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32,167 results

Showing: 8911 - 8940 of 32167 results

  1. pMARO

    Plasmid
    #178086
    Purpose
    Derivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.
    Depositor
    Sören Abel
    Article
    Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10
    Insert
    TcR (tetA/tetR)
    Expression
    Bacterial
    Mutation
    Tetracycline resistance cassette is flanked by Ec…
    Available Since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    178086-plasmid-map-sequence-id-349998

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  3. pHis-TEV-Nsp15

    Plasmid
    #187656
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004
    Insert
    Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7lac
    Available Since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    187656-plasmid-map-sequence-id-388768

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  4. pDB038a

    Plasmid
    #99211
    Purpose
    Accessory plasmid for PACE of PylRS variants
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT
    Expression
    Bacterial
    Mutation
    amber codons at positions 29 and 184 of gIII
    Promoter
    Ppsp and PproK
    Available Since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99211-plasmid-map-sequence-id-192359

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  5. STK25

    Plasmid
    #39075
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    STK25 (STK25 Human)
    Tags
    His6-Zb-TEV
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. pETM6-4F2-mCherry

    Plasmid
    #73421
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P4F2 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73421-plasmid-map-sequence-id-135321

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  21. pGBW-m4136458

    Plasmid
    #149531
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149531-plasmid-map-sequence-id-297078

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  29. pET15b LFn-Fla 3A

    Plasmid
    #84872
    Purpose
    Bacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    Fla
    Tags
    His
    Expression
    Bacterial
    Mutation
    3 C-terminal leucines have been replaced by alani…
    Promoter
    T7
    Available Since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  30. pTriEx-PA-Rac1-C450A

    Plasmid
    #22025
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
    Tags
    6X His
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV domain contains light-insensitive mutation C4…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
Showing: 8911 - 8940 of 32167 results