32,218 results
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Plasmid#21025DepositorAvailable SinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only
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pET28_6xHis-PARP1-L713F
Plasmid#174794PurposeBacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-C125G
Plasmid#174792PurposeBacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSP2283
Plasmid#70702PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialPromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET3a-DJ1
Plasmid#51489PurposeInducible expression of human DJ1 in E.coliDepositorAvailable SinceMarch 31, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET15b/GgVcl 884-1066-ECFP
Plasmid#46182DepositorInsertVcl 884-1066 (VCL Chicken)
TagsECFP and HisExpressionBacterialMutationaa 884-1066 (tail domain)PromoterT7Available SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWH-DI
Plasmid#47385PurposeExpresses dxs and idi under PxylA promoter (inducible by xylose) in bacteriaDepositorInsertsdxs
idi
ExpressionBacterialPromoterPxylAAvailable SinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Med25-VBD-6His
Plasmid#64773PurposeBacterial expression of His-tagged Med25-VBDDepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Endosomal Escape Control
Plasmid#186552PurposeNegative Control confirming the need for a CPP peptide in mediating internalizationDepositorInsert6H-ETA-GFP
Tags6xHis and EGFPExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization and Endosomal Escape
Plasmid#186551PurposeInternalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cellsDepositorInsert10R-8His-ETA-EGFP
Tags8xHis and EGFPExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX Tec-SH2
Plasmid#46522DepositorInsertTec (TEC Human)
TagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 236-351PromoterTacAvailable SinceOct. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-human MIZ1 wild type
Plasmid#74161Purposebacterial expression of MIZ1DepositorAvailable SinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_C1
Plasmid#171924PurposeBacterial expression of nanobody VHH_C1, which binds to receptor binding domain of the spike protein of SARS-CoV-2DepositorInsertVHH_C1
TagsHis6ExpressionBacterialAvailable SinceSept. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSF-G1(6CNW)RS
Plasmid#222873PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pThuS
Plasmid#171674PurposeThuS expression in E. coliDepositorInsertGlycosyltransferase ThuS
TagsHis-tagExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-dHer1cys
Plasmid#73218PurposeBacterial expression vector for Z1907 affibody protein (A) with N-terminal His10, GST and HRV3C cleavage site (cysteine tag)DepositorInsert10XHis-GST-HRV-dHer1cys
Tags10XHis-GST-HRV and The dimer of the Her1 Affibody…ExpressionBacterialPromoterT7Available SinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPS0314
Plasmid#115507PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celBDepositorInsertcelB pOGG050, T-pharma pOGG003 assembled in pOGG026
ExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterConstitutive promoter pNeo pOGG001Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS hTSP-3
Plasmid#12995DepositorInsertTSP-3 (THBS3 Human)
ExpressionBacterialAvailable SinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGT2
Plasmid#13056DepositorTypeEmpty backboneExpressionBacterialAvailable SinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
GST-Cav FL (1-178)
Plasmid#22445DepositorInsertCaveolin1(1-178)
TagsGSTExpressionBacterialAvailable SinceDec. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rha
Plasmid#129390PurposeDerived from mini-CTX1. An integrase vector that can integrate the rhamnose-inducible promoter system into the chromosome of a host bacterium.DepositorInsertRhamnose-inducible promoter
ExpressionBacterialMutationN/APromoterPrhaBADAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pANTO5
Plasmid#131106PurposeTo generate an integrative plasmid carrying the TetR/Pip-OFF system, but not the integraseDepositorInserttetR gene by pFRA61 (Boldrin et al 2010)
ExpressionBacterialAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
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HC89 pComb3xss anti-Homer1 nanobody
Plasmid#134719PurposeBacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-Homer1 (Mouse) recombinant llama nanobody HC89 (Homer1 L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (64-502, construct 13, MG-26-82)
Plasmid#110746PurposeBacterial expression of human USP30 (construct 13)DepositorInsertUSP30 (USP30 Human)
TagsN-His6-3C-GST-3CExpressionBacterialMutationF348D, M350S, I353E + insertion deletion: 64-178;…PromoterT7Available SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta-mmr
Plasmid#169241PurposeCas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutationsDepositorInsertscas9
beta
mutL-E36K
UseCRISPR and Synthetic BiologyExpressionBacterialMutationChanged glutamic acid 36 to lysineAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHL 1903
Plasmid#53020PurposeAmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1DepositorInsertGFP
UseSynthetic BiologyTagsAAV, Asp terminator, and fimSExpressionBacterialPromoterfimSAvailable SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGMCKq1-10M1-sspBmyc
Plasmid#27069DepositorInserttetR-PmyctetO-sspB
ExpressionBacterialAvailable SinceJune 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template2-3XMyc-Spacer
Plasmid#176209PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template2-3XMyc-Spacer
TagsMyc tagExpressionBacterialPromoterT7Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
RHOB
Plasmid#38801PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only