30,535 results
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Plasmid#13556DepositorInsertFLIPPi T22A
UseTagsECFP and EYFPExpressionBacterialMutationT22A in binding proteinPromoterAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-Cas9-6xHis
Plasmid#62374PurposeExpression of Cas9-6xHis in bacterial cellsDepositorInsertCas9_6xHis
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a SnoopTag-MBP
Plasmid#72323PurposeBacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.DepositorInsertpET28a SnoopTag-MBP
UseTagsN-terminal His6 tag. MBP is fused at the C-termin…ExpressionBacterialMutationPromoterAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
OaAEP1_C247A
Plasmid#200307PurposeBacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)DepositorInsertAsparaginyl endopeptidase from Oldenlandia affinis
UseTagsSmt3ExpressionBacterialMutationCysteine 247 to AlaninePromoterT7Available SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-3
Plasmid#72678PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAV1K-T5-gfp
Plasmid#26702DepositorInsertT5lacOlacOeGFP biobrick
UseTagsExpressionBacterialMutationPromoterAvailable SinceNov. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET15b His6-FKBPF36V
Plasmid#73180PurposeExpressing FKBPF36V protein in washing out shield-1 ligand for FKBPddDepositorAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
PJEBAN2
Plasmid#203681PurposeExpresses Cyan Fluorescent Protein (CFP) in Listeria monocytogenesDepositorInsertCyan Fluorescent Protein (CFP)
UseTagsExpressionBacterialMutationPromoterAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Frb-v1
Plasmid#201923PurposeE. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.DepositorInsertsFrbA
FrbB
FrbC
FrbD
FrbE
UseTagsExpressionBacterialMutationPromoterT7 (modified), T7 (modified, moderate), T7 (modif…Available SinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRE112
Plasmid#43828DepositorTypeEmpty backboneUseBacterial allelic exchange vector with sacb1TagsExpressionBacterialMutationPromoterAvailable SinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Cdc42
Plasmid#12201DepositorAvailable SinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAB151
Plasmid#101666PurposeOpto-T7RNAP*(563-F1)DepositorInsertOpto-T7RNAP*(563-F1)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-hLIG3
Plasmid#81055Purposebacterial expression of human LIG3DepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minCMV
Plasmid#211518PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSV40 and minCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mTurquoise2-CNA35
Plasmid#61602Purposebacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2DepositorInsertmTurqouise and CNA35
UseTagsHis tagExpressionBacterialMutationPromoterT7Available SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS sfGFP
Plasmid#154462PurposepET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available SinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pScaf
Plasmid#111401PurposePhagemid for producing DNA origami scaffolds, derived from pUC18 and M13mp18DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HaloTag-KanMX6
Plasmid#87029PurposeTag S. pombe genes in C terminal with HaloTagDepositorInsertHaloTag
UseTagsExpressionBacterial and YeastMutationPromoterAvailable SinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-GST
Plasmid#42049DepositorInsertGST
UseTagsHisExpressionBacterialMutationPromoterAvailable SinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only