30,880 results

1. SS13 lac-inducible GFP reporter circuit

   Plasmid

   #30336

   Depositor

   Herbert Sauro

   Article

   Sleight et al J Biol Eng. 2010 Nov 1;4:12

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. EcYbbN pET15b

   Plasmid

   #60690

   Purpose

   Recombinant protein expression of YbbN

   Depositor

   Mark Wilson

   Article

   Lin et al J Biol Chem. 2011 Jun 3;286(22):19459-69

   Insert

   YbbN
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pASPIre3

   Plasmid

   #154844

   Purpose

   Derivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this study

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

   Insert

   Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
   

   Expression

   Bacterial

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTE-10M0X

   Plasmid

   #20318

   Depositor

   Dirk Schnappinger

   Article

   Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

   Insert

   Pimyc-tetR#10
   

   Expression

   Bacterial

   Available Since

   March 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G06_ZIKV NS5

   Plasmid

   #145358

   Purpose

   Cell-free expression vector for Zika virus NS5 protein with C-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS5
   

   Tags

   mCherry and 8xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. proinsulin-moxGFP2

   Plasmid

   #218957

   Purpose

   Expresses proinsulin fused to moxGFP2 in bacteria

   Depositor

   Benjamin Glick

   Insert

   proinsulin-moxGFP2
   

   Tags

   proinsulin

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. proinsulin-mGold

   Plasmid

   #218953

   Purpose

   Expresses proinsulin fused to mGold in bacteria

   Depositor

   Benjamin Glick

   Insert

   proinsulin-mGold
   

   Tags

   proinsulin

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Dox-Akt

   Plasmid

   #207328

   Purpose

   PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)

   Depositor

   Jared Toettcher

   Article

   Underhill et al Development. 2023 Aug 15;150(16):dev2016

   Insert

   rac protein kinase-alpha (AKT1 Human)
   

   Tags

   myristoylation tag - BFP

   Expression

   Bacterial

   Mutation

   range: 129 - 480

   Promoter

   Tet Response Element

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaG-21

   Plasmid

   #172749

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-21
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-pelB-LaG-37

    Plasmid

    #172756

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-37
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET11b-MIF Y99F

    Plasmid

    #149439

    Purpose

    Expression of MIF Y99F in E. coli for protein production

    Depositor

    Elias Lolis

    Insert

    MIF (MIF Human)
    

    Expression

    Bacterial

    Mutation

    Y99F

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSb_init containing the TM287/288 nanobody Nb_TM#2

    Plasmid

    #132698

    Purpose

    expresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    nanobody Nb_TM#2
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEX-Antenna-GDI Cdc42

    Plasmid

    #101681

    Purpose

    "Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al

    Depositor

    Klaus Hahn

    Article

    Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

    Insert

    Cdc42
    

    Tags

    6xHis and FRET Antenna (mCerulean-cpVenus)

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-4T-1 EGFP-Sumo2

    Plasmid

    #13385

    Depositor

    Mary Dasso

    Article

    Azuma et al J Cell Biol. 2003 Nov 10. 163(3):477-87.

    Insert

    Sumo2 (SUMO2 Human)
    

    Tags

    EGFP and GST

    Expression

    Bacterial

    Available Since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pDrop

    Plasmid

    #184872

    Purpose

    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pUS252L

    Plasmid

    #191832

    Purpose

    Expresses fuBFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuBFP to place in other plasmid backbones

    Depositor

    Nicholas Coleman

    Insert

    fuBFP
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pMM555

    Plasmid

    #68880

    Purpose

    NanoLuc expressed constiutively from PcfxA, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Insert

    NanoLuc
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. 4LG1

    Plasmid

    #60092

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    METTL21D:JMC072-C04:C227980 (VCPKMT Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pGEX-ARF

    Plasmid

    #19929

    Depositor

    Yue Xiong

    Article

    Zhang et al Cell. 1998 Mar 20. 92(6):725-34.

    Insert

    cyclin-dependent kinase inhibitor 2A p19ARF (CDKN2A Human)
    

    Expression

    Bacterial

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)

    Plasmid

    #191650

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletion

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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