32,218 results

Showing: 10441 - 10470 of 32218 results

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pflaG

Plasmid

#45477

Depositor

Michele Swanson

Article

Hammer et al Mol Microbiol. 1999 Aug;33(4):721-31.

Insert

flaA-GFP

Expression

Bacterial

Promoter

flaA

Available Since

June 4, 2013

Availability

Academic Institutions and Nonprofits only
TOPO CFP

Plasmid

#68489

Purpose

TOPO construct expressing CFP for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cyan Fluorescent Protein

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBT3024

Plasmid

#122542

Purpose

Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_mt::CemOrange2

Tags

MLSLRQSIRFFKPATRTLCSSRTLL

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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NMT1

Plasmid

#25274

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

NMT1:T108-Q496 (NMT1 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 20, 2010

Availability

Industry, Academic Institutions, and Nonprofits
LB124 (Bcl-2 from -1287 to -3934)

Plasmid

#15240

Depositor

Linda Boxer

Article

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -3934 with P1

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
BPV00554 pET21GG2-His(6)-BECN(248-450)

Plasmid

#136685

Purpose

Expresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a N-terminal hexa-Histidine tag

Depositor

Colin Garvie

Article

Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

Insert

Beclin-1 ECD/BARA (BECN1 Human)

Tags

Hexa-Histadine

Expression

Bacterial

Mutation

DNA optimized for E. coli expression

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET23b-proMMP-7

Plasmid

#29524

Depositor

William Parks

Article

Ra et al J Biol Chem. 2009 Oct 9. 284(41):27924-3

Insert

proMMP-7 (MMP7 Human)

Expression

Bacterial

Available Since

April 29, 2011

Availability

Academic Institutions and Nonprofits only
Fusion of Dengue2 NS2B-NS3 proteins

Plasmid

#204792

Purpose

Bacterial expression of a fusion of Dengue2 NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue2 NS2B-NS3 fusion

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHH0103_THRA_Hormone-recep

Plasmid

#109810

Purpose

Protein expression and purification of THRA_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

THRA_Hormone-recep (THRA Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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p2207 pGEM p53

Plasmid

#10853

Depositor

Peter Howley

Article

Werness et al Science. 1990 Apr 6. 248(4951):76-9.

Insert

p53 (TP53 Human)

Expression

Bacterial

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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HK3

Plasmid

#25303

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

HK3:R480-R922 (HK3 Human)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids R480-R922

Available Since

Aug. 16, 2010

Availability

Industry, Academic Institutions, and Nonprofits
hHpr1-GST

Plasmid

#11200

Depositor

Robin Reed

Article

Masuda et al Genes Dev. 2005 Jul 1. 19(13):1512-7.

Insert

Hpr1 (THOC1 Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only
pIntegrase_10

Plasmid

#60581

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_10

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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pTetQCas-BsaI

Plasmid

#170629

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)

Expression

Bacterial

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pEB1-mTurquoise2

Plasmid

#103974

Purpose

Plasmid encoding mTurquoise2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mTurquoise2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET30b-7D-SrtA

Plasmid

#105600

Purpose

expresses 7D sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7D mutant

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTetR193-Wasabi

Plasmid

#86105

Purpose

mWasabi reporter gene gated by temperature-sensitive TetR I193N

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TetRts
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

I193N

Promoter

ptetA and ptetR

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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POLArIS_NbVim-cp10GFP

Plasmid

#171020

Purpose

Expresses NbVim-cp10GFP#2 in mammalian cells

Depositor

Sumio Terada

Article

Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

Insert

anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)

Tags

cp10GFP

Expression

Bacterial and Mammalian

Promoter

CMV promoter

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_BCL2L1_BH4+Bcl-2

Plasmid

#109993

Purpose

Protein expression and purification of BCL2L1_BH4+Bcl-2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

BCL2L1_BH4+Bcl-2 (BCL2L1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-T7-Top-T

Plasmid

#87309

Purpose

Expresses the "Top" transcript of the Split-Broccoli system under the control of the T7 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Top" transcript

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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BB3rN_14*

Plasmid

#98550

Purpose

empty BB3 for insertion and overexpression of 1 gene in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.944

Plasmid

#108523

Purpose

PCin-YFP Reporter. OHC14 sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PCin-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBT3007

Plasmid

#122538

Purpose

Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a early endosome localized gene (rab-5) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemOrange2::rab-5 (rab-5 Nematode, Synthetic)

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEB2-TagRFP

Plasmid

#103997

Purpose

Plasmid encoding TagRFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

TagRFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, K42R, K67R, …

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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6974 pPROEX-HTb-SSB1

Plasmid

#1232

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

ssb1 (SSB1 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available Since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only
SEPTOPO

Plasmid

#64944

Purpose

source of SEP (Super Ecliptic pHluorin) sequence

Depositor

Jeremy Henley

Article

Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

Insert

SEP

Expression

Bacterial

Promoter

lac

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only
pQE60 R196M wgMDH

Plasmid

#204273

Purpose

Bacterial expression of R196M wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R196M

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 D193E wgMDH

Plasmid

#204269

Purpose

Bacterial expression of D193E wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

D193E

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET21b-LaG9(S151C)

Plasmid

#172490

Purpose

Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

Depositor

Siegfried Musser

Article

Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

Insert

LaG9(S151C)

Tags

6xHis, PPX cleavage site, and pelB leader sequenc…

Expression

Bacterial

Mutation

S151C

Promoter

T7

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pBbA13c-RFP

Plasmid

#126098

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23102

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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