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Bacterial Expression

30,535 results

Showing: 621 - 640 of 30535 results

  1. pSLTS

    Plasmid
    #59386
    Purpose
    Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.
    Depositor
    Shelley Copley
    Article
    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.
    Type
    Empty backbone
    Use
    Genome engineering
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59386-plasmid-map-sequence-id-380750

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  3. pBbB8k-csg-NbGFP

    Plasmid
    #166858
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469
    Insert
    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166858-plasmid-map-sequence-id-329027

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  4. pET-21b(+)-Is-PETase-W185A

    Plasmid
    #112204
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W185A
    Promoter
    N/A
    Available Since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112204-plasmid-map-sequence-id-215853

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  6. pZP06C

    Plasmid
    #232322
    Purpose
    pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.
    Depositor
    Chenggang Wu
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rpsJ
    Available Since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232322-plasmid-map-sequence-id-462278

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  7. pdCRISPomyces-2BD

    Plasmid
    #234429
    Purpose
    Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified dCas9(D10A, H840A) with polyaspartate linking
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Promoter
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234429-plasmid-map-sequence-id-468849

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  14. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid
    #85165
    Purpose
    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    Use
    Tags
    AmilCP and RTX
    Expression
    Bacterial
    Mutation
    Promoter
    J23100 promoter
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    85165-plasmid-map-sequence-id-354765

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Showing: 621 - 640 of 30535 results