id-8452

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  26. pHN873

    Plasmid
    #178829
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Tags
    MBP, His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178829-plasmid-map-sequence-id-351932

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  28. DRP2A

    Plasmid
    #42354
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DRP2A (DPYSL2 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  35. pTJV1Sc2-tetA

    Plasmid
    #166982
    Purpose
    Generates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    CspRecT
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    pVanCC
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166982-plasmid-map-sequence-id-331335

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Showing: 11401 - 11440 of 32163 results