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Plasmid#208931PurposeRecognition domain for in vitro MAPK-activity sensorDepositorInsertWW domain of PIN1 (PIN1 Human)
UseTagsHISx6, LgBit, and MBPExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTCF120 H-2D(b)
Plasmid#180454PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2D(b)
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAI1_G-alpha
Plasmid#110012PurposeProtein expression and purification of GNAI1_G-alphaDepositorInsertGNAI1_G-alpha (GNAI1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM005
Plasmid#170009PurposeEncodes PvirK-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PvirKAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-D-TOPO-PARP14-SΔK
Plasmid#216658PurposeExpresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.DepositorInsertPARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
UseTags6xHis-V5-TEVExpressionBacterialMutationDeletion starting from Q1390 to D1448 and substit…PromoterT7Available sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57_beta-globin-5'UTR_Nanoluc
Plasmid#219989Purposebetaglobin-5'UTR-NanoLuc mRNA synthesisDepositorInsertbetaglobin-5'UTR-NanoLuc (HBB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETTEV-SENP8
Plasmid#196361Purposeprotein expression of the His-tagged full length proteinDepositorInsertSENP8/NEDP1
UseTagsHis6-TEVExpressionBacterialMutationPromoterT7Available sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP46-2A(sfGFP)
Plasmid#126990PurposeLevel 2 vector. sfGFP reporter. Origin p15A (medium copy number)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
WARS_pNIC-CTB10H
Plasmid#153058PurposeExpression construct for producing Avi tagged Tryptophanyl-tRNA synthetaseDepositorInsertWARS (WARS1 Human)
UseTagsTEV-Avi-His10ExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJB166
Plasmid#86998PurposeCRISPR/Cas9 in fission yeast using fluoride selectionDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisD-rsTagRFP
Plasmid#31927DepositorInsertrsTagRFP
UseTagsHisExpressionBacterialMutationF84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…PromoteraraBAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cut-P(11)4
Plasmid#185785PurposeEncodes enzyme cutinase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertCut-P(11)4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD33.1-DpnII
Plasmid#127413PurposeExpresses DpnII restriction endonuclease in bacteriaDepositorInsertDpnII
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
csr-1_hH1_mEos3.1_V5
Plasmid#191750PurposePhotoconvertible fluorescent nuclear reporter.DepositorInserthH1 (hH1 Neurospora crassa)
UseTagsV5, ccg-1 promoter, and mEos3.1ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
4Z3C (TET3)
Plasmid#119969PurposeBacterial expression for structure determinationDepositorInsertTET3 (TET3 Human)
UseTags6xHis-TEVExpressionBacterialMutationcontains amino acids 48-98PromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMALcPP-MS2cp
Plasmid#203351PurposeExpresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteriaDepositorInsertMS2 coat protein
UseTagsHx6-MBPExpressionBacterialMutationV75E and A81G, to prevent capsid assemblyPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1∆HisTag_Nef_MCS2
Plasmid#66079PurposeSingle vector system expressing NEF allowing myristoylation of recombinant protein produce in Ecoli systemDepositorInsertNef
UseTags6xHis and HRV 3C SiteExpressionBacterialMutationPromoterT7-lacOAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_RRM2
Plasmid#157866Purposeexpress His tagged hnRNPA2 RRM2DepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
saposin B^W
Plasmid#161759PurposeSaposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophanDepositorInsertsaposin B R38_W
UseTagsHis-tagExpressionBacterialMutationchanged Arginine 38 to TryptophanPromoterT7Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -