32,967 results
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Plasmid#220194Purposemini-Tn7 dCas9 expression vector - LacI/pLac promoterDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterLacI/pLacAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluh
Plasmid#119830PurposeProduction of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluh
TagsGSTExpressionBacterialMutationE25APromoterT7Available SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
SoxS-GFP
Plasmid#220165PurposeTo track the natural SoxS promoter's transcriptional dynamicsDepositorInsertSoxS promoter only
ExpressionBacterialAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-D-TOPO-PARP14-SΔK
Plasmid#216658PurposeExpresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.DepositorInsertPARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
Tags6xHis-V5-TEVExpressionBacterialMutationDeletion starting from Q1390 to D1448 and substit…PromoterT7Available SinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET mNG-GECO1
Plasmid#201933PurposeExpresses mNG-GECO1 indicator in bacteriaDepositorInsertmNeonGreenGECO1
TagsHIS tagExpressionBacterialAvailable SinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC3-FGFR1
Plasmid#222611PurposeExpression of MAC3-tagged FGFR1DepositorAvailable SinceAug. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC3-EGFR
Plasmid#222610PurposeExpression of MAC3-tagged EGFRDepositorAvailable SinceAug. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MUNC18a
Plasmid#218888PurposeExpresses rat MUNC18a in bacteriaDepositorAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
RepairTemplate_NIPBL_mEGFP_mAID
Plasmid#217664PurposeRepair template to endogenously tag NIPBL with mEGFP-mAID TagDepositorAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-C-MBP
Plasmid#214304PurposeProvide a bacterial expression vector that encodes C-terminus MBP tagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
His_CL7_2NLS_iCas12a-4NLS
Plasmid#222971PurposeBacterial expression of NLS-rich iCas12a constructDepositorInsertHis_CL7_2NLS_iCas12a-4NLS
UseCRISPRTagsCL7 and HisExpressionBacterialPromotertacAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-TEV-HsFbxo7-129-398/Skp1
Plasmid#202592PurposeBacterial expression of HsFbxo7-129-398 and HsSkp1DepositorTagsGST, TEVExpressionBacterialMutationaa 129-398 onlyAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3079 (p7INT-Pveg_lacI)
Plasmid#218505Purposeintegrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integraseDepositorAvailable SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mScarlet-TEV-Strep-His
Plasmid#210823PurposeBacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent proteinDepositorInsertmScarlet
TagsTwinStrep, 10xHisExpressionBacterialPromoterT7Available SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQ-Elo-HB-C-A(1-630)
Plasmid#171086PurposeCo-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOBDepositorTagsHisExpressionBacterialMutationDeleted N-term 26aa and C-term 142aa for mutant e…PromoterT5 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-TFIIE
Plasmid#171083PurposeCo-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permissionDepositorTagsHisExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQ-Elo-HB-C-A(1-772)
Plasmid#171085PurposeCo-expresses of WT-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOBDepositorTagsHisExpressionBacterialMutationDeleted N-term 26 amino acids for expression purp…PromoterT5 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
p1153
Plasmid#222930PurposeShuttle vector carrying a Candida auris centromere (CEN 4) with Nourseothricin selectable markerDepositorInsertCEN 4
ExpressionBacterialAvailable SinceJuly 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJMP3667
Plasmid#222353PurposeExpression vector with GFP expressed under PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli.DepositorInsertsfGFP
ExpressionBacterialPromoterPabstBRAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only