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pET14b – E. coli Exonuclease I

Plasmid

#104552

Purpose

bacterial expression of Exonuclease I

Depositor

Charles Bell

Article

Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

Insert

Exonuclease I (sbcB E.coli)

Use

Tags

6X-His tag followed by thrombin site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-HA-hM4D(Gi)

Plasmid

#45548

Purpose

Expresses hM4D (Gi) neuronal inhibitor

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

hM4D (Gi)

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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p6XHis_NLS-SaCas9

Plasmid

#101086

Purpose

Express SaCas9 in bacteria with a 6xHis tag for purification

Depositor

Rick Tarleton

Article

Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

Insert

CRISPR-associated protein Cas9/Csn1

Use

CRISPR

Tags

TrxA-6xHis-STag

Expression

Bacterial

Mutation

Promoter

T7lac

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-JNK1a1

Plasmid

#47574

Purpose

Bacterial expression plasmid for GST-3xFlag-tagged JNK1a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

JNK1a1 (MAPK8 Human)

Use

Tags

3xFlag and GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pH6-MBP-TEV-BirA

Plasmid

#179694

Purpose

Expresses His6 tagged MBP-BirA fusion protein

Depositor

Mark Arbing

Insert

BirA

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET-pAG-Hia5

Plasmid

#174373

Purpose

Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

Depositor

Aaron Straight

Article

Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

Insert

protein AG - hia5

Use

Tags

6xHis and Protein A/G

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pWUR790

Plasmid

#101723

Purpose

Synthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgo

Depositor

John van der Oost

Article

Swarts et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

PfAgo

Use

Tags

Strep(II)-tag

Expression

Bacterial

Mutation

Promoter

T7 Promotor

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pQE-60NA-msGFP2

Plasmid

#135301

Purpose

Encodes a photostable monomeric superfolder GFP

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

msGFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Duet1-His-TEV-mCherry-p62

Plasmid

#199780

Purpose

Used for the expression of mCherry labelled p62 (SMC Internal No. 391).

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Use

Tags

6x His, TEV, mCherry

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-GINKO2

Plasmid

#177116

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2

Depositor

Robert Campbell

Article

Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

Insert

GINKO2

Use

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pPSU20

Plasmid

#173829

Purpose

Expresses 20 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACCBPv3

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI-pheS

Plasmid

#111611

Purpose

Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Insert

pheS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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CAGGS-MCS-NLS-3XFLAG-V5

Plasmid

#196383

Purpose

Empty backbone vector to insert cDNA for strong expression in mammalian cells

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Type

Empty backbone

Use

Tags

3xFLAG and V5

Expression

Bacterial and Mammalian

Mutation

Promoter

CAGGs

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTKLP-tetA

Plasmid

#71325

Purpose

tetA landing pad template plasmid

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_MYRF

Plasmid

#64843

Purpose

A lentiviral vector for the expression of mouse MYRF under doxycyline control.

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

MYRF (Myrf Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pTKRED

Plasmid

#41062

Depositor

Thomas Kuhlman, Edward Cox

Article

Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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GST-TAX1BP1

Plasmid

#171423

Purpose

For the purifcation of TAX1BP1 protein

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGST2-GST-TEV-OPTN

Plasmid

#198428

Purpose

Used for the expression and purification of OPTN (WT) (SMC Internal No. 1587)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

optineurin (OPTN Human)

Use

Tags

GST and TEV

Expression

Bacterial

Mutation

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBlueKan+cysMPro

Plasmid

#187879

Purpose

Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.

Depositor

Gaylen Uhlich

Article

Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

CysM

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-IPP1-His

Plasmid

#118978

Purpose

Bacterial expression plasmid for Inorganic pyrophosphatase

Depositor

Sebastian Maerkl

Article

Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

Insert

inorganic diphosphatase IPP1 (IPP1 Budding Yeast)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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