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32,826 results

Showing: 9001 - 9030 of 32826 results

  3. pDC183

    Plasmid
    #106415
    Purpose
    Co-expresses trans SNARE complex with pQZ210 in E. coli
    Depositor
    Axel Brunger
    Article
    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d
    Inserts
    SNAP25 fragment 141-204 (Snap25 Rat, Synthetic)
    SNAP25 Fragment 7-83 (Snap25 Rat, Synthetic)
    Expression
    Bacterial
    Mutation
    Fragment 141-204, Codon optimization and Fragment…
    Promoter
    T7
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106415-plasmid-map-sequence-id-204169

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  14. pHH0103 NEDD4 WW domain #4

    Plasmid
    #104211
    Purpose
    Bacterial expression of WW domain #4 from NEDD4
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4 WW domain #4 (NEDD4 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104211-plasmid-map-sequence-id-201499

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  18. pTO_Snf7_LAP

    Plasmid
    #101851
    Purpose
    Tet-inducible expression of Snf7 from S. cerevisiae with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    SNF7 (SNF7 Budding Yeast)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101851-plasmid-map-sequence-id-200251

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  21. pZH517

    Plasmid
    #102670
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102670-plasmid-map-sequence-id-199582

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  22. pZH512

    Plasmid
    #102666
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102666-plasmid-map-sequence-id-199387

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  28. 5TTW (EED)

    Plasmid
    #101254
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EED (EED Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 30, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101254-plasmid-map-sequence-id-197525

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Showing: 9001 - 9030 of 32826 results