32,826 results
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Plasmid#157872Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal IDRDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only
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pCDF-NtXNtS1R1AtR2NtB2
Plasmid#160881PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
ExpressionBacterialPromoterrbcS-S1: T7, chaperones: T7Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
41 pCOLI_K_NoTag
Plasmid#160265PurposeUntagged Kan resistant bacterial expression vectorDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
40 pCOLI_K_MBP
Plasmid#160183PurposeN-term MBP 3C cleavable Kan resistant bacterial expression vectorDepositorTypeEmpty backboneTags6xHis and MBPExpressionBacterialPromoterT7Available SinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-OspC
Plasmid#159998PurposeExpresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cellsDepositorInsertSpyTag003-OspC (20-209) C129S
TagsHis6-tag and Thrombin cleavage siteExpressionBacterialMutationC129S mutation improves expression level.PromoterT7Available SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
64 pCOLI_G2
Plasmid#160206PurposeGentamycin resistant polycistronic host vectorDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CAPNS1_EF-hand_8+EF-hand_5
Plasmid#109995PurposeProtein expression and purification of CAPNS1_EF-hand_8+EF-hand_5DepositorInsertCAPNS1_EF-hand_8+EF-hand_5 (CAPNS1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_VDR_Hormone-recep
Plasmid#109962PurposeProtein expression and purification of VDR_Hormone-recepDepositorInsertVDR_Hormone-recep
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PCMT1_PCMT
Plasmid#109932PurposeProtein expression and purification of PCMT1_PCMTDepositorInsertPCMT1_PCMT
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_YWHAE_14-3-3
Plasmid#109942PurposeProtein expression and purification of YWHAE_14-3-3DepositorInsertYWHAE_14-3-3
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESRRB_Hormone-recep
Plasmid#109909PurposeProtein expression and purification of ESRRB_Hormone-recepDepositorInsertESRRB_Hormone-recep (ESRRB Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
T7 promoter + rpsL reporter plasmid
Plasmid#156455PurposeA mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.DepositorInsertsrpsL
TcR
ExpressionBacterialMutationATG start codon mutated to ACGPromotertac and tetAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2774
Plasmid#160667PurposeMobile CRISPRi sfGFP 'test', gmc6 sgRNADepositorInsertgmc6 (gfp) sgRNA
ExpressionBacterialAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
T7pr-His6-MBP-TEV-dCas9-BirA*
Plasmid#159994PurposeBacterial expression of dCas9-BirA*DepositorInsertdCas9-BirA*
Tags6X-His, MBP, 3X-FLAG, NLSExpressionBacterialMutationD10A, H840APromoterT7Available SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSHDY-PvanCC-mVenus_vanR
Plasmid#137664PurposeConjugative vector derived from pSHDY. Constitutive expression of the repressor vanR, as well as vanillate-inducible expression of mVenus.DepositorInsertPvanCC:mVenus
ExpressionBacterialPromoterPvanCCAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNusGFL
Plasmid#113122PurposeExpresses full-length E. coli NusGDepositorAvailable SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
p40phox-PX (1-144)
Plasmid#119123PurposeBacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)DepositorInsertp40phox-PX (1-144)
TagsGSTExpressionBacterialPromotertacAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pV2A-T
Plasmid#153022PurposeSingle-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoterDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
poGFP1
Plasmid#154127PurposeExpresses orthogonal gfp gene in bacterial cellsDepositorInsertorthogonal gfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
HS_NAM-B1
Plasmid#154064PurposeLevel M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosTDepositorInsertsHygromycin phosphotransferase
Cre recombinase
LoxP-flanked GUS and TtNAM-B1
UseCre/Lox and Synthetic Biology; Golden gateExpressionBacterial and PlantMutationThe TtNAM-B1 gene sequence was domesticated to re…PromoterHvHSP17, OsAct, and ZmUbiAvailable SinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X09-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D
Plasmid#159541PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) G12D (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG12DAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
EC71173
Plasmid#154061PurposeLevel 1, Position 2 Golden Gate vector. HspHv17::Cre-U5-CreDepositorInsertCre recombinase
ExpressionBacterialMutationIntroduction of U5 intron sequences, to prevent p…PromoterHvHSP17Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
LaM8-GG15
Plasmid#159593PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at GG15
ExpressionBacterialAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT D554N
Plasmid#154279PurposeBacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationAspartate 554 changed to Asparagine- bacterial co…Available SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-gSTEP1
Plasmid#159376PurposeExpresses gSTEP1 in E. coliDepositorInsertgSTEP1
Tags6x His tagExpressionBacterialPromoterT7Available SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11a-STEPtag
Plasmid#159373PurposeExpresses STEPtag in E. coliDepositorInsertSTEPtag
Tags6x His tagExpressionBacterialPromoterT7Available SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
gNS2B47NS3 S135A
Plasmid#155317PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationS135APromoterM13Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ2072.2_c1
Plasmid#140675PurposeCRISPRlator control (“open ring”)DepositorInsertCRISPRlator control (“open ring”)
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a RUVBL2 Full Length
Plasmid#159142PurposeExpresssion and protein purification of full length RUVBL2DepositorInsertRUVBL2 (RUVBL2 Human)
ExpressionBacterialAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-IS186
Plasmid#140627PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186
UseCRISPR; TransposonExpressionBacterialAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only