30,810 results

1. pET-CV-Ykt6

   Plasmid

   #168030

   Purpose

   Expresses eCFP-Ykt6(Q131-K190)-mVenus in bacterial cells

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   eCFP-Ykt6(Q131-K190)-mVenus
   

   Tags

   Hisx6

   Expression

   Bacterial

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSP2253

   Plasmid

   #70704

   Purpose

   Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-4T-1 3xHA-MKK4-EE

   Plasmid

   #47577

   Purpose

   Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   MKK4-EE (MAP2K4 Human)
   

   Tags

   3xHA and GST

   Expression

   Bacterial

   Mutation

   Lacks the first 36 residues of the RefSeq protein…

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCDF-mcherry2

   Plasmid

   #87145

   Purpose

   Constitutive E. coli mcherry Expression, with DAS4 tag

   Depositor

   Michael Lynch

   Insert

   mcherry
   

   Tags

   C terminal DAS4 tag

   Expression

   Bacterial

   Promoter

   Constitutive proD

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. REM2

   Plasmid

   #55682

   Purpose

   expression of mouse REM2

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   REM2 (Rem2 Mouse)
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pRM2

   Plasmid

   #8486

   Depositor

   Nancy Craig

   Article

   McKown et al J Bacteriol 1988 Jan;170(1):352-8.

   Insert

   attTn7 (tnsA Bacteria)
   

   Expression

   Bacterial

   Available Since

   Dec. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRE163

   Plasmid

   #118511

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sYFP2
   

   Expression

   Bacterial

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. R701-X55-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12V

   Plasmid

   #159563

   Purpose

   E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12V

   Depositor

   Dominic Esposito

   Article

   Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

   Insert

   His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12V (KRAS Human)
   

   Tags

   His6-MBP-tev-Avi

   Expression

   Bacterial

   Mutation

   G12V

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOD3-proD/mNeptune2-nat

   Plasmid

   #120337

   Purpose

   Bacterial mNeptune2 labeling

   Depositor

   James Kirby

   Article

   Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

   Insert

   mNeptune2
   

   Expression

   Bacterial

   Promoter

   proD

   Available Since

   Aug. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. UIMLx2

    Plasmid

    #174888

    Purpose

    UIMLx2 ubiquitin probe for K48 ubiquitylated proteins

    Depositor

    Peter Kaiser

    Article

    Villamil et al Mol Cell Proteomics. 2022 Jan;21(1):1001

    Insert

    Met4 UIML
    

    Tags

    Avi-6xHis-Smt3

    Expression

    Bacterial

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC-EsaR-D91G

    Plasmid

    #47646

    Purpose

    pAC-EsaR with a a272g mutation in esaR

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    esaR-D91G
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 91 to Glycine

    Promoter

    Plac

    Available Since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pEB2-TagRFP

    Plasmid

    #103997

    Purpose

    Plasmid encoding TagRFP

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    TagRFP
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, K67R, …

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJMP1104

    Plasmid

    #119248

    Purpose

    knockdown pqsC in P. aeruginosa

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA pqsC (P. aeruginosa)
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pgRNA-non-target

    Plasmid

    #129465

    Purpose

    Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Non-target/random gRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    BBa_J23119 (SpeI)

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTXB1-H2B(1-114)_wt

    Plasmid

    #118252

    Purpose

    Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    xlH2B(1-114)
    

    Tags

    Mxe-GyrA intein fused to chitin binding domain

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRE-Tn7-153

    Plasmid

    #118567

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sYFP2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSB1K3_pBen-sfGFP_J23101-mRFP

    Plasmid

    #128126

    Purpose

    Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pBen (benzoate responsive promoter)

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS hTSP1

    Plasmid

    #12410

    Depositor

    Paul Bornstein

    Insert

    TSP1 (THBS1 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 23, 2006

    Availability

    Academic Institutions and Nonprofits only

19. GFP S2TAG/E17TAG

    Plasmid

    #68298

    Purpose

    S2TAG/E17TAG GFP reporter

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    GFP S2TAG/E17TAG
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET23a RanGAP

    Plasmid

    #13387

    Depositor

    Mary Dasso

    Article

    Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

    Insert

    RanGAP (RANGAP1 Human)
    

    Expression

    Bacterial

    Available Since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only