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pSLS.405

Plasmid

#187203

Purpose

Expresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA (wildtype)
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pQE-meGFP-DLC1

Plasmid

#192048

Purpose

Plasmid expresses DLC1 binding peptide to Talin R8 domain in chimera with meGFP

Depositor

Ionel Popa

Article

Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

Insert

DLC1 (DLC1 Human)

Expression

Bacterial

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-ATP

Plasmid

#225247

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-ATP

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP-Rab27a

Plasmid

#194985

Purpose

overexpress Rab27a

Depositor

Angelique Bordey

Insert

GFP-Rab27a

Expression

Bacterial

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pELlR4

Plasmid

#172545

Purpose

Inducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Carbenicillin Resistance Marker.

Depositor

Christopher Reisch

Insert

LacI-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLlacO-1

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMRE155

Plasmid

#118505

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHCKan-phoAp-GFPuv

Plasmid

#139072

Purpose

Low phosphate inducible promoter phoAp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

phoAp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

Plasmid

#195741

Purpose

Base editing for making a pre-stop codon in mmoX using phenol inducible system

Depositor

Seung-Goo Lee

Article

Jeong et al Chem Eng J, 2023

Insert

Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Po (phoenol-inducible promoter)

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pKL09-GCaMP6f-mScarlet bb118

Plasmid

#158979

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f-mScarlet fusion

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23118 (sigma 70)

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-DogTag Loop A

Plasmid

#171779

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop A

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Val2…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLP1

Plasmid

#209988

Purpose

Entry vector to store Level 0 parts

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLP2

Plasmid

#209989

Purpose

Entry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI

Plasmid

#115214

Purpose

Expresses PauCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PauCas13

Tags

SUMO

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET28-6His-TEV-Nsp14

Plasmid

#154954

Purpose

Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli

Depositor

Justin Kollman

Insert

6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)

Tags

6His-TEV

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLG017 (AVAST type 1)

Plasmid

#157895

Purpose

Expresses the AVAST type 1 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

AVAST type 1 (STAND ATPase with N-terminal serine protease)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLMB509

Plasmid

#40084

Depositor

Philip Poole

Article

Tett et al Appl Environ Microbiol. 2012 Oct;78(19):

Inserts

gfpmut 3.1
tauR
tau promoter

Expression

Bacterial

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pWJB9

Plasmid

#126650

Purpose

Expresses AdrA from a modified pLAFR3

Depositor

Ute Romling

Article

Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

Insert

adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30

Tags

8xHis

Expression

Bacterial

Promoter

PBAD

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-ART

Plasmid

#174810

Purpose

Bacterial expression of isolated PARP1 ART subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-ART (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-BRCT

Plasmid

#174807

Purpose

Bacterial expression of isolated PARP1 BRCT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-BRCT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-WGR

Plasmid

#174808

Purpose

Bacterial expression of isolated PARP1 WGR domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-WGR (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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