30,213 results

1. proinsulin-msGFP2 (C48S C70S)

   Plasmid

   #218956

   Purpose

   Expresses proinsulin fused to msGFP2 (C48S C70S) in bacteria

   Depositor

   Benjamin Glick

   Insert

   proinsulin-msGFP2 C48S C70S
   

   Use

   Tags

   proinsulin

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaG-26

   Plasmid

   #172751

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-26
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. IBMc090

   Plasmid

   #161937

   Purpose

   Carries engineered transposon for IBM

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJUMP18-Uac

   Plasmid

   #126959

   Purpose

   Universal Acceptor Plasmid. The fusion sites of the basic part generated are introduced during domestication. sfGFP reporter gene. High copy number oriV #8.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-GST-AGO4

   Plasmid

   #24319

   Depositor

   Carl Novina

   Article

   Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

   Insert

   AGO4 (AGO4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 11, 2011

   Availability

   Academic Institutions and Nonprofits only

6. dCas9-CRY2Clust-FUSN-mut

   Plasmid

   #200970

   Purpose

   induction of phase-separated condensate mediated by FUSN-IDR mutant

   Depositor

   Jian Xu

   Article

   Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

   Insert

   FUSNmut
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   15 Tyr-to-Ser substitutions

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET51b-NAD-cpLuc2

   Plasmid

   #135629

   Purpose

   Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V2)

   Depositor

   Kai Johnsson

   Article

   Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

   Insert

   NAD-cpLuc2 sensor
   

   Use

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-TRE3_ mTFP1- Vamp2

   Plasmid

   #201214

   Purpose

   AAV expression of TRE(TRE3G)-driven, Vamp2-conjugated mTFP1 expression for fluorescent labling of axon terminals

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

   Insert

   mTFP1 (from addgene #54613)
   

   Use

   AAV

   Tags

   mouse Vamp2

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AKAP13

   Plasmid

   #73232

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   AKAP13 (AKAP13 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 1976-2211

   Promoter

   T7

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pBS hBAF57

    Plasmid

    #17877

    Depositor

    Jerry Crabtree

    Article

    Chi et al Nature. 2002 Jul 11. 418(6894):195-9.

    Insert

    BAF57 (SMARCE1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

11. PERK 4: PERKKD-pGEX4T-1

    Plasmid

    #21817

    Depositor

    David Ron

    Article

    Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

    Insert

    PERK (537-1114) (Eif2ak3 Mouse)
    

    Use

    Tags

    GST and Myc (9E10)

    Expression

    Bacterial

    Mutation

    PERK Kinase Domain. Amino acids 537-1114.

    Promoter

    Available since

    March 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLRD04

    Plasmid

    #208258

    Purpose

    Production of ent-kaurene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    ptmT2
    blr2150
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLITMUS*-PrpoN-Cro-PBBa_J23106-geneIII

    Plasmid

    #134353

    Purpose

    Phagemid

    Depositor

    Mark Isalan

    Article

    Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

    Insert

    Cro
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQStrep2-ARPC3

    Plasmid

    #31580

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    Actin related protein 2/3 complex, subunit 3 (ARPC3 Human)
    

    Use

    Tags

    His and StrepII-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOPINO_H3

    Plasmid

    #171927

    Purpose

    Bacterial expression of nanobody VHH_H3, which binds to receptor binding domain of the spike protein of SARS-CoV-2

    Depositor

    Ray Owens

    Article

    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

    Insert

    VHH_H3
    

    Use

    Tags

    His6

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pET3a-His-DJ1

    Plasmid

    #51488

    Purpose

    Inducible expression of human His-DJ1 in E.coli

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 31, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pH3U3-Zif268

    Plasmid

    #12610

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    Zif268 binding site in pH3U3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEMI91

    Plasmid

    #74888

    Purpose

    Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information

    Depositor

    Piotr Marszalek

    Article

    Scholl et al Biomacromolecules. 2016 Jun 8.

    Insert

    Titin I27 x 9 (TTN Human)
    

    Use

    Tags

    His-tag and Strep-tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MBP-FUS_LC_12E

    Plasmid

    #98654

    Purpose

    Expresses MBP-tagged phosphomimetic FUS AA 1-163

    Depositor

    Nicolas Fawzi

    Article

    Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    12 S/T to E

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCPP3417

    Plasmid

    #128724

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopY1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge