30,735 results

1. pMBP-MS2

   Plasmid

   #65104

   Purpose

   can be used to isolate RNA binding complexes

   Depositor

   Josep Vilardell

   Article

   Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

   Insert

   MS2
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   double mutation (V75Q and A81G) that prevents oli…

   Promoter

   tac

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUS_anion

   Plasmid

   #98656

   Purpose

   Expresses untagged FUS AA 1-163

   Depositor

   Nicolas Fawzi

   Article

   Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

   Insert

   FUS (FUS Human)
   

   Expression

   Bacterial

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-MH6-uvsY

   Plasmid

   #163914

   Purpose

   Expresses uvsY for bacterial expression and affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   uvsY (uvsY Enterobacteria phage T4)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-uvsX-H6

   Plasmid

   #163913

   Purpose

   Expresses uvsX for bacterial expression and affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   uvsX
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK19pA-MN

   Plasmid

   #86973

   Purpose

   ChIC; genomic mapping of chromatin proteins

   Depositor

   Ulrich Laemmli

   Article

   Schmid et al Mol Cell. 2004 Oct 8;16(1):147-57.

   Insert

   protein_A fused to Staphylococcus aureus microccocal nuclease (MNase)
   

   Expression

   Bacterial

   Available Since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA24N-p50-ligase

   Plasmid

   #87742

   Purpose

   IPTG-inducible expression of p50-ligase fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Tags

   6xHis and human p50/NF-kB (amino acids 40-366)

   Expression

   Bacterial

   Promoter

   T5-lac

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDae029 (eMutaT7[TadA-8e])

   Plasmid

   #187620

   Purpose

   Expresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coli

   Depositor

   Seokhee Kim

   Article

   Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

   Insert

   TadA-8e-T7RNAP
   

   Expression

   Bacterial

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDisCoTune

   Plasmid

   #173713

   Purpose

   Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

   Depositor

   Morten Norholm

   Article

   Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2

   Inserts

   ERV1 (ERV1 Budding Yeast)
   PDI
   

   Expression

   Bacterial

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPomyces-2BD

   Plasmid

   #234428

   Purpose

   Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template

   Depositor

   Min-Kyu Oh

   Article

   Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

   Insert

   Modified Cas9 with polyaspartate linking
   

   Expression

   Bacterial

   Mutation

   Linked polyaspartate using glycine-serine linker

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLumi-LgBiT

    Plasmid

    #188319

    Purpose

    Expression of LgBiT via sigma 70 promoter in E. coli

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    LgBiT
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Bacterial

    Promoter

    sigma 70 promoter

    Available Since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pyEvolvR (enCas9-PolI5M)

    Plasmid

    #158073

    Purpose

    Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber

    Article

    Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

    Insert

    enCas9-PolI5M
    

    Expression

    Bacterial and Yeast

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDx_mScarlet-I3

    Plasmid

    #189757

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I3
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTP1122

    Plasmid

    #104159

    Purpose

    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
    

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAUC40

    Plasmid

    #71298

    Purpose

    Suicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101

    Depositor

    Susanne von Bodman

    Article

    Carlier et al Mol Microbiol. 2009 Nov;74(4):903-13. do

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET His6 Thioredoxin TEV co-transformation cloning vector (13K-T)

    Plasmid

    #48322

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and Thioredoxin

    Expression

    Bacterial

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET Flag TEV LIC cloning vector (2L-T)

    Plasmid

    #29715

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    FLAG-TEV

    Expression

    Bacterial

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE30-His-CKM

    Plasmid

    #124134

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Creatine kinase (CKM Chicken)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVSV eGFP dG

    Plasmid

    #31842

    Depositor

    Connie Cepko

    Article

    Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    VSV genome
    

    Expression

    Bacterial

    Available Since

    Oct. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2+ EB3-mKate2

    Plasmid

    #105940

    Purpose

    in vitro transcription

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    EB3 (MAPRE3 Human)
    

    Tags

    mKate2

    Expression

    Bacterial

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. His6-pAG-RTase

    Plasmid

    #214932

    Purpose

    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

    Depositor

    Chuan He

    Article

    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

    Insert

    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

    Promoter

    T7 promoter

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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