30,296 results

1. pE2c

   Plasmid

   #17462

   Purpose

   modified pENTR vector for C-terminal 3x HA tag fusion with your gene of interest

   Depositor

   Giovanna Benvenuto

   Article

   Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

   Type

   Empty backbone

   Use

   Modified gateway entry vector

   Tags

   3xHA

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET11b_MutT

   Plasmid

   #73155

   Purpose

   Expresses in E. coli, E coli MutT

   Depositor

   Maurice Bessman, Sandra Gabelli

   Article

   Bessman et al J Biol Chem. 1996 Oct 11;271(41):25059-6

   Insert

   MutT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

3. Hela kinase-dead MLCK-GFP

   Plasmid

   #46317

   Depositor

   Anne Bresnick

   Article

   Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

   Insert

   HeLa long myosin light chain kinase-dead (MYLK Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   E1626K

   Promoter

   CMV

   Available since

   July 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pMRE-Tn5-140

   Plasmid

   #118524

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   TagBFP2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEK22

   Plasmid

   #210588

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   BdH3pro-Hshph-G4S-nanoluc-ScADH1ter
   

   Use

   Luciferase

   Tags

   NanoLuc luciferase

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   BdH3pro

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. YWHAE

   Plasmid

   #39146

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   YWHAE (YWHAE Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pQTEV-SCP2

   Plasmid

   #31336

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   sterol carrier protein 2 (SCP2 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSALECTNK-TEM1(S70A,D179G)/csTEM1

   Plasmid

   #81163

   Purpose

   pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1

   Depositor

   Timothy Whitehead

   Article

   Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

   Insert

   TEM-1 beta-lactamase
   

   Use

   Tags

   Codon swapped TEM-1 from H26 to W290 and His6x

   Expression

   Bacterial

   Mutation

   Deleted the sec transport tag. Plasmid encodes H2…

   Promoter

   lac promoter

   Available since

   Feb. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPINB-hRNF216(457-784)

   Plasmid

   #171922

   Purpose

   Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic helix-RBR-helix construct suitable for activity assays.

   Depositor

   Bernhard Lechtenberg

   Article

   Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

   Insert

   E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
   

   Use

   Tags

   3C protease cleavage site and 6x-His tag

   Expression

   Bacterial

   Mutation

   codon optimised for expression in E. coli

   Promoter

   T7

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE-80L iLID (C530M)

    Plasmid

    #60408

    Purpose

    E. coli expression of iLID (C530M)

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    iLID C530M
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C530M

    Promoter

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

11. Human HUWE1 (3993-4374)

    Plasmid

    #110754

    Purpose

    Bacterial Expression of HUWE1 HECT domain

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-4T2-14-3-3 tau GST

    Plasmid

    #13281

    Depositor

    Michael Yaffe

    Article

    Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

    Insert

    14-3-3 tao (YWHAQ Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pET28a-RILP

    Plasmid

    #74600

    Purpose

    used for expression in E. coli and purification

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab-interacting lysosomal protein (RILP Human)
    

    Use

    Tags

    FLAG, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-Cre-6xHis

    Plasmid

    #62939

    Purpose

    Expression of Cre-6xHis in bacterial of cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cre recombinase
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSEVA221rAPOBEC1-T7RNAP

    Plasmid

    #167979

    Purpose

    Expresses fusion protein rAPOBEC1-T7RNAP in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

    Insert

    rAPOBEC1-T7RNAP (T7p07 Synthetic)
    

    Use

    Tags

    T7 tag and T7RNAP

    Expression

    Bacterial

    Mutation

    Promoter

    TetR-Ptet

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJKR-H-araC

    Plasmid

    #62563

    Purpose

    Arabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET tdTomato LIC cloning vector (u-tdTomato)

    Plasmid

    #86926

    Purpose

    pET LIC cloning vector for yORF-tdTomato

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    tdTomato

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAT03-Apr

    Plasmid

    #154756

    Purpose

    For curing of the resistance marker of RecET recombinase system in Acinetobacter baumannii, Aprar

    Depositor

    Sheng Yang

    Article

    Sun et al mSphere. 2020 Apr 1;5(2). pii: 5/2/e0089

    Insert

    FLP recombinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. 5KO9 (HMCES)

    Plasmid

    #101877

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HMCES (HMCES Human)
    

    Use

    Tags

    6x His (modified C-term tag is AENLYFQSHHHHHH)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pRibo EcoHind

    Plasmid

    #36252

    Depositor

    Carolyn Bertozzi, Jessica Seeliger

    Article

    Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

    Type

    Empty backbone

    Use

    Synthetic Biology; Mycobacteria replicating vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Modified Hsp60

    Available since

    April 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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