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pRSETB/mStayGold(c4)

Plasmid

#212018

Purpose

For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

Depositor

Atsushi Miyawaki

Publication

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Type

Empty backbone

Tags

6xHis, T7 tag, Xpress tag, mStayGold(c4)

Expression

Bacterial

Promoter

T7

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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1GFP/RNase H1 WT

Plasmid

#174447

Purpose

Express GFP-tagged RNase H1 WT in E. coli.

Depositor

Karlene Cimprich

Publication

Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

RNase H1 (RNASEH1 Human)

Tags

His-tag, GFP

Expression

Bacterial

Promoter

T7 promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKAR2-Br512

Plasmid

#161875

Purpose

Expresses Br512 polymerase in E.coli

Depositor

Andrew Ellington

Publication

Maranhao et al medRxiv

Insert

Modified DNA Polymerase I Large Fragment (Br512)

Tags

8x His tag; Villin Headpiece 47 amino acid (HP47)…

Expression

Bacterial

Promoter

T7, tetPA

Available since

Nov. 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pET29b-IPP1-His

Plasmid

#124137

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Inorganic pyrophosphatase (IPP1 Budding Yeast)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET24b-APEX2

Plasmid

#111702

Purpose

For IPTG-inducible expression of APEX2 in E. coli

Depositor

Jessica Seeliger

Publication

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7 promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pACYC-Taq

Plasmid

#102793

Purpose

Expresses Taq DNA polymerase under WT T7 promoter

Depositor

Andrew Ellington

Publication

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Expression

Bacterial

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET-CjCas9

Plasmid

#89754

Purpose

Expression of CjCas9 with His tag in E.coli

Depositor

Seokjoong Kim

Publication

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

CjCas9

Use

CRISPR

Tags

6XHis, HA, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pORTMAGE-3

Plasmid

#72678

Purpose

Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.

Depositor

Csaba Pál

Publication

Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

Insert

mutL E32K

Tags

none

Expression

Bacterial

Mutation

E32K mutation conferring dominant mutator phenoty…

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pET28a-mCherry-CNA35

Plasmid

#61607

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with mCherry

Depositor

Maarten Merkx

Publication

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

mCherry and CNA35

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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HeLa full-length MLCK-GFP

Plasmid

#46316

Depositor

Anne Bresnick

Publication

Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

Insert

HeLa long myosin light chain kinase (MYLK Human)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET MBP GFP LIC cloning vector (MBP-msfGFP)

Plasmid

#29750

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), MBP, SiriusGFP, and TEV c…

Expression

Bacterial

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOPINF

Plasmid

#26042

Depositor

Ray Owens

Publication

Berrow et al Nucleic Acids Res. 2007. 35(6):e45.

Insert

His3C-lacZ

Tags

MAHHHHHHSSGLEVL

Expression

Bacterial, Insect, and Mamm…

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pTP223

Plasmid

#13263

Depositor

Kenan Murphy

Publication

Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

Insert

Plac-gam-bet-exo

Expression

Bacterial

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEX-4T2-14-3-3 tau GST

Plasmid

#13281

Depositor

Michael Yaffe

Publication

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 tao (YWHAQ Human)

Tags

GST

Expression

Bacterial

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pAWP78-PVCpnf1-16

Plasmid

#198271

Purpose

Unmodified pPVC; contains WT PVC structural/accessory genes

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf1-16

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

Plasmid

#190181

Purpose

Fluorescent reporter vector to visualize all of cell cycle phases.

Depositor

Toshiro Sato

Publication

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

iRFP
hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
TagBFP2
hGeminin(1/110)

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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SpyTag003-mKate2

Plasmid

#133452

Purpose

Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mKate2

Tags

GSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET_RTX_(exo-)

Plasmid

#102786

Purpose

Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

Depositor

Andrew Ellington

Publication

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase with 3'-5' deficient exonuclease

Expression

Bacterial

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKM444

Plasmid

#108319

Purpose

For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Inserts

Che9C RecT
Bxb1 Integrase
Tet Repressor

Expression

Bacterial

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pSNAP-tag (T7) Vector

Plasmid

#101137

Purpose

Expression of protein of interest with SNAP-Tag in bacterial cells

Depositor

New England Biolabs, Ana Egana

Type

Empty backbone

Tags

SNAP-tag (SNAP26b)

Expression

Bacterial

Available since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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