32,771 results
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Plasmid#208066Purposeexpression of human Replication Protein A in E. coliDepositorExpressionBacterialMutationRPA32 has a point mutation Thr88(ACC) -> Ile(A…Available SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only
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pT7II-1N4Rtau
Plasmid#249554PurposeRecombinant protein synthesis of 1N4R isoform of human TauDepositorInsertTau (1N4R) (MAPT Human)
ExpressionBacterialAvailable SinceJan. 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-HIV RT-6xHis-RBS-prot
Plasmid#159149Purposeexpresses His-tagged HIV reverse transcriptase and untagged HIV proteaseDepositorInsertsHuman immunodeficiency virus 1 (HIV-1) reverse transcriptase
Human immunodeficiency virus 1 (HIV-1) protease
TagsHisExpressionBacterialMutationHIV-1 group M/subtype B – BH10 strainAvailable SinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Butelase AY
Plasmid#221847PurposeExpresses Butelase AY with Val237Ala and Thr238Tyr mutationsDepositorInsertButelase 1 ligase
ExpressionBacterialMutationVal237Ala, Thr238TyrAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-Ad5_HUHe-Pvc10-Cas9
Plasmid#243744PurposePVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with HUHe on NTD and Cas9 on CTDDepositorInsertpvc13-Ad5_HUHe-Pvc10-Cas9
ExpressionBacterialAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-GFPuv
Plasmid#127078PurposeLow phosphate inducible yibD gene promoter expressing GFPuv reporterDepositorInsertGFPuv
ExpressionBacterialPromoteryibDpAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterNative promoter from pMUT2 relB/relE operon and P…Available SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMJ922
Plasmid#78312PurposeExpression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coliDepositorInsertSpCas9
TagsHA-2xNLS-EGFP-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-FBXL5-SKP1
Plasmid#226620PurposeCo-expression of FBXL5-SKP1 complex in E.coliDepositorTagsGB1ExpressionBacterialMutationAmino acids 1-198 was deleted, and 420-596 was re…PromoterT7Available SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTCF158 Human beta-2 microglobulin
Plasmid#180457PurposeE. coli expression of class I MHC light chainDepositorInsertHuman beta-2 microglobulin
ExpressionBacterialAvailable SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGM1192
Plasmid#115683PurposeE. coli-Streptomyces shuttle vector for gene expressionDepositorInsertmCherry, aac(3)IV, tsr
TagsStrep-tagII on mCherry, codon optimizedExpressionBacterialMutationmCherry codon optimized for StreptomycesPromoterSF14*Available SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAJF1131
Plasmid#252906PurposeT7-pelB-NBALFA-LE-6his amp (for purification of his-tagged NBALFA from E. coli)DepositorInsertNBALFA
TagsHis Tag and pelB leaderExpressionBacterialPromoterT7Available SinceMarch 30, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-sfGFP
Plasmid#199248PurposeFluorescent labelling of bacterial cells with sfGFPDepositorInsertCodon-optimized sfGFP gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
PEP119T
Plasmid#98251PurposePseudomonas T3SS gateway vector with HA-sfGFP11-sfGFP11DepositorInsertGateway cassettee-HA-sfGFP11-11
ExpressionBacterialAvailable SinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p
Plasmid#202589PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialAvailable SinceJune 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-eyfp
Plasmid#65031PurposeGmR mini-Tn7 vector, mobilizable, for YFP tagging bacteriaDepositorInserteyfp
ExpressionBacterialAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMevT
Plasmid#17815DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
p11d-aRPA (MSW#712)
Plasmid#208078PurposeExpression of Alternative human Replication Protein A (RPA1,4,3) in E. coliDepositorAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp6-His
Plasmid#11823DepositorAvailable SinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)
Plasmid#191801PurposeFull length wt AtPCO4 with Nt-His tagDepositorInsertAtPCO4-1
Tags6xHis, TEV cleavage siteExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pR540
Plasmid#207885Purposetemplate for 2 gRNA amplificationDepositorInsertscaffold-pU6
ExpressionBacterialAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-Tn7-PcL
Plasmid#225281PurposeMini-Tn7 pGP-Tn7-Cm vector with constitutive PcL promoter for the constitutive expression of target genes inserted into the attTn7 chromosomal site.DepositorTypeEmpty backboneExpressionBacterialPromoterConstitutive PcL promoterAvailable SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD-bHs
Plasmid#134259PurposeFor L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tagDepositorInsertHyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
Tags6x HisExpressionBacterialMutationcodon optimized for Salmonella typhimuriumPromoterAraCAvailable SinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
JMJD1BA-c068
Plasmid#53627Purposebacterial expression plasmidDepositorInsertJMJD1BA
TagsTEV-His6-FlagExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.0-Pct5.1-crRNA(AarI)
Plasmid#191633PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloningDepositorInsertCymR
UseCRISPRExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.b201
Plasmid#131404PurposeE. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albuminDepositorInsertNanobody b201
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available SinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD02-SsMenshen
Plasmid#254577PurposeFor the expression of the Menshen system encoded by Solibacillus silvestris StLB046DepositorInsertSsNsnA, SsNsnB, SsNsnC
ExpressionBacterialAvailable SinceMay 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBAD02-EcMenshen
Plasmid#254561PurposeFor the expression of the Menshen system encoded by Escherichia coli 2-177-06_S3_C1DepositorInsertEcNsnA, EcNsnB, EcNsnC
ExpressionBacterialAvailable SinceMay 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLann-HRV-3C
Plasmid#254756PurposeExpress Prescission ProteaseDepositorInsertHRV-3C
TagsGSTExpressionBacterialAvailable SinceApril 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pGLU_504
Plasmid#250498PurposeUrease Cluster KO | Erythromycin Resistance Cassette | Conjugative VectorDepositorInsertsermC
ermC
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 23, 2026AvailabilityAcademic Institutions and Nonprofits only