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32,114 results

Showing: 6941 - 6960 of 32114 results

  1. pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1

    Plasmid
    #164614
    Purpose
    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.
    Depositor
    Jeffrey Chao
    Article
    Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.
    Insert
    stdMCP-stdHalo-RH1
    Use
    Lentiviral
    Tags
    HA tag and NLS
    Expression
    Bacterial and Mammalian
    Promoter
    UbiC
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164614-plasmid-map-sequence-id-325003

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  2. SHMT2

    Plasmid
    #25479
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SHMT2 (SHMT2 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    A269T
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25479-plasmid-map-sequence-id-168679

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  6. pUC19_CDC45_dTAG

    Plasmid
    #232733
    Purpose
    Generates CDC45 dTAG C ternimal knock-in cell lines as donor
    Depositor
    Junjie Chen
    Insert
    CDC45 (CDC45 Human)
    Expression
    Bacterial
    Mutation
    Synonymous mutation
    Available Since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    232733-plasmid-map-sequence-id-465446

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  7. pX459_MCM2_sgRNA

    Plasmid
    #232730
    Purpose
    Generates MCM2 dTAG C ternimal knock-in cell lines
    Depositor
    Junjie Chen
    Insert
    MCM2 (MCM2 Human)
    Expression
    Bacterial and Mammalian
    Mutation
    NO
    Available Since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232730-plasmid-map-sequence-id-465451

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  8. pX459_GINS4_gRNA

    Plasmid
    #232734
    Purpose
    Generates GINS4 dTAG C ternimal knock-in cell lines
    Depositor
    Junjie Chen
    Insert
    GINS4 (GINS4 Human)
    Expression
    Bacterial and Mammalian
    Mutation
    NO
    Available Since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232734-plasmid-map-sequence-id-465450

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  9. pX459_CDC45_sgRNA

    Plasmid
    #232732
    Purpose
    Generates CDC45 dTAG C ternimal knock-in cell lines
    Depositor
    Junjie Chen
    Insert
    CDC45 (CDC45 Human)
    Expression
    Bacterial and Mammalian
    Mutation
    NO
    Available Since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232732-plasmid-map-sequence-id-465449

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  10. pUC19_GINS4_dTAG

    Plasmid
    #232735
    Purpose
    Generates GINS4 dTAG C ternimal knock-in cell lines as donor
    Depositor
    Junjie Chen
    Insert
    GINS4 (GINS4 Human)
    Expression
    Bacterial
    Mutation
    Synonymous mutation
    Available Since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232735-plasmid-map-sequence-id-465448

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  11. pUC19_MCM2_dTAG

    Plasmid
    #232731
    Purpose
    Generates MCM2 dTAG C ternimal knock-in cell lines as donor
    Depositor
    Junjie Chen
    Insert
    MCM2 (MCM2 Human)
    Expression
    Bacterial
    Mutation
    Synonymous mutation
    Available Since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232731-plasmid-map-sequence-id-465447

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  13. pETSUMO2_Bacteroidetes-bGSDM-TEV

    Plasmid
    #222101
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Bacteroidetes-bGSDM-TEV ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    TEV protease site replaces residues E242–G248
    Promoter
    T7
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222101-plasmid-map-sequence-id-440273

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  14. pET16b-Rnl2(1-249)K227Q

    Plasmid
    #14072
    Depositor
    Thomas Tuschl
    Insert
    Rnl2(1-249)
    Tags
    His (10x)
    Expression
    Bacterial
    Mutation
    changed Lys227 to Glutamine Gly112 is actually C…
    Available Since
    Feb. 22, 2007
    Availability
    Academic Institutions and Nonprofits only

  15. pM9-BetaGal-8C

    Plasmid
    #172489
    Purpose
    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    M9-BetaGal-8C
    Tags
    6xHis and M9 nuclear localization sequence (from …
    Expression
    Bacterial
    Mutation
    BetaGal has two cysteine mutations (C77A, C1022S)…
    Promoter
    T7
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    172489-plasmid-map-sequence-id-356067

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  16. pJB02

    Plasmid
    #111959
    Purpose
    Expresses GST-GFP-DHFR; construct generated for pull-down and localization experiments
    Depositor
    Matthew Good
    Article
    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259
    Inserts
    mEGFP
    eDHFR
    Tags
    DHFR
    Expression
    Bacterial
    Mutation
    eDHFR;residues 2-159 and enhanced GFP with monome…
    Promoter
    T7
    Available Since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111959-plasmid-map-sequence-id-219913

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Showing: 6941 - 6960 of 32114 results