31,879 results
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Plasmid#239750Purposeguide for aeBlueDepositorInsertguide targeting aeBlue
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minTK
Plasmid#211516PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneExpressionBacterialPromoterSV40 and minTKAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1 ElonginB, ElonginC
Plasmid#230999PurposeDual expression vector containing mouse ElonginB and ElonginC for E.coli protein purificationDepositorAvailable SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDusk
Plasmid#43795PurposeEmpty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-His6MBP-A3A-Gly6His6
Plasmid#187822PurposeExpression human APOBEC3A in E.coli.DepositorInsertAPOBEC3A (APOBEC3A Human)
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationnonePromoterT7 promoter (on the backbone)Available SinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-SpCas9
Plasmid#117051PurposeIn vitro expression of S. pyogenes Cas9 from the T7 promoterDepositorInsertS. pyogenes Cas9
ExpressionBacterialPromoterT7Available SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-csgA
Plasmid#89581PurposeExpress csgA curli protein with GS linker and HistaqDepositorAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-sfGFP*-XcP4H-MH4R
Plasmid#178043PurposeExpress green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTPDepositorInsertssuperfolder green fluorescent protein with TAG at 151 position
XcP4H with W179F mutation
dihydropterine reductase from human
pterin-4a-carbinolamine dehydratase from human
TagsHis tagExpressionBacterialAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVoPo-GFP
Plasmid#187840PurposeConstitutive GFP-expression vector for Fusobacterium nucleatumDepositorInsertsfGFP
ExpressionBacterialAvailable SinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.5t
Plasmid#210257PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertseBFP2
superfolder GFP
TagsssraExpressionBacterialPromoteripaH7.8p and rpsMAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-pBAD
Plasmid#54519PurposeLocalization: Protein Expression Vector , Excitation: 485, Emission: 507DepositorTypeEmpty backboneTags6xHis and SfGFPExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPEPX-P3-sgRNAluc
Plasmid#85590PurposeIntegrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template forDepositorInsertsgRNA targeting firefly luciferase encoding gene
UseCRISPRExpressionBacterialPromoterP3Available SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJJS006
Plasmid#188329PurposePCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::mNeonGreen::mIAA7
ExpressionBacterialAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbS2k-RFP
Plasmid#35330DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterTetAvailable SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJC175e
Plasmid#79219PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPPSP gIII, luxAB
ExpressionBacterialPromoterPPSPAvailable SinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGPΩTp
Plasmid#130660PurposeInsertional mutagenesis of GP transposon into chromosome of B. cenocepacia. Trimethoprim selection for plasmid.DepositorInsertΩ trimethoprim cassette
ExpressionBacterialMutationAddition of Ω sites next to trimethoprim cassette…Promoterdhfr promoterAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
P74-26 Tail Tube Protein (gp93) in pSMT3
Plasmid#191261PurposeExpresses P74-26 gp93 with a His-SUMO tag in bacterial cellsDepositorInsertP74-26 gp93
TagsHis-SUMOExpressionBacterialPromoterT7Available SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDG02583
Plasmid#104129PurposeOptimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)DepositorInsertNEDD8-specific protease 1 (NEDP1)
Tags14x Histidine tag, Maltose binding protein, and S…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialPromoterT7Available SinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
VN1852 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP4_Ddcm_WeaKRBS_TIR51_KanR
Plasmid#235118PurposeQuantitative bacterial two-hybrid (qB2H). Expresses ASf1B and IP4.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterLtetO and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only