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LVXN-Neo-NSD2

Plasmid

#86010

Purpose

express wild-type NSD2 in mammalian cells

Depositor

Darrin Stuart

Publication

Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

Insert

NSD2 (NSD2 Human)

Use

Lentiviral

Tags

Flag tag D Y K D D D D K

Expression

Bacterial

Mutation

K1002R (please see depositor comments below)

Promoter

CMV

Available since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFNF

Plasmid

#22687

Depositor

Robert Benezra

Publication

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Neo*

Use

Mouse Targeting

Expression

Bacterial and Mammalian

Mutation

FRT-pPGK-pEM7-Neo*-bpA-FRT

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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mRFP-human APPL1

Plasmid

#22202

Depositor

Pietro De Camilli

Publication

Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)

Tags

mRFP

Expression

Bacterial and Mammalian

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET12a-PAPI

Plasmid

#19961

Depositor

Jonathan Tait

Publication

Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

Insert

Annexin V (ANXA5 Human)

Expression

Bacterial

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-C3-hYAP2

Plasmid

#17844

Depositor

Marius Sudol

Publication

Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YAP (YAP1 Human)

Tags

EGFP

Expression

Bacterial and Mammalian

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSecUAG-Evol2

Plasmid

#163148

Purpose

Expresses machinery necessary for selenocysteine incorporation at UAG codons

Depositor

Dieter Söll

Publication

Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2

Inserts

allo-tRNA UTu2D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
Cysteine desulfurase

Expression

Bacterial

Mutation

Cysteine 32 switched to Selenocysteine (UAG), Cys…

Promoter

EM7, araBAD, native As SelD promoter, and native …

Available since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCT5-bac2.0

Plasmid

#119872

Purpose

Cumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

Depositor

Claudia Schmidt-dannert

Publication

Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

Inserts

CymR
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pveg promoter with CuO sequence and PxylR promoter

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a-AviTag-SnoopLigase

Plasmid

#105626

Purpose

Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation

Depositor

Mark Howarth

Publication

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

AviTag-SnoopLigase

Expression

Bacterial

Promoter

T7

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET28a-sfGFP 150TAG

Plasmid

#85493

Purpose

pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Publication

Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

Insert

superfolder GFP 150TAG

Expression

Bacterial

Mutation

150 TAG (amino acid 150 mutated to stop codon)

Promoter

T7

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pTriEx-RhoA-wt_mScarlet-i_SGFP2

Plasmid

#85071

Purpose

Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET/ELP-I-CAT

Plasmid

#71461

Purpose

ELP-intein purification system

Depositor

David Wood

Publication

Banki et al Nat Methods. 2005 Sep;2(9):659-61.

Type

Empty backbone

Tags

ELP ((VPGXG)110) and intein

Expression

Bacterial

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP E17TAG

Plasmid

#68295

Purpose

E17TAG GFP reporter

Depositor

Jesse Rinehart

Publication

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

E17TAG GFP

Tags

His

Expression

Bacterial

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDiGc

Plasmid

#59322

Purpose

Inducible expression of dsRed and constitutive expression of GFP in bacteria

Depositor

Sophie Helaine, David Holden

Publication

Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

Inserts

EGFP
dsRed

Expression

Bacterial

Promoter

Pbad and rpsM

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pRSETa_ mEos4b

Plasmid

#51073

Purpose

Photoconverible Fluorescent Protein_ Fixative Resistant (monomeric)

Depositor

Loren Looger

Publication

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

mEos4b

Expression

Bacterial

Promoter

T7

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGEX-2TK-14-3-3 eta GST

Plasmid

#13277

Depositor

Michael Yaffe

Publication

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 eta (YWHAH Human)

Tags

GST

Expression

Bacterial

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
spNW30

Plasmid

#173485

Purpose

Expresses spNW30 in Bl21

Depositor

Huan Bao

Publication

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW30

Tags

Histag

Expression

Bacterial

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pNeae2

Plasmid

#168300

Purpose

Bacterial surface display of polypeptides (nanobodies) in E. coli

Depositor

Luis Ángel Fernández

Publication

Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:

Insert

Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus

Tags

6xHis, E-tag, and myc-tag

Expression

Bacterial

Promoter

LacI-Plac

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDB302

Plasmid

#113673

Purpose

expresses Cth SUMO protease in Escherichia coli

Depositor

Christopher Bahl

Publication

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Cth SUMO protease

Tags

10-His

Expression

Bacterial

Mutation

deleted amino acids 1-934

Promoter

T7

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCT310

Plasmid

#111140

Purpose

Bacterial expression of 6His-dCas9-3XFlag

Depositor

Robert Tjian

Publication

Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

Insert

dCas9

Tags

3X Flag and 6X His

Expression

Bacterial

Mutation

D10A - H840A

Promoter

T7

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a-MSP1D1deltaH4H5

Plasmid

#71716

Purpose

Membrane scaffold protein (MSP), helix 4+ helix 5 deletion

Depositor

Gerhard Wagner

Publication

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Expression

Bacterial

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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