30,535 results

1. pMVV052

   Plasmid

   #215303

   Purpose

   Expresses sfGFP under rhamnose induction (RhaS + RhaR + pRhaB-sfGFP) with a BBR1 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   PRhaB

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pnCas9-AID

   Plasmid

   #196242

   Purpose

   Plasmid expressing the nCas9-AID fusion

   Depositor

   Andrea Puhar

   Article

   Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

   Insert

   nickase Cas9 fused to AID
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-Expotin 1

   Plasmid

   #42047

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   Exportin-1 (XPO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRKH3-slpGFP

   Plasmid

   #27168

   Depositor

   Michela Lizier

   Article

   Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

   Insert

   surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFPC1-EPSIN 1

   Plasmid

   #22228

   Depositor

   Pietro De Camilli

   Article

   Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

   Insert

   Epsin 1 (Epn1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   please see depositor comment below

   Promoter

   Available Since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSF1389

   Plasmid

   #104962

   Purpose

   Bacterial expression plasmid of bdSENP1 protease

   Depositor

   Dirk Görlich

   Article

   Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

   Insert

   SENP1
   

   Use

   Tags

   His14 tag and Tev protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available Since

   Jan. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSecUAG-Evol2

   Plasmid

   #163148

   Purpose

   Expresses machinery necessary for selenocysteine incorporation at UAG codons

   Depositor

   Dieter Söll

   Article

   Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2

   Inserts

   allo-tRNA UTu2D
   thioredoxin
   Selenophosphate synthase
   Selenocysteine synthase
   Cysteine desulfurase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cysteine 32 switched to Selenocysteine (UAG), Cys…

   Promoter

   EM7, araBAD, native As SelD promoter, and native …

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH6HTC_PRDX6-Halo

   Plasmid

   #175338

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   PRDX6 (PRDX6 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAB213

   Plasmid

   #101677

   Purpose

   mCherry under T7 promoter control

   Depositor

   Mustafa Khammash

   Article

   Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

   Insert

   mCherry under T7 promoter control
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-dCas9-VP64-6xHis

    Plasmid

    #62935

    Purpose

    Expression of dCas9-VP64-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    dCas9-VP64
    

    Use

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A and H840A amino acid changes render Cas9 nuc…

    Promoter

    T7

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid

    #112203

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

    Depositor

    Christopher Johnson, Gregg Beckham

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    

    Use

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    W159H, S238F

    Promoter

    N/A

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSP3545

    Plasmid

    #46888

    Purpose

    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

    Depositor

    Gary Dunny

    Article

    Bryan et al Plasmid. 2000 Sep;44(2):183-90.

    Type

    Empty backbone

    Use

    Nisin-inducible expression; gram-positive bacteri…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PnisA

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7NT*-Bri2 113-231 R221E

    Plasmid

    #138134

    Purpose

    Expresseds human Bri2 BRICHOS R221E mutant in E. coli

    Depositor

    Jan Johansson

    Article

    Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

    Insert

    human Bri2 BRICHOS R221E (ITM2B Human)
    

    Use

    Tags

    NT* tag derived from spider silk proteins

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CeNL_pRSETb

    Plasmid

    #89510

    Purpose

    Cyan color variant of bright luminescent protein for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Use

    Tags

    His X 6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJ4M/TDP-43

    Plasmid

    #104480

    Purpose

    bacterial expression of TDP-43

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. +36GFP-LPETG

    Plasmid

    #71754

    Purpose

    +36GFP-LPETG for expression in E.Coli

    Depositor

    David Liu

    Article

    Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

    Insert

    (GGS)9_(pos36GFP)_6XHis_LPETG
    

    Use

    Tags

    6XHis and Sortase (LPETG)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDB179-HotPETase

    Plasmid

    #218701

    Purpose

    Encodes codon optimized HotPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    HotPETase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21a(+)-HIV RT-6xHis-RBS-prot

    Plasmid

    #159149

    Purpose

    expresses His-tagged HIV reverse transcriptase and untagged HIV protease

    Depositor

    Andrea Pauli

    Article

    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

    Inserts

    Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
    Human immunodeficiency virus 1 (HIV-1) protease
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    HIV-1 group M/subtype B – BH10 strain

    Promoter

    Available Since

    Sept. 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pOSIP-KH (KanR, HK022)

    Plasmid

    #45983

    Depositor

    Keith Shearwin, Drew Endy

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVoPo-GFP

    Plasmid

    #187840

    Purpose

    Constitutive GFP-expression vector for Fusobacterium nucleatum

    Depositor

    Jörg Vogel

    Article

    Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge