30,735 results

1. pCAD29_hIRG1_4-461_pvp008

   Plasmid

   #124843

   Purpose

   Expresses human CAD (Irg1) in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (ACOD1 Human)
   

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-3 and 462-end.

   Promoter

   T7

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_PDCD6_EF-hand

   Plasmid

   #110009

   Purpose

   Protein expression and purification of PDCD6_EF-hand

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PDCD6_EF-hand (PDCD6 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRMT10C (RG9MTD1A-c113)

   Plasmid

   #110248

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   RG9MTD1 (TRMT10C Human)
   

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Promoter

   T7 - lacO

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-pBAD

   Plasmid

   #54630

   Purpose

   Localization: Protein Expression Vector , Excitation: 587, Emission: 610

   Depositor

   Roger Tsien, Nathan Shaner, Michael Davidson

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Type

   Empty backbone

   Tags

   6xHis and mCherry

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKM208

   Plasmid

   #13077

   Depositor

   Kenan Murphy

   Article

   Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

   Insert

   lacI repressor (lacI E. coli)
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTUM4

   Plasmid

   #230905

   Purpose

   Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

   Depositor

   Arne Skerra

   Article

   Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

   Inserts

   FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
   SurA (surA E. coli)
   DsbA (dsbA E. coli)
   DsbC (dsbC E. coli)
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. FASN

   Plasmid

   #38793

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   FASN (FASN Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pUC18T-mini-Tn7T-Gm-Pc-mTurquoise2

   Plasmid

   #111259

   Purpose

   mini-Tn7 vector for insertion of mTurquoise2 (codon optimized for P. fluorescens) at bacterial attTn7 site

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mTurquoise2
   

   Expression

   Bacterial

   Mutation

   mTurquoise2 is codon optimized for expression in …

   Promoter

   Pc

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEVAT7K1A

   Plasmid

   #58302

   Purpose

   GeneGuard vector cassette: thyA, ColE2 COR, Kid toxin; with ampR as cargo.

   Depositor

   Tom Ellis

   Article

   Wright et al ACS Synth Biol. 2014 May 13.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pATetO 6XHis-Taq DNA Ligase

    Plasmid

    #178798

    Purpose

    Inducible expression of Taq DNA Ligase in E. coli

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    Taq DNA Ligase
    

    Expression

    Bacterial

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAIP4_3RZY

    Plasmid

    #234156

    Purpose

    Heterologous protein expression of fatty acid-binding protein, adipocyte (ALBP) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3RZY (FABP4 Human)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. R6K_tac_dGFPmut3_ChInt

    Plasmid

    #187388

    Purpose

    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)

    Depositor

    Roi Avraham

    Insert

    dGFPmut3_AAV
    

    Tags

    AAV

    Expression

    Bacterial

    Mutation

    C-terminal AAV tail

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. BB1_12_pAOX1

    Plasmid

    #98514

    Purpose

    BB1 with insert: promoter PAOX1

    Depositor

    Michael Sauer, Brigitte Gasser, Diethard Mattanovich

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    AOX1 promoter
    

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST14-ARL8B

    Plasmid

    #67383

    Purpose

    Expression of native hArl8b in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8B (ARL8B Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pRAN473

    Plasmid

    #120813

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

    Insert

    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL1

    Plasmid

    #69496

    Purpose

    pJL1 expression vector with sfGFP

    Depositor

    Michael Jewett

    Article

    Martin et al Nat Commun. 2018 Mar 23;9(1):1203. doi:

    Insert

    Super folder GFP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a T7pCONS TIR-2 sfGFP

    Plasmid

    #154464

    Purpose

    pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Tags

    His-thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3xFLAG-CUL4A-pCMV7.1

    Plasmid

    #155022

    Purpose

    N-terminal 3xFLAG-tagged CUL4A in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 4A (CUL4A Human)
    

    Tags

    3xFLAG

    Expression

    Bacterial

    Promoter

    pCMV7.1

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAB46 (CK2beta)

    Plasmid

    #27085

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. human p53-(1-393)

    Plasmid

    #24859

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Full length.

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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