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pMO746

Plasmid

#117480

Purpose

upp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanR

Depositor

Judy Wall

Article

Parks et al Science. 2013 Mar 15;339(6125):1332-5. d

Insert

uracil phosphoribosyltransferase

Expression

Bacterial

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pKB01_tagRFP

Plasmid

#69103

Purpose

Expresses tagRFP (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Article

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

tagRFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBD-0001

Plasmid

#12373

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP (Vcp Mouse)

Tags

His6

Expression

Bacterial

Available Since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only

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pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

Plasmid

#90473

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Insert

TagGFP2-TEV-GGG-ELP(120nm)-Cys

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCRII p15

Plasmid

#16454

Depositor

Bert Vogelstein

Article

Jen et al Cancer Res. 1994 Dec 15. 54(24):6353-8.

Insert

p15 (CDKN2B Human)

Expression

Bacterial

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-RBP-LOV2

Plasmid

#185792

Purpose

Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

Depositor

Lizhong He

Article

Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

Insert

RBP-LOV2

Tags

10xHis, TEV protease cleavage site after His Tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCK301

Plasmid

#87767

Purpose

E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI

Depositor

John Heap

Article

Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

Insert

PrhaBAD-sfGFP

Use

Synthetic Biology

Tags

6xHis Tag

Expression

Bacterial

Promoter

PrhaBAD rhamnose-inducible promoter from E. coli

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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ELK1_pet28a

Plasmid

#131647

Purpose

Bacterial expression of ETS gene ELK1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELK1 (ELK1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-Selo-FI-prk

Plasmid

#162698

Purpose

Expresses S. elongatus Form IB rubisco and S. elongatus Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

S. elongatus Form IB rubisco and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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WT A1-LCD +NLS

Plasmid

#178658

Purpose

Bacterial expression of N-terminally 6His tagged WT A1-LCD (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

WT A1-LCD +NLS (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCascade_SPS1(PmcCAST)

Plasmid

#224926

Purpose

Expression and purification of type I-B2 Cascade in CRISPR-associated transposon of Romsetta

Depositor

Leifu Chang

Article

Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

Inserts

Cas5, Cas6, Cas7, Cas8, Cas11
CRISPR assay for crRNA expression

Use

CRISPR

Expression

Bacterial

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET_StrepII_TEV_LIC_TniQ(PmcCAST)

Plasmid

#224927

Purpose

TniQ expression plasmid for the co-purification of TnsC-TniQ complex

Depositor

Leifu Chang

Article

Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

Insert

TniQ

Use

CRISPR

Expression

Bacterial

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80RFP-pXD68Kan2-RFP

Plasmid

#225329

Purpose

RSF1010-based broad host-range plasmid engineered from pXD68Kan2 encoding RFP, kanamycin resistance

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

RFP

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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QUAS-5-T7-TetO-GFP_ColE1

Plasmid

#171660

Purpose

QUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

QUAS-5-T7-TetO-GFP ssra-T7 Stop

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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T7-LacO-QF-T7-TetO-TetR_p15A

Plasmid

#171661

Purpose

Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. There is no T7 terminator sequence between the QF- and TetR-containing circuits. Contains a p15A origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

QF-T7-TetO-TetR

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_RBFOX2-Halo

Plasmid

#175304

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

RBFOX2 (RBFOX2 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-ZFD_L

Plasmid

#198276

Purpose

For loading L half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Left-G1397-N_DddA); also contains PVC regulatory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-ZFD_L

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-2

Plasmid

#172768

Purpose

Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJMP8045

Plasmid

#220900

Purpose

Tn7 transposase (tnsABCD) expression plasmid for Rhodobacter sphaeroides

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

tnsABCD

Expression

Bacterial

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pTAT2.1

Plasmid

#35620

Depositor

Steven Dowdy

Article

Wadia et al Nat Med. 2004 Mar;10(3):310-5. Epub 2004

Type

Empty backbone

Tags

6xHIS and TAT

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only