30,875 results

1. pBP-P_cymR-PCymRC

   Plasmid

   #204071

   Purpose

   Type 0 promoter part

   Depositor

   Mikhail Shapiro

   Insert

   cymR repressor and PCymRC cumic acid-inducible promoter
   

   Expression

   Bacterial

   Mutation

   None

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBSSK p85 alpha (delta iSH2)

   Plasmid

   #13432

   Depositor

   Ronald Kahn

   Article

   Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

   Insert

   p85 alpha (delta iSH2) (PIK3R1 Human)
   

   Tags

   flag

   Expression

   Bacterial

   Mutation

   Dominant negative p85 alpha. The p110 alpha bindi…

   Available Since

   Nov. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pOPINTF

   Plasmid

   #41114

   Depositor

   Ray Owens

   Article

   Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

   Type

   Empty backbone

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   March 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQTEV-CIP29

   Plasmid

   #34822

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   cytokine induced protein 29 kDa (SARNP Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDFDuet-trAtCCD7-OsD27(pYL726 )

   Plasmid

   #178285

   Purpose

   Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from Arabidopsis

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Inserts

   OsD27
   trAtCCD7
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a-trAtCCD8(pYL735)

   Plasmid

   #178286

   Purpose

   Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   trAtCCD8
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCX0393

   Plasmid

   #205984

   Purpose

   Ga0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacer

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Inserts

   Ga0116245_10002768 candidate type VII proteins
   Cas14
   Cas5
   truncated CRISPR array
   

   Tags

   14xHis and TwinStrep-tag

   Expression

   Bacterial

   Promoter

   T7 (proteins), pJ23119 (crRNA)

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAKgfplux2

   Plasmid

   #14084

   Depositor

   Attila Karsi

   Article

   Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

   Insert

   Bacterial luciferase-lacIq repressor gene
   

   Expression

   Bacterial

   Available Since

   March 12, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pMSP2

   Plasmid

   #26282

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

   Insert

   MSP2
   

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   synthetic gene of dimer of deletion mutant (1-43)…

   Available Since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

10. OeNL_pRSETb

    Plasmid

    #89529

    Purpose

    Orange color variant of bright luminescent protein for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    OeNL
    

    Tags

    His X 6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBTK569

    Plasmid

    #110614

    Purpose

    BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    E2-Crimson
    

    Expression

    Bacterial

    Mutation

    V32M (see comments below)

    Available Since

    Oct. 2, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. FAK2_HUMAN_D0

    Plasmid

    #79747

    Purpose

    This plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    FAK2 (PTK2B Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pAM232

    Plasmid

    #226654

    Purpose

    pET24b-p97-FLAG

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Tags

    FLAG

    Expression

    Bacterial

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-HemmarG2

    Plasmid

    #112814

    Purpose

    gateway cloning destination vector for expressing enhancer driven CD4-tdGFP

    Depositor

    Chun Han

    Article

    Poe et al Genetics. 2018 Nov 30. pii: genetics.118

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCPP5238

    Plasmid

    #128711

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopG1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDule2-3-nitroTyrosine (5B)

    Plasmid

    #85499

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21-pelB-LaG-42

    Plasmid

    #172758

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-42
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTNSQCas

    Plasmid

    #219434

    Purpose

    CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

    Depositor

    Sheng Yang

    Article

    Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

    Insert

    TnsA, TnsB, TnsC, TniQ, Cas8, Cas7, Cas6
    

    Expression

    Bacterial

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDonor-LoxP

    Plasmid

    #219436

    Purpose

    CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

    Depositor

    Sheng Yang

    Article

    Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

    Insert

    two transposon ends, LoxP-terminator-LoxP gene cassette
    

    Expression

    Bacterial

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSIBR4-Cre

    Plasmid

    #219435

    Purpose

    Theophylline_induced self_splicing intron_based riboswitch for the moderate expression of Cre recombinase

    Depositor

    Sheng Yang

    Article

    Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

    Insert

    intron 4-Cre
    

    Expression

    Bacterial

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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