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pGEX TIF1gamma

Plasmid

#15734

Depositor

Joan Massague

Publication

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

TIF1 gamma (TRIM33 Human)

Tags

GST

Expression

Bacterial

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pQE9-His-p97(wt)

Plasmid

#14666

Depositor

Graham Warren

Publication

Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

Insert

p97 (Vcp Mouse)

Tags

RGS6xHis

Expression

Bacterial

Mutation

See depositor comments below.

Available since

April 13, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pQLinkHD

Plasmid

#13668

Depositor

Konrad Buessow

Publication

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

Gateway cassette

Use

Co-expression

Tags

His

Expression

Bacterial

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GEV 2

Plasmid

#12616

Depositor

John Louis

Publication

Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

Insert

GB1 domain of protein G as N terminal tag, with IgT antigen as insert (SV40gp6 SV40)

Tags

G protein and His

Expression

Bacterial

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGEX-RIP

Plasmid

#11972

Depositor

Jon Ashwell

Publication

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

RIP (Ripk1 Mouse)

Tags

GST

Expression

Bacterial

Available since

July 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
k3(long) luciferase

Plasmid

#11110

Depositor

Anjana Rao

Publication

Tsai et al Mol Cell Biol. 1996 Feb . 16(2):459-67.

Insert

k3(L) (TNF Human)

Tags

luciferase

Expression

Bacterial

Available since

April 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
Human LFA-1 alpha

Plasmid

#8630

Depositor

Timothy Springer

Publication

Larson et al J Cell Biol 1989 Feb;108(2):703-12.

Insert

LFA1 alpha (ITGAL Human)

Expression

Bacterial

Available since

June 23, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
PARP1 FL

Plasmid

#169815

Purpose

Expresses codon optimised full length His tagged PARP1 in bacterial cells

Depositor

David Neuhaus

Publication

Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

Insert

Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Human, Synthetic)

Tags

MKHHHHHHMKQ

Expression

Bacterial

Mutation

Valine 762 mutated to an alanine

Promoter

T7 promoter

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSL1425 (pSPIN, pCDF backbone)

Plasmid

#160735

Purpose

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

Depositor

Samuel H. Sternberg

Publication

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINO_NbH11-H4

Plasmid

#155364

Purpose

Expresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2

Depositor

Ray Owens

Publication

Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

Insert

H11-H4

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pTOX6

Plasmid

#127451

Purpose

Encodes Tse2 toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Publication

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

tse2 toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30-His-AlaRS

Plasmid

#124103

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Alanyl-tRNA ligase (alaS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET15b-His-RF2

Plasmid

#124130

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Release factor 2 (prfB Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21a-GluRS-His

Plasmid

#124109

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutamyl-tRNA ligase (gltX Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDXinit

Plasmid

#110101

Purpose

E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages

Depositor

Markus Seeger

Publication

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Expression

Bacterial

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pD454-SR mouse Alpha-synuclein

Plasmid

#89075

Purpose

bacterial expression of mouse Alpha-synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

Alpha-synuclein (Snca Mouse)

Expression

Bacterial

Promoter

T7

Available since

March 17, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pUC18T-mini-Tn7T-Gm-ecfp

Plasmid

#65030

Purpose

GmR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

Depositor

Herbert Schweizer

Publication

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

ecfp

Expression

Bacterial

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET-Trx-mSA2

Plasmid

#52320

Purpose

Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

Depositor

Sheldon Park

Publication

Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

Insert

monomeric streptavidin 2

Tags

FLAG, His, S tag, TEV site, and Thioredoxin

Expression

Bacterial

Mutation

S25H mutation compared to streptavidin

Promoter

T7

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBAD/HisB-iRFP720

Plasmid

#45460

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Expression

Bacterial

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

araBAD

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Pet28a_Human_H3.3

Plasmid

#42632

Depositor

Joe Landry

Insert

Human H3.3 (H3-3A Human)

Tags

Untagged

Expression

Bacterial

Promoter

T7

Available since

Feb. 25, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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