30,808 results

1. hsp70: mKate2-DN Kif5a

   Plasmid

   #105972

   Purpose

   heat shock inducible transgenesis

   Depositor

   Caren Norden

   Article

   Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

   Insert

   Kif5a (kif5aa Zebrafish)
   

   Tags

   mKate2

   Expression

   Bacterial

   Mutation

   truncation of aa416-1033

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PNMT

   Plasmid

   #64078

   Purpose

   Expression of human Phenylethanolamine N-methyltransferase

   Depositor

   Vern Schramm

   Article

   Stratton et al ACS Chem Biol. 2017 Feb 17;12(2):342-346

   Insert

   Phenylethanolamine N-methyltransferase (PNMT Synthetic, Human)
   

   Tags

   Histidine tag

   Expression

   Bacterial

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pOPS0377

   Plasmid

   #115509

   Purpose

   Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFP

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Constitutive promoter pNeo pOGG001

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSET FLII12Pglu-700uDelta6

   Plasmid

   #13568

   Depositor

   Wolf Frommer

   Article

   Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

   Insert

   FLIPglu F16A Y12S-ECFP-D13R, 6aa linker
   

   Tags

   Citrine

   Expression

   Bacterial

   Mutation

   F16A, ECFP insert in mglB

   Available Since

   Feb. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTO_CHMP2B_LAP

   Plasmid

   #101848

   Purpose

   Tet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

   Depositor

   Daniel Gerlich

   Article

   Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

   Insert

   CHMP2B (CHMP2B Human)
   

   Use

   Flp-frt recombination, tet-inducible expression

   Tags

   Localization and Affinity Purification (LAP) tag

   Expression

   Bacterial and Mammalian

   Mutation

   silent mutation in Thr 104; A to T

   Promoter

   CMV

   Available Since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. EC10161

   Plasmid

   #154060

   Purpose

   Level 0.5 Golden Gate backbone, containing loxP sites.

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Bacterial

   Promoter

   N/A

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST17-UbE2J2

   Plasmid

   #15794

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2J2 (UBE2J2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

8. ARH1-c003

   Plasmid

   #173093

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   ADPRH (ADPRH Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   E2-L357, codon optimized

   Available Since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)

   Plasmid

   #128165

   Purpose

   UV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).

   Depositor

   Marcus Noyes

   Article

   Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

   Insert

   10 amino acid glycine/serine linker + 5alanines
   

   Use

   Synthetic Biology

   Tags

   FLAG and zif268

   Expression

   Bacterial

   Promoter

   UV5

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJDC60

    Plasmid

    #157780

    Purpose

    tdTomato is expressed under the mycobacterial L5 promoter for in vivo and in vitro detection.

    Depositor

    Jeffrey Cirillo

    Article

    Kong et al Methods Mol Biol. 2018;1790:75-85. doi:

    Insert

    tdTomato
    

    Expression

    Bacterial

    Promoter

    L5

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. BB3nK_ext_AD

    Plasmid

    #108687

    Purpose

    Plasmid for assembly of donor DNA templates consisting of three expression units

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PclpB riboswitch GFP pZa

    Plasmid

    #170078

    Purpose

    GFP expression under the control of E. coli clpB promoter engineered with riboregulators

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PclpB

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. HS-H2B-RFP

    Plasmid

    #105953

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    H2B
    

    Tags

    RFP

    Expression

    Bacterial

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. His10-CD3z-SNAPf-ybbR (XSB507)

    Plasmid

    #78508

    Purpose

    Construct for expressing CD3z-SNAPf-ybbR in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    CD3z (Cytoplasmic domain) (CD247 Human)
    

    Tags

    His10 and SNAPf-ybbR

    Expression

    Bacterial

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCD565

    Plasmid

    #153027

    Purpose

    dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA

    Depositor

    Jesse Zalatan

    Article

    Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

    Insert

    dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC18T-mini-Tn7T-Zeo-eyfp

    Plasmid

    #65039

    Purpose

    ZeoR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    eyfp
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-GGG-ELP(120nm)-Cys

    Plasmid

    #91572

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    GGG-ELP(120nm)-Cys
    

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCK301

    Plasmid

    #87767

    Purpose

    E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

    Insert

    PrhaBAD-sfGFP
    

    Use

    Synthetic Biology

    Tags

    6xHis Tag

    Expression

    Bacterial

    Promoter

    PrhaBAD rhamnose-inducible promoter from E. coli

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. EID1-pGex-2TK

    Plasmid

    #22689

    Depositor

    William Kaelin

    Article

    Miyake et al Mol Cell Biol. 2000 Dec . 20(23):8889-90

    Insert

    E1A-like inhibitor of differentiation 1 (EID1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    wt cDNA

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX6P-1-SF2

    Plasmid

    #99020

    Purpose

    Bacterial expression of SF2

    Depositor

    Honglin Chen

    Article

    Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

    Insert

    SF2 (SRSF1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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