32,167 results
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Plasmid#102667PurposeConstitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
HTRA1_PDZ_1
Plasmid#103912PurposeProtein expression and purification of HTRA1 PDZ domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWA PAS-E BAD K-GAFm
Plasmid#39866DepositorInsertsiRFP protein PAS domain
iRFP protein GAF domain (mutated)
TagsE coil and K coilExpressionBacterialMutationF165Y, D232Y, W308RPromoterarabinose inducible and rhamnose inducibleAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
Ana GvpC-SpyTag-His in pET28a
Plasmid#85737PurposeExpresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coliDepositorInsertGas vesicle protein C (fused to SpyTag and His-tagged)
TagsHis-tag and SpyTagExpressionBacterialMutationAdded an extra glycine after the methionine at th…PromoterT7Available SinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKV NS5
Plasmid#145348PurposeCell-free/ mammalian expression vector for Zika virus NS5 protein with N-term eGFPDepositorInsertZIKV NS5
Tags8xHis and eGFPExpressionBacterialPromoterT7- CMV/CAGAvailable SinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
FtsZ
Plasmid#74128PurposeExpresses FtsZ without a peptideDepositorInsertFtsZ
TagsC-terminal linker sequence (G G S G S G S S)ExpressionBacterialPromoterT5-lacAvailable SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL 246
Plasmid#53014PurposeAmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1DepositorInsertGFP
UseSynthetic BiologyTagsfimS (off orientation)ExpressionBacterialAvailable SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAN-NOR
Plasmid#62306Purposesynthetic circuit: NOR gateDepositorInsertsA2NT sgRNA
A2NT sgRNA
UseCRISPRExpressionBacterialPromoterPPhlF and pBADAvailable SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046916
Plasmid#145627PurposeBacterial Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-His6-SUMO-cSrc-SH2-AviTag
Plasmid#214230PurposeBacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selectionsDepositorInsertcSrc kinase SH2 domain (SRC Human)
Tags6xHis, AviTag, and SUMOExpressionBacterialPromoterT7Available SinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-P_cymR-PCymRC
Plasmid#204071PurposeType 0 promoter partDepositorInsertcymR repressor and PCymRC cumic acid-inducible promoter
ExpressionBacterialMutationNoneAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCPP5099
Plasmid#128714PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopK1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5328
Plasmid#128721PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopU1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5306
Plasmid#128712PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopH1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5571
Plasmid#128713PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopI1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5539
Plasmid#128728PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopAF1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5618
Plasmid#128709PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopE1
ExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5617
Plasmid#128706PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopB1
ExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
araC-pBAD-gusA-pBAV1k
Plasmid#30502DepositorAvailable SinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pH6-spTorA-mCherry
Plasmid#169040PurposemCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.DepositorInsertH6-spTorA-mCherry
Tags6xHis and spTorA (signal peptide of E. coli TorA)ExpressionBacterialPromoterT7Available SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R1079A
Plasmid#83494PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)DepositorInsertLbu_C2c2_R1079A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR1079APromoterT7Available SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNRB150
Plasmid#36451DepositorAvailable SinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-RhoA Q63L
Plasmid#81046Purposemammalian expression of Zdk1 tagged RhoA Q63LDepositorAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
ATAD2
Plasmid#38916PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWT009d
Plasmid#107888PurposeR6 plasmid. Contains PBAD-EGFP and is part of part of the CAMERA system for rewritable multi-event analog recording in bacteriaDepositorInsertPBAD-EGFP
ExpressionBacterialAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRL692
Plasmid#70284PurposeTn5-based plasmid for transposition in Synechococcus sp. strain PCC 7942DepositorInsertTransposon Tn5-692
ExpressionBacterialAvailable SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-MBP-CfaN
Plasmid#126016PurposeBacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)DepositorInsertHis5-Sumo-MBP-CfaN
Tags5xHis-SUMOExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero11
Plasmid#187928PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero11DepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTST6
Plasmid#86603PurposeCustom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.DepositorInsertMTST6
ExpressionBacterialPromoterT7Available SinceSept. 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSPB845
Plasmid#128296Purposeasymmetric plasmid partitioning E. coli - regulatory plasmidDepositorInsertPLlacO1 - lp-cfp-parB, BBa_J23110 - lacI
ExpressionBacterialAvailable SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only