32,167 results
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Plasmid#164869PurposeThe pCYMR-1.phiKO2cB plasmid carrying the gene for phiKO2 cB repressor. Genomic integration vector for expression of any candidate DNA looping protein.DepositorInsertphiKO2 cB repressor
ExpressionBacterialAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM cCol2 (CT#617)
Plasmid#13943DepositorAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
p2T-CAG-KKHSaCas9-BlastR
Plasmid#107189PurposeConfers constitutive expression of KKH SaCas9.DepositorInsertKKH SaCas9 (NEWENTRY )
ExpressionBacterialMutationE782K/N968K/R1015HPromoterChicken ß-ActinAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEPR
Plasmid#199233PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProepiregulin preproprotein (EREG Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB232
Plasmid#170019PurposeEncodes LacI-controlled expression of surface-displayed murine CRAMP and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CRAMP
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TXN_Thioredoxin
Plasmid#109778PurposeProtein expression and purification of TXN_ThioredoxinDepositorInsertTXN_Thioredoxin (TXN Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCON1-ProB.gfp
Plasmid#112516PurposeProB driven gfpmut3 expression with synTTDepositorInsertProB gfpmut3 TT
ExpressionBacterialPromoterProBAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS26
Plasmid#32295DepositorTypeEmpty backboneUseBacterial gene additionExpressionBacterialPromoterrhaBAvailable SinceSept. 26, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX5X.1 PINK1 KD
Plasmid#13322DepositorInsertPINK1 kinase domain (PINK1 Human)
TagsGSTExpressionBacterialMutationAmino acids 112-496; Kinase dead: K219D/D362A/D3…Available SinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
CFL1 (CFL1A-c002)
Plasmid#98237PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCFL1A (CFL1 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00241:Cofilin_ADF 27-148PromoterT7Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC002 - LshC2C2 locus into pACYC184
Plasmid#79151PurposeEndogenous LshC2c2 locus cloned into pACYC184DepositorInsertLshC2c2 locus
ExpressionBacterialAvailable SinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pST39-Hrs-STAM1 core
Plasmid#21497DepositorTagsHisExpressionBacterialMutationnoneAvailable SinceFeb. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKM263-USP7
Plasmid#196365Purposeprotein expression of the GST-tagged catalytic domainDepositorInsertUSP7 catalytic domain
TagsHis6-GST-TEVExpressionBacterialMutationcontains only S207-E560PromoterT7Available SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHG5
Plasmid#188253PurposeIntegrating plasmid contains the SV1 int/attP locus and the kanamycin-resistance gene (aphII) for selectionDepositorTypeEmpty backboneExpressionBacterialPromoteract1p/actII-orf4Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTBXh-2
Plasmid#26078DepositorTypeEmpty backboneTagsHisExpressionBacterialAvailable SinceNov. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAL-pck_NT
Plasmid#74069PurposepAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecRDepositorInsertsgRNA pck (NT)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterptacAvailable SinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBluescript GPX-1
Plasmid#13038DepositorInsertGPX-1 (GPX1 Human)
ExpressionBacterialMutationAdditional GCG upstream of codon 11. Codon 92 is …Available SinceNov. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRW004-SM-destination-proUBQ10-Cas9
Plasmid#104438PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRExpressionBacterial and PlantPromoterUBQ10Available SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINMSYB
Plasmid#41118DepositorTypeEmpty backboneExpressionBacterial, Insect, and Mamm…Available SinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPro24
Plasmid#17805DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceApril 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
SNX29-PX (269-391)
Plasmid#119108PurposeBacterial expression of human phox homology (PX) domain, SNX29-PX (269-391)DepositorInsertSNX29-PX (269-391)
TagsGSTExpressionBacterialPromotertacAvailable SinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-BmNG
Plasmid#232207PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-BSD-3xTyDepositorInsertmNeongreen
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-PmNG
Plasmid#232201PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTyDepositorInsertmNeongreen
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEM8315
Plasmid#213988PurposePrpsM driven expression of mCherry2-LDepositorInsertmCherry2-L
ExpressionBacterialPromoterPrpsMAvailable SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1_CylLS_CylM
Plasmid#208760PurposeExpresses modified cytolysin S (mCylLS) in E. coliDepositorInsertsCylLS
CylLM
TagsHisx6 TagExpressionBacterialPromoterT7Available SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PXWABS62(E11-TEV)
Plasmid#123157PurposeFor TEV-based leakiness regulation. pSB4A3 carrying ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-tDepositorInsertParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t
UseSynthetic BiologyExpressionBacterialPromoterParsR-ABS62, Pecf11Available SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5238
Plasmid#128711PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopG1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSG291
Plasmid#115605Purposesurface-displayed Null, used in composability experiments; low copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-OspC
Plasmid#159998PurposeExpresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cellsDepositorInsertSpyTag003-OspC (20-209) C129S
TagsHis6-tag and Thrombin cleavage siteExpressionBacterialMutationC129S mutation improves expression level.PromoterT7Available SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSN007
Plasmid#102440PurposeFor PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)DepositorInsert7SK paired gRNA insert
UseSynthetic BiologyExpressionBacterialAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only