32,838 results

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pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer

Plasmid

#176588

Purpose

Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

CRISPR-associate IscB (Delaware Bay) locus

Expression

Bacterial

Mutation

Replaced spacer in CRISPR array with Fn spacer

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_hsHUR-Halo

Plasmid

#175303

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HUR (ELAVL1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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ShadowG-SUMO1

Plasmid

#175239

Purpose

Bacterial expression and purification, conjugatable SUMO for SUMOylation

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

SUMO1 (SUMO1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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polyPRM5

Plasmid

#175232

Purpose

Bacterial expression and purification, mixed with polySH3 to form condensates

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

ABL1 (ABL1 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLG169

Plasmid

#124367

Purpose

Xylose inducible system with the CRE site for expression in B. subtilis

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2 (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

Pxyl

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMCSG7(gfcC)

Plasmid

#176197

Purpose

gfcC protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcC

Tags

6xHis-TeV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_mmHUR-Halo

Plasmid

#175311

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HUR (Elavl1 Mouse)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-HBB-nLuc

Plasmid

#175432

Purpose

Used to generate IVT RNA for translation extracts

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

HBB-nLuc

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172504

Purpose

Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_BRD2-Halo

Plasmid

#175336

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

BRD2 (BRD2 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF RBP-FL P53

Plasmid

#174042

Purpose

Express Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)

Depositor

Stewart Loh

Article

Blanden et al Elife. 2020 Dec 2;9. pii: 61487. doi: 10

Insert

P53 gene (TP53 Human)

Tags

Thermoanaerobacter Tencongenesis ribose binding p…

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCK255

Plasmid

#171155

Purpose

Expression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

I-SceI_sacB

Expression

Bacterial

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII

Plasmid

#174424

Purpose

Expresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Tags

6xHis(Thrombin)EGFP(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pETx-SR6c3

Plasmid

#176211

Purpose

Bacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6c3

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR R4 ATCC LacZ GCTT

Plasmid

#173668

Purpose

Destination vector for Golden Gate Cloning assemblies, blue-white screening

Depositor

Eric Marois

Article

Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

Type

Empty backbone

Expression

Bacterial and Insect

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delCAT

Plasmid

#173942

Purpose

Bacterial expression of PARP1 lacking CAT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delCAT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 661-1014

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pnCS_SEPT7_SEPT9_i3-TEV-Strep

Plasmid

#174501

Purpose

bacterial co-expression of human SEPT7 and of human SEPT9_i3

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 Human)

Tags

TEV-Strep

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD_Ub11TCA

Plasmid

#174517

Purpose

Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Insert

Ubiquitin

Tags

6xHis

Expression

Bacterial

Mutation

Recoded with TCA replacing codon 11 of wt ubiquit…

Promoter

araBAD

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKV NS1

Plasmid

#145342

Purpose

Cell-free/mammalian expression vector for Zika virus NS1 protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS1

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pJH474_pTech_Para-supP

Plasmid

#163718

Purpose

Arabinose-inducible expression of supP

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

supP

Expression

Bacterial

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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MP6.6TAG_AraC_Para-dnaQ926-dam-seqA-emrR-ugi-CDA1.6TAG

Plasmid

#163719

Purpose

Arabinose-inducible expression of six mutagenesis genes, each encoding one amber codon after the initiator methionine

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TAG CDA1.1TAG

Expression

Bacterial

Mutation

dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TA…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH645

Plasmid

#172169

Purpose

Reporter plasmid for pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Pem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT

Expression

Bacterial

Mutation

Inserted amber codon immediately after initiator …

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINO_F2

Plasmid

#171926

Purpose

Bacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2

Depositor

Ray Owens

Article

Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

Insert

VHH_F2

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Available Since

Sept. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET28_6xHis-PARP1-ART

Plasmid

#174810

Purpose

Bacterial expression of isolated PARP1 ART subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-ART (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-BRCT

Plasmid

#174807

Purpose

Bacterial expression of isolated PARP1 BRCT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-BRCT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-WGR

Plasmid

#174808

Purpose

Bacterial expression of isolated PARP1 WGR domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-WGR (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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PdnaK GFP pZa

Plasmid

#170073

Purpose

GFP expression under the control of E. coli dnaK promoter

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PdnaK

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPGC-C

Plasmid

#174580

Purpose

pOPIN-GG expression vector compatible with C-terminal tagging, carbenicillin resistant

Depositor

Mark Banfield

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-HA2-GFP-HiBit

Plasmid

#171017

Purpose

For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-HA2-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pED14xS-1

Plasmid

#134800

Purpose

IPTG inducible expression of S-TAGA qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

S-TAGA qtRNA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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