30,327 results
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pET30b-5M SrtA with TEV site
Plasmid#86962PurposeExpresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tagDepositorInsertS.aureus SrtA 5M
UseTags6x Histidine tag and TEV siteExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
6975 pPROEX-HTb-SIS1
Plasmid#1233DepositorInsertsis1 (SIS1 Budding Yeast)
UseTagshis tag (tev cleavable)ExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
H6-auxilin
Plasmid#62571Purpose6xHis-tagged full-length bovine brain auxilin (auxilin 1) in pQE30 for protein expression in bacteriaDepositorInsertAuxilin
UseTags6XHisExpressionBacterialMutation*see comment belowPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
ARF1 (ARF1A-c001)
Plasmid#110258PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertARF1 (ARF1 Human)
UseTagsStrepII-TEVExpressionBacterialMutationPromoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_41
Plasmid#59964PurposeHigh full length T7 RNAP* expressionDepositorInsertT7* RNAP
UseTagsExpressionBacterialMutationN/APromoterPJ23105Available sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-mBoNT/F
Plasmid#168015PurposeExpresses MBP-BoNT/F-LC(1-428)-6xHis in bacterial cellsDepositorInsertMBP-BoNT/F-LC(1-428)-6xHis
UseTagsHisx6 and maltose binding proteinExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-2_LC3A
Plasmid#73946PurposeFor recombinant expression of human LC3A/MAP1LC3A in E. coliDepositorInsertLC3A (MAP1LC3A Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-pck_NT
Plasmid#74069PurposepAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecRDepositorInsertsgRNA pck (NT)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-LIN28
Plasmid#16350DepositorInsertHomo sapiens lin-28 homolog (C. elegans) (LIN28A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pRSAN-QUE37C
Plasmid#129312PurposeATP measurementDepositorInsertQUEEN-37C
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3990 pET ubcH8
Plasmid#8650DepositorInsertUbcH8 (UBE2L6 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLP-pTac-Tn5-chlorR
Plasmid#199994PurposeLanding Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.DepositorInsertLanding Pads
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPath-pTac-integrase
Plasmid#199999PurposeEntry vector for biosynthetic pathway. pTac-phiC31 integrase. This material contains 2 plasmids. For more information look under the "Depositor Comments" section below.DepositorInsertPathways
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQlinkH-PTPIP51(36-235)
Plasmid#170533PurposeExpresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsHis tagExpressionBacterialMutationPromoterT5 promoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTJV1ScKm-rpoB
Plasmid#166980PurposeGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistanceDepositorInsertNeoR/KanR
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-RNAPII-CTD wt #14-HA
Plasmid#160688PurposeExpression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.DepositorInsertRNA Polymerase II CTD
UseTagsExpressionBacterialMutationC terminal domain onlyPromoterAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-FOXP2_P6
Plasmid#122649PurposeExpresses truncated variant (aa271-aa500) of human FOXP2 in bacterial cellsDepositorInsertHomo sapiens forkhead box P2 (FOXP2 Human)
UseTagsHis-tag and Strep-tag IIExpressionBacterialMutationPromoterT7Available sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only
