33,902 results

Showing: 701 - 720 of 33902 results

Results per page:

Sort results by:

pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

Plasmid

#199390

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

Depositor

Rebecca Voorhees

Publication

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Tags

14xHis-Avi-SUMOEu

Expression

Bacterial

Promoter

T5-LacO

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDuEx2-NIR-GECO1

Plasmid

#113680

Purpose

Bacterial and mammalian expression plasmid for near-infrared fluorescent Ca2+ indicator NIR-GECO1

Depositor

Robert Campbell

Publication

Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NIR-GECO1

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pDNA2.0 6H-TEV-KRAS

Plasmid

#111849

Purpose

Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Publication

Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

Insert

KRAS (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Promoter

T5

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pSAM_AraC

Plasmid

#91569

Purpose

Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

Depositor

Harry Mobley

Publication

Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

Insert

AraC/PBAD

Expression

Bacterial

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

Enlarge
pJYS2_crtYf

Plasmid

#85544

Purpose

Constitutive transcription of crRNA of crtYf, Spectinomycin resistance

Depositor

Sheng Yang

Publication

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Insert

crRNA of crtYf

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Expression

Bacterial

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pET21b-Caspase-3

Plasmid

#90087

Purpose

Expresses wild type caspase 3 in bacterial cells

Depositor

Clay Clark

Publication

Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

Wild type

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pET-scFv-T

Plasmid

#67843

Purpose

pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

Depositor

Joanna Bereta

Publication

Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24

Insert

antibody 1D8 light kappa chain

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pHMM

Plasmid

#67717

Purpose

Expression vector MBP/hexahistidine tagged variant of the MS2 coat protein

Depositor

Robert Batey, Jeffrey Kieft

Publication

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Hexahistidine/Maltose binding protein/MS2 coat protein fusion

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7 RNA polymerase

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pKD‐SepRS-EFSep-5x tRNASep (B40 OTS)

Plasmid

#52054

Purpose

Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)

Depositor

Jesse Rinehart

Inserts

SepRS
EF-Sep
5x tRNA Sep

Expression

Bacterial

Mutation

His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

Promoter

Ptac

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pVSV eGFP RABV-G

Plasmid

#31833

Depositor

Connie Cepko

Publication

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

VSV genome

Expression

Bacterial

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pBAD-LSSmOrange

Plasmid

#37129

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

His

Expression

Bacterial

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

araB

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pInt

Plasmid

#18941

Depositor

Michele Calos

Publication

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Expression

Bacterial

Mutation

under lacZ promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

Enlarge
LB322 (Bcl-2 from ATG to -3934)

Plasmid

#15381

Depositor

Linda Boxer

Publication

Heckman et al J Biol Chem. 2000 Mar 3. 275(9):6499-508

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from ATG to -3934 with both P1 and…

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

Enlarge
HLA-A2.1

Plasmid

#14904

Depositor

Linda Sherman

Publication

Irwin et al J Exp Med. 1989 Oct 1. 170(4):1091-101.

Insert

HLA-A2.1 (HLA-A Human)

Expression

Bacterial

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAKgfplux1

Plasmid

#14083

Depositor

Attila Karsi

Publication

Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

Insert

Bacterial luciferase

Expression

Bacterial

Available since

March 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

Enlarge
pGEX-2T-Rac1-wt

Plasmid

#12977

Depositor

Gary Bokoch

Insert

Rac1 (RAC1 Human)

Tags

GST

Expression

Bacterial

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pKM201

Plasmid

#13078

Depositor

Kenan Murphy

Publication

Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

Insert

Ptac-gam-bet-exo operon

Expression

Bacterial

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pVDL9.3

Plasmid

#168299

Purpose

Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

Depositor

Víctor de Lorenzo

Publication

Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

Insert

Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD

Expression

Bacterial

Promoter

LacI-Plac

Available since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pRPN265NB-enh-MNase

Plasmid

#166035

Purpose

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

Depositor

Marc Timmers

Publication

Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

Insert

Glutathione-S-Transferase/enhancer/MNase

Tags

GST

Expression

Bacterial

Promoter

lac promoter / tac promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
COVID-SARS2 NSP13

Plasmid

#159614

Purpose

Bacterial expression plasmid for COVID-SARS2 NSP13 helicase

Depositor

Opher Gileadi

Publication

Newman et al Nat Commun. 2021 Aug 11;12(1):4848. doi:

Insert

Covid-SARS2 Nsp13

Tags

His6-Zb-TEV

Expression

Bacterial

Mutation

Codon-optimized for E. coli expression

Promoter

T7 - LacO

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

Enlarge