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pLG022 (dsr1)

Plasmid

#157900

Purpose

Expresses the dsr1 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

dsr1 (SIR2-DUF4020)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET-2(KW)2-eGFP

Plasmid

#112736

Purpose

Express 2(KW)2-eGFP in E. coli

Depositor

Kyubong Jo

Article

Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

Insert

2(KW)2-eGFP

Tags

6xHis

Expression

Bacterial

Promoter

T7 Promoter

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBbE5a-Opto-Cre-Vvd

Plasmid

#134404

Purpose

Light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

Depositor

Mary Dunlop

Article

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

Opto-Cre-Vvd

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET_BGL3

Plasmid

#101912

Purpose

Expresses a β-glucosidase gene from Rhodothermus marinus in E. coli under the control of the pET system.

Depositor

Steve Shih

Article

Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944

Insert

β-glucosidase

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7+LacO

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAKlux1

Plasmid

#14073

Depositor

Attila Karsi

Article

Karsi et al FEMS Microbiol Lett. 2006 Jul. 260(2):21

Insert

Bacterial luciferase

Expression

Bacterial

Available Since

March 12, 2007

Availability

Academic Institutions and Nonprofits only
pGMCtKq28-TSC10M1-sspB

Plasmid

#49526

Purpose

Kanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

TSC10M1-sspB

Expression

Bacterial

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

Plasmid

#122084

Purpose

Express M.EcoGII fused to TRF1 with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII-V5-TERF1

Use

Retroviral

Tags

V5 tag on Nter of TERF1

Expression

Bacterial and Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-His6-Ran(Q69L)

Plasmid

#171563

Purpose

Encodes human Ran protein

Depositor

Yan Zeng

Insert

Ran (RAN Human)

Expression

Bacterial

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCheY Tc delta C

Plasmid

#13520

Depositor

James McKerrow

Article

Eakin et al J Biol Chem. 1993 Mar 25. 268(9):6115-8.

Insert

cruzain

Tags

CheY

Expression

Bacterial

Mutation

Truncated variant: stop codon after Gly212

Available Since

March 11, 2009

Availability

Academic Institutions and Nonprofits only
pJUMP29-1E(sfGFP)

Plasmid

#126982

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pYTK002

Plasmid

#65109

Purpose

Encodes ConLS as a Type 1 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

ConLS

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-WASP-GBD

Plasmid

#30113

Depositor

David Sacks

Article

Kim et al J Biol Chem. 2000 Nov 24. 275(47):36999-

Insert

WASP-GBD (WAS, WAS Human)

Tags

GST

Expression

Bacterial

Mutation

Contains the Cdc42-interacting domain of WASP (aa…

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT-MBI (JBEI-3085)

Plasmid

#87950

Purpose

the overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).

Depositor

Taek Soon Lee

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Insert

MevT-MBI

Expression

Bacterial

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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TRBV20-1

Plasmid

#123454

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV20-1 (TRBV20-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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XP7 Sonic hh

Plasmid

#16983

Depositor

Randall Moon

Insert

sonic hedgehog

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
etnppl-pGEMtEasy

Plasmid

#192263

Purpose

Mouse Etnppl sequence is inserted in pGEM-T Easy vector.

Depositor

Toshihide Yamashita

Article

Tsujioka et al Front Cell Neurosci. 2023 Jan 30;17:1097

Insert

ethanolamine phosphate phospholyase (Etnppl Mouse)

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pOTIII.EFGP.IGR3.6

Plasmid

#40866

Depositor

Harold Gainer

Article

Fields et al J Neurosci. 2003 Aug 27;23(21):7801-9.

Insert

MOUSE OXYTOCIN GENE (Oxt Mouse)

Expression

Bacterial

Promoter

mouse oxytocin promoter

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pMRBAD-link-CGFP

Plasmid

#52733

Purpose

L-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

link-CGFP

Expression

Bacterial

Promoter

PBAD

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pRM053 His-TEV-Cas9 triNLS 0C

Plasmid

#196244

Purpose

Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Article

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Use

CRISPR

Tags

HA, His6 (N terminal on backbone), NLS, Nucleopla…

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET22b-TEVP-cleavableELP

Plasmid

#219402

Purpose

Tobacco etch virus protease-responsive elastin-like polypeptide (ELP)

Depositor

Danielle Mai

Article

Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

Insert

TEVP-cleavableELP

Tags

6xHis

Expression

Bacterial

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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