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pST39-Ubc13-MMS2

Plasmid

#184807

Purpose

Bacterial expression of yeast UEV-E2 complex

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

Ubc13, MMS2

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD-4D

Plasmid

#178672

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with four Asp residues removed (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-4D (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

four Asp residues removed

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5DL491-492

Plasmid

#198290

Purpose

Produces PVCs retargeted with non-binding mutant of Ad5 knob

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5DL491-492

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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PatoL-OG241

Plasmid

#72841

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoL

Expression

Bacterial

Promoter

PatoL

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX Stat5B-SH2

Plasmid

#46516

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat5B (STAT5B Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 593-692

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pMS30: pTarget(PmcCAST)

Plasmid

#168163

Purpose

Target plasmid containing PmcPSP1 and attachment site for PmcCAST.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PAM for PmcCAST + PmcPSP1
tRNA-Val

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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htpG2-GFP

Plasmid

#109388

Purpose

GFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

phtpG2-GFP

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLZL2386

Plasmid

#48682

Purpose

pET29a_PPP CODA PFV Integrase with C-terminal His tag (GPG removed from pLZL2373)

Depositor

Suzanne Sandmeyer

Article

Qi et al PLoS One. 2013 May 17;8(5):e63957. doi:

Insert

PFV Integrase

Tags

His

Expression

Bacterial

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pBBR6k-J23101-GFPuv

Plasmid

#106390

Purpose

pBBR6k-GFPuv with Anderson promoter J23101 expressing lacI

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

LlacO-1

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pOPINTF

Plasmid

#41114

Depositor

Ray Owens

Article

Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

Type

Empty backbone

Expression

Bacterial, Insect, and Mamm…

Available Since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

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pCCL-ReDNAP (Y427A)

Plasmid

#52346

Purpose

CEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)

Depositor

Chang Liu

Article

Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

Insert

Recoded TP-DNAP

Expression

Bacterial and Yeast

Promoter

REV1

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCPP5951

Plasmid

#128719

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

Insert

hopR1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET19b+H2B

Plasmid

#157809

Purpose

Expression of human histone H2B

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H2B

Expression

Bacterial

Promoter

T7

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMG81-MBP-t-M.MpeI-N374K-His

Plasmid

#197985

Purpose

Expresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag.

Depositor

Rahul Kohli

Article

Wang et al Nat Chem Biol. 2023 Jun 15. doi: 10.1038

Insert

MBP-t-M.MpeI-N374K-His

Tags

His and MBP

Expression

Bacterial

Mutation

M.MpeI-N374K

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pSALECTNK-TEM1(S70A,D179G)/csTEM1

Plasmid

#81163

Purpose

pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

TEM-1 beta-lactamase

Tags

Codon swapped TEM-1 from H26 to W290 and His6x

Expression

Bacterial

Mutation

Deleted the sec transport tag. Plasmid encodes H2…

Promoter

lac promoter

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pEE060

Plasmid

#176817

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

611

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET32a-hEPR

Plasmid

#199233

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Proepiregulin preproprotein (EREG Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pKB232

Plasmid

#170019

Purpose

Encodes LacI-controlled expression of surface-displayed murine CRAMP and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
CRAMP

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_TXN_Thioredoxin

Plasmid

#109778

Purpose

Protein expression and purification of TXN_Thioredoxin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

TXN_Thioredoxin (TXN Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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His-TEV-Aurein1.2_(+36GFP)-LPETG-His

Plasmid

#71755

Purpose

His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

Aurein1.2_(pos36_GFP)-LPETG-His

Tags

6XHis, Sortase (LPETG), and TEV Protease

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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