32,684 results
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Plasmid#15924DepositorAvailable SinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only
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pET11_Ec_ORF209
Plasmid#69029PurposeExpresses E.coli ORF209/ADPRase in bacterial cellsDepositorInsertORF209/ADPRase
Tags-ExpressionBacterialAvailable SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
KIF3A406
Plasmid#129768PurposeExpresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assaysDepositorInsertKIF3A (Kif3a Mouse)
TagsHis6ExpressionBacterialMutationTruncation at 359, fused to kinesin-1 starting Al…Available SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEVA12TEC-I
Plasmid#58308PurposeGeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z.DepositorInsertGenomic cassette
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
E. coli expression: Z-lock cofilin dark state mutant
Plasmid#141135Purposea control for optogenetic cofilin analogDepositorInsertZdk2, Cofilin, LOV2
ExpressionBacterialMutationD3 deletion, I32F in Zdk2Available SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU1100
Plasmid#14465DepositorInsertunstable gfp-mut3.1 LVA
ExpressionBacterialAvailable SinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-PSPH
Plasmid#83909Purposestable overexpressionDepositorAvailable SinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFREE2-SpCas9-sgRNA
Plasmid#179582PurposeExpresses spCas9 and gRNA that targets ColE1 plasmids for curingDepositorInsertCas9
ExpressionBacterialAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET_BGL2
Plasmid#101911PurposeExpresses a β-glucosidase gene from Thermobaculum terrenum in E. coli under the control of the pET system.DepositorInsertβ-glucosidase
UseSynthetic BiologyExpressionBacterialPromoterT7+LacOAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rhadCas9-native
Plasmid#129392PurposeDerived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.DepositorInsertS. pyogenes dCas9
UseCRISPRExpressionBacterialMutationAspartate 10 to Alanine, Histidine 840 to AlaninePromoterPrhaBADAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTE103
Plasmid#59398PurposeMethyloBrick expression vector, fumC promoter; TcRDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPfumCAvailable SinceDec. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRC3EGFP
Plasmid#193790PurposeExpresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertEGFP
ExpressionBacterialPromoterPR promoterAvailable SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX_hDDX5_FL_D248N
Plasmid#88872Purposeinducible expression of GST-DDX5 D248N in bacteriaDepositorAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
PET-3a-chpBK
Plasmid#155201PurposeTo express recombinant ChpBK proteinDepositorInsertschpS-chpBK-6×His-Tag
GyrA-CBD-10xHis-Tag
Tags10xHis and 6xHisExpressionBacterialAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJKR-L-cdaR
Plasmid#62558PurposeGlucarate biosensor. Low copy plasmid that expresses GFP proportional to glucarate concentration.DepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET14b PHAS-I
Plasmid#15679DepositorInsertPHAS-I (Eif4ebp1 Rat)
Tags6xHisExpressionBacterialMutationNucleotides 1-354 of rat PHAS-I plus 450bp of 3…Available SinceSept. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHBJT001
Plasmid#225152PurposeHigh copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_13
Plasmid#194674PurposeProtein production of the split HaloTag based calcium recorder Caprola_13 in bacteriaDepositorInsertCaprola_13
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDL17_Cas9-His Δcys M1C
Plasmid#206251PurposeBacterial expression of SpCas9 Δcys M1C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationM1C, C80S, C574SPromoterT7Available SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
His Puc (aa 1-393)
Plasmid#52295PurposeBacterial expression of His tagged Drosophila Puc fragmentDepositorAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only