32,218 results

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mTB3-Myc tag

Plasmid

#210205

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

Myc-tag

Expression

Bacterial

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMB2a

Plasmid

#190120

Purpose

Level 2 MoClo plasmid for A. baumannii

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCB11

Plasmid

#201111

Purpose

pSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.

Depositor

Mary Dunlop

Article

Lugagne et al Nat Commun. 2024 Mar 8;15(1):2148. doi:

Inserts

CcaR
tetA(C)
sfGFP

Expression

Bacterial

Mutation

C-to-T transition at pos. no. 1049 of tetA(C) lea…

Promoter

J23100 and PcpcG2-172

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSC301

Plasmid

#31851

Depositor

Yossef Av-Gay

Article

Cowley et al Gene. 2001 Feb 21;264(2):225-31.

Insert

GFP (including ATG start codon)

Expression

Bacterial

Promoter

SOD

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
ZP10 Snail

Plasmid

#16931

Depositor

Randall Moon

Insert

snail (snai1a Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pET-UbcH10-wt

Plasmid

#8503

Depositor

Joan Ruderman

Article

Bastians et al Mol Biol Cell 1999 Nov;10(11):3927-41.

Insert

UbcH10 (UBE2C Human)

Tags

His

Expression

Bacterial

Available Since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
pDM098

Plasmid

#216818

Purpose

Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mStayGold

Tags

FLAG-(SGGS)x2-XTEN16-(GGGGS)x3

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDUP1

Plasmid

#223516

Purpose

inactive cogfp under control of Ptet, cogfp under control of Ptac

Depositor

Yolanda Schaerli

Article

Mihajlovic et al eLife. 2025 Apr 2;13:RP97216. doi: 10.75

Inserts

CoGFP
CoGFP inactivated

Tags

His tag

Expression

Bacterial

Mutation

anti-dimerization mutations S129G, 22 C154S, D156…

Promoter

Ptac, Ptac and Ptet, and Ptet

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBad-bapaUGAc

Plasmid

#201673

Purpose

boronic acid- and peptide-assisted UDP-GlcNAc sensor

Depositor

Huiwang Ai

Article

Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acss

Insert

bapaUGAc

Tags

Hisx6

Expression

Bacterial

Promoter

araBAD

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_RAB5A_Ras

Plasmid

#109935

Purpose

Protein expression and purification of RAB5A_Ras

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RAB5A_Ras

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET-NtLAtC60αβC20

Plasmid

#160873

Purpose

Expression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcL, cpn60α, cpn60β, cpn20

Expression

Bacterial

Promoter

rbcL: pBAD, chaperones: T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMS1: pHelper(AvCAST)_AvPSP1

Plasmid

#168133

Purpose

Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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ELF4_pet28a

Plasmid

#131661

Purpose

Bacterial expression of ETS gene ELF4

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF4 (ELF4 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pH

Plasmid

#64178

Purpose

harbours marker gene HIS3

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-S18

Plasmid

#127827

Purpose

Production of recombinant human ribosomal protein S18

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS18 (RPS18 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b/BOA

Plasmid

#82041

Purpose

For E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)

Depositor

Angela Gronenborn

Article

Whitley et al FEBS J. 2013 May;280(9):2056-67. doi: 10

Insert

Burkholderia oklahomensis agglutinin

Tags

6x His

Expression

Bacterial

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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p_SNIPR_target_mut_36

Plasmid

#139466

Purpose

T7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 36

Depositor

Alexander Green

Article

Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

Insert

Target_mut36

Expression

Bacterial

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pJK_ToeRepG2_N02_switch

Plasmid

#132742

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 2 switch RNA with a GFP-ASV reporter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N02_switch

Expression

Bacterial

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1-sfGFP

Plasmid

#103983

Purpose

Plasmid encoding sfGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

sfGFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET30-2-PHGDH-C234S

Plasmid

#107724

Purpose

Expression of PHGDH C234S in bacteria

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

PHGDH (PHGDH Human)

Expression

Bacterial

Mutation

C234S

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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ZP17 Krox20

Plasmid

#16924

Depositor

Randall Moon

Insert

Krox20 (egr2b Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pAC-EsaR-EsaI

Plasmid

#47660

Purpose

esaI gene downstream of esaR in pAC-EsaR (CmR)

Depositor

Cynthia Collins

Article

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

esaI-term

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plac

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
DLG4_PDZ_3

Plasmid

#103953

Purpose

Protein expression and purification of PSD95 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

DLG4 (DLG4 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-1

Plasmid

#172767

Purpose

Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-1

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP6

Plasmid

#80662

Purpose

Encodes the synthetic coiled coil peptide SYNZIP6

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP6

Tags

6xHis

Expression

Bacterial

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP5

Plasmid

#80661

Purpose

Encodes the synthetic coiled coil peptide SYNZIP5

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP5

Tags

6xHis

Expression

Bacterial

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsNot1iso2(MCSI)_O

Plasmid

#147088

Purpose

Bacterial Expression of HsNot1iso2

Depositor

Elisa Izaurralde

Insert

HsNot1iso2 (CNOT1 Human)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET22-His-TEV-DOT1L-MM

Plasmid

#89611

Purpose

Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416

Depositor

Leemor Joshua-Tor

Article

Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

Insert

Dot1L VVEL293MM Mutant (DOT1L Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pH6HTC_mmHUR-Halo

Plasmid

#175311

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HUR (Elavl1 Mouse)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBb(B5)8k-sfGFP

Plasmid

#110140

Purpose

IPTG-inducible pTRC promoter expressing sfGFP on BBR1-B5 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

sfGFP

Expression

Bacterial

Available Since

July 9, 2018

Availability

Academic Institutions and Nonprofits only

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