30,807 results

1. 6xHis-MBP-BrCas12b

   Plasmid

   #182276

   Purpose

   Expresses recombinant BrCas12b

   Depositor

   Piyush Jain

   Article

   Nguyen et al EBioMedicine. 2022 Mar;77:103926. doi: 1

   Insert

   BrCas12b
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, T7, Thrombin site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)

   Plasmid

   #156463

   Purpose

   Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM

   Depositor

   Andre Stiel

   Article

   Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

   Inserts

   Heme Oxygenase
   ReBphP-PCM
   

   Tags

   His Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6P1-DEST-His

   Plasmid

   #119763

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal 10xHis tag.

   Depositor

   Andrew Jackson, Martin Reijns

   Type

   Empty backbone

   Tags

   10xHis and GST

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPhlF-P3

   Plasmid

   #74687

   Purpose

   PhlF-P3 gate

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Insert

   PTac-RBSP3-PhlF-PPhlF-YFP
   

   Expression

   Bacterial

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD-Citroff1

   Plasmid

   #134301

   Purpose

   Bacterial expression of inverse-response citrate sensor with high binding affinity

   Depositor

   Robert Campbell

   Article

   Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

   Insert

   Citroff1
   

   Tags

   6-His tag

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcNB1

   Plasmid

   #89241

   Purpose

   Resurfaced Cell-penetrating GFP nanobody

   Depositor

   Brian McNaughton

   Article

   Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

   Insert

   pcNB1 (GFP)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQTEV-PTD004

   Plasmid

   #34798

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   GTP-binding protein PTD004 (OLA1 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET16b-MYPT1(1-314)

   Plasmid

   #224893

   Purpose

   Bacterial expression of MYPT1(1-314)

   Depositor

   Mathieu Bollen

   Article

   Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

   Insert

   myosin phosphatase targeting subunit 1 (PPP1R12A )
   

   Expression

   Bacterial

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pY2-MET-IV

   Plasmid

   #218117

   Purpose

   MET-HYGRO plasmid (218133) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pY2-LYS-IV

    Plasmid

    #218118

    Purpose

    LYS-Leu2 plasmid (218114) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBEL1284

    Plasmid

    #175960

    Purpose

    For expression of LetA-LetB operon from E. coli. N-terminal his tag on LetA, araBAD promoter

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Inserts

    LetA
    LetB
    

    Tags

    6xHis 2xQH and TEV cleavage site on LetA

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIneo-AR-S256A

    Plasmid

    #171227

    Purpose

    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser256Ala (AR-S256A)

    Depositor

    Steven Balk

    Article

    Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

    Insert

    human androgen receptor Ser256Ala mutant (AR Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    AR-Ser256Ala (AR-S256A)

    Promoter

    CMV

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIneo-AR-S308A

    Plasmid

    #171229

    Purpose

    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser308Ala (AR-S308A)

    Depositor

    Steven Balk

    Article

    Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

    Insert

    human androgen receptor Ser308Ala mutant (AR Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    AR-Ser308Ala (AR-S308A)

    Promoter

    CMV

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIneo-AR-S90A

    Plasmid

    #171225

    Purpose

    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser90Ala (AR-S90A)

    Depositor

    Steven Balk

    Article

    Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

    Insert

    human androgen receptor Ser90Ala mutant (AR Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    AR-Ser90Ala (AR-S90A)

    Promoter

    CMV

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAH69

    Plasmid

    #173782

    Purpose

    The pAH69 plasmid [Haldimann and Wanner 2001] is the HK022 helper plasmid necessary for genomic integration of the pIT5_KH plasmid.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    HK022 Integrase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-28a RUVBL2 Full Length

    Plasmid

    #159142

    Purpose

    Expresssion and protein purification of full length RUVBL2

    Depositor

    Eric Erquan Zhang

    Article

    Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

    Insert

    RUVBL2 (RUVBL2 Human)
    

    Expression

    Bacterial

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMPMA3dPlac-null-GFPLVA

    Plasmid

    #23338

    Depositor

    Olivia Steele-Mortimer

    Article

    Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

    Insert

    GFP(LVA)
    

    Tags

    GFP(LVA)

    Expression

    Bacterial

    Available Since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

18. ESCRT-I

    Plasmid

    #17634

    Depositor

    James Hurley

    Article

    Kostelansky et al Cell. 2006 Apr 7. 125(1):113-26.

    Insert

    Vps23(1-385) – His/Vps28(1-242) – Vps37(1-213) – Mvb12(1-101) (VPS28, STP22, SRN2 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. PJQ47

    Plasmid

    #134646

    Purpose

    Contains Promoter 1 with bsa1 cut sites for inserting sgRNA

    Depositor

    Jason Peters, Oren Rosenberg

    Article

    Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Anderson BBa_J23117

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. PJQ48

    Plasmid

    #134647

    Purpose

    Contains Promoter 2 with bsa1 cut sites for inserting sgRNA

    Depositor

    Jason Peters, Oren Rosenberg

    Article

    Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Anderson BBa_J23114

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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