32,826 results
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Plasmid#115210PurposeExpresses AspCas13b from a SUMO-tagged bacterial expression constructDepositorInsertAspCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKEW321-MbCas12a-RFP-GFP-array
Plasmid#115661PurposeEncodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1DepositorInsertGFP and RFP array for MbCas12
UseCRISPRExpressionBacterialPromoterPlacAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG216
Plasmid#114002PurposeLevel 2 golden gate cloning vector for medium copy number free-living expressionDepositorTypeEmpty backboneExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI
Plasmid#115207PurposeExpresses BzoCas13b from a SUMO-tagged bacterial expression constructDepositorInsertBzoCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPS0379
Plasmid#115510PurposeSuitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFPDepositorInsertRlv3841pNifH(pOGG082) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPS0314
Plasmid#115507PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celBDepositorInsertcelB pOGG050, T-pharma pOGG003 assembled in pOGG026
ExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterConstitutive promoter pNeo pOGG001Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPS0359
Plasmid#115508PurposeReporter Plasmid. Taurine-inducible expression of GFPDepositorInsertsfGFP pOGG037, T-pharma pOGG003 assembled in pOGG024
ExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterTaurine-inducible promoter of S. meliloti Promote…Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a His tag-FRB-Venus C
Plasmid#100980PurposeEncodes half of a protein delivery sensor (His-FRB-Venus C) for expression in and purification from BacteriaDepositorInsertHis-FRB-Venus C
TagsHis tagExpressionBacterialPromoterT7Available SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_39
Plasmid#109238PurposePTac driving TALEsp1DepositorInsertTALEsp1
ExpressionBacterialPromoterPTacAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gma-pCAMBIA-1301-STTM171
Plasmid#84080PurposeDisturb the expression of miR171 in soybeanDepositorInsertmiR171
ExpressionBacterial, Plant, and Yeast…Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRC01
Plasmid#114680PurposesfGFP.Strands 1-9DepositorInsertsfGFP Strands 1-9
TagsGSTExpressionBacterialPromoterT7Available SinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
B6-3
Plasmid#111487PurposeSer-tRNA synthetaseDepositorInsertSer-tRNA synthetase
Tags6xHisExpressionBacterialAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B6-1
Plasmid#111485PurposePhe-tRNA synthetase BDepositorInsertPhe-tRNA synthetase B
Tags6xHisExpressionBacterialAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B5-4
Plasmid#111483PurposeTrp-tRNA synthetaseDepositorInsertTrp-tRNA synthetase
Tags5xHisExpressionBacterialAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B6-4
Plasmid#111488PurposePhe-tRNA synthetase ADepositorInsertPhe-tRNA synthetase A
Tags6xHisExpressionBacterialMutationA81SAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDB328
Plasmid#113672Purposeexpresses Ulp1_R4 in Escherichia coliDepositorInsertUlp1_R4
Tags6-HisExpressionBacterialMutationcontains solubility enhancing mutationsPromoterT7Available SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP
Plasmid#112631PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral RGDSP integrin-binding sequencePromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
DLG4_PDZ_3
Plasmid#103953PurposeProtein expression and purification of PSD95 PDZ3 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
TIAM1_PDZ_1
Plasmid#103925PurposeProtein expression and purification of TIAM PDZ domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
DLG1_PDZ_3
Plasmid#103917PurposeProtein expression and purification of DLG1 PDZ3 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4L WW domain #1
Plasmid#104204PurposeBacterial expression of WW domain #1 from NEDD4LDepositorInsertNEDD4L WW domain #1 (NEDD4L Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4L WW domain #4
Plasmid#104207PurposeBacterial expression of WW domain #4 from NEDD4LDepositorInsertNEDD4L WW domain #4 (NEDD4L Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 C20orf67 WW domain
Plasmid#104273PurposeBacterial expression of WW domain from C20orf67DepositorInsertC20orf67 WW domain (PCIF1 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP5GP
Plasmid#112610PurposeUsed for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneTags8His-GST and AviExpressionBacterialAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP5BT
Plasmid#112609PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneTags8His-GB1 and AviExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAT11
Plasmid#112592PurposeUsed for expression of protein in E. coli as Thrombin and TEV cleavableN-terminal 6His-tag-MBP fusions and without NcoI site within MBP.DepositorTypeEmpty backboneTags6His-MBPExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pANY6
Plasmid#112198PurposeThis plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.DepositorTypeEmpty backboneTagsELP-tagExpressionBacterialAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin (1-76; T66V, L67N)
Plasmid#110755PurposeBacterial expression of human Ubiquitin (1-76; T66V, L67N)DepositorAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB076i6
Plasmid#79200PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (no operator) gIII, luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (no operator); Ppro1Available SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW204
Plasmid#113244PurposeE. coli expression vector for IHFa F13A + IHFbDepositorAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only