30,307 results

1. pMAL-EFSep

   Plasmid

   #34627

   Depositor

   Dieter Söll, Jesse Rinehart

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Insert

   EF-Sep (tufB E. coli)
   

   Use

   Tags

   6xHIS and MBP

   Expression

   Bacterial

   Mutation

   His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

   Promoter

   Available since

   March 1, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pET24a_SMOXdel

   Plasmid

   #186785

   Purpose

   C-terminal His-Tag and a TEV cleaveage site, deletion of 8 residues (503-512) to transform into E. coli BL21 (DE3) cells

   Depositor

   Sandra Gabelli

   Article

   Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

   Insert

   Spermine oxidase (SMOX Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 503-512

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNIC-HIS6-NUSA-TEV-GG

   Plasmid

   #215798

   Purpose

   Cloning of target gene with N terminal His6 NUSA tag and TEV cleavage

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pHelper_TetR/pTet-Tnpase (carbR)

   Plasmid

   #213911

   Purpose

   ATc-inducible himar1C9 transposase expression - carb/amp resistance

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   TetR/ptet ATc-inducible himar1C9 transposase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDonor_Tn[sfGFP]

   Plasmid

   #213902

   Purpose

   Mariner transposon expressing sfGFP with kan selection casette

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with kan resistance and superfolder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDH-EF1a-HygroR-T2A-TetOn3G

   Plasmid

   #179887

   Purpose

   Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   TetOn3G
   

   Use

   Lentiviral

   Tags

   Hygromycin resistance

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. H6hSDHA

   Plasmid

   #113045

   Purpose

   Hetero expression of His-tagged human SDHA

   Depositor

   Edward A. Berry

   Article

   Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

   Insert

   H6hSDHA (SDHA Human)
   

   Use

   Tags

   His6 (MGSSHHHHHHSQDP-)

   Expression

   Bacterial

   Mutation

   Insert begins at amino acid 43

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMEXC3H

   Plasmid

   #110080

   Purpose

   Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3C cleavage site - His10 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-SpyCatcher002-MBP

   Plasmid

   #216295

   Purpose

   Expresses SpyCatcher002 linked to maltose-binding protein in bacterial cells

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SpyCatcher002-MBP
   

   Use

   Tags

   His6 tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCasX1

    Plasmid

    #87685

    Purpose

    p15A plasmid expressing CasX locus from Deltaproteobacteria

    Depositor

    Jennifer Doudna

    Article

    Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

    Insert

    CasX1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PGEX3X-GST-PTEN

    Plasmid

    #20743

    Depositor

    Alonzo Ross

    Article

    Campbell et al J Biol Chem. 2003 Sep 5. 278(36):33617-2

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

12. SpyCatcher002-oPent

    Plasmid

    #124664

    Purpose

    Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oPent
    

    Use

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEM cCol2 (CT#617)

    Plasmid

    #13943

    Depositor

    Cliff Tabin

    Article

    Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

    Insert

    Collagen II (COL2A1 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides 182-616

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pRSF-FGE

    Plasmid

    #102615

    Purpose

    Inducible expression of Formylglycine-generating enzyme

    Depositor

    Marc Wold

    Article

    Bain et al Methods Enzymol. 2018;600:439-461. doi:

    Insert

    Formylglycine-generating enzyme
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-tdMCP-12xGCN4

    Plasmid

    #205158

    Purpose

    express tdMCP-12xGCN4

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    tdMCP-12xGCN4
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH6HTC_minus_cer-region

    Plasmid

    #175297

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Type

    Empty backbone

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-N2H-N1

    Plasmid

    #125547

    Purpose

    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment1 attached to n-terminus of Gatew…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmRE-Tn5-147

    Plasmid

    #118526

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX TIF1gamma

    Plasmid

    #15734

    Depositor

    Joan Massague

    Article

    He et al Cell. 2006 Jun 2. 125(5):929-41.

    Insert

    TIF1 gamma (TRIM33 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pIBA2-Int-SpyTag

    Plasmid

    #198038

    Purpose

    Expression plasmid for producing SpyTagged stalled secretion variant of intimin

    Depositor

    Jack Leo

    Article

    Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

    Insert

    eaeA
    

    Use

    Tags

    SpyTag

    Expression

    Bacterial

    Mutation

    lacks native signal peptide (amino acids 1-39)

    Promoter

    tet

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge