30,327 results
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Plasmid#127413PurposeExpresses DpnII restriction endonuclease in bacteriaDepositorInsertDpnII
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGTvL1-SGC
Plasmid#39188PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsGST and TEV cleavage siteExpressionBacterialMutationPromoterTac (lactose/IPTG inducible)Available sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHMattR3-attR4-GFP
Plasmid#196247PurposepHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP geneDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCORE-UK
Plasmid#72238PurposeContains the CORE cassette with convergent KlURA3 and KanMX4 markersDepositorInsertsKlURA3
kanMX4
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET co-transformation cloning vector (13S-A)
Plasmid#48323DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
B4-1
Plasmid#111475PurposeAla-tRNA synthetaseDepositorInsertAla-tRNA synthetase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_attP
Plasmid#110095PurposeVector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.DepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET19b+H2B
Plasmid#157809PurposeExpression of human histone H2BDepositorInsertH2B
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PP7v3
Plasmid#107230PurposeExpresses 24x optimized PP7 stem loops (version 3) in bacterial cellsDepositorInsert24xPP7v3
UseTags3x FLAG-tag and P2A siteExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPEPX-Plac
Plasmid#122632PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTC-28S15-0X
Plasmid#20316DepositorInsertPsmyc-tetR#28
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
Dead Streptavidin-SpyTag
Plasmid#59548PurposepET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramersDepositorInsertDead Streptavidin-SpyTag
UseTagsSpyTagExpressionBacterialMutationThe construct lacks final K of SpyTagPromoterT7Available sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCVL.Active/Repressed TLR (Sce)
Plasmid#45575PurposeCodes for the Active/Repressed TLR with the target site for I-Sce I nucleaseDepositorInsertseGFP with I-Sce I TS
+3 mCherry
UseLentiviralTagsT2A dislinker and iRFPExpressionBacterial and MammalianMutationembedded I-Sce I TS from 163-185PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delCAT
Plasmid#173942PurposeBacterial expression of PARP1 lacking CAT domainDepositorInsertPARP1delCAT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 661-1014PromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPEPY
Plasmid#122633PurposeBglFusion compatible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIV300
Plasmid#204968PurposeAMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance markerDepositorInsertsMad7
hph (hygromycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP12089
Plasmid#222357PurposeTn7 integrative vector with PabstBR promoter expression cassette and hygR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSPdH5
Plasmid#199771PurposeExpresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)DepositorInsertcMSPdH5
UseTagsHis6-TagExpressionBacterialMutationexternal His-Tags flanking the C and N terminiPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV313
Plasmid#204956PurposeAMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. nigerDepositorInsertsMad7
hph (hygromycin resistance marker)
sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus fumigatus U3 promoter, Aspergillus ni…Available sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-Cdc42-Q61L
Plasmid#12971DepositorInsertCdc42 constitutively active (CDC42 Human)
UseTagsGSTExpressionBacterialMutationQ61L Constitutively activePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only