30,736 results

1. pSW002-Pc-mNeonGreen

   Plasmid

   #205016

   Purpose

   Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mNeonGreen
   

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   Pc

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCM433kanT

   Plasmid

   #61262

   Purpose

   Sucrose counterselection (sacB) vector for unmarked allelic exchange.

   Depositor

   Mary Lidstrom

   Article

   Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

   Type

   Empty backbone

   Use

   Synthetic Biology; Sucrose counterselection

   Expression

   Bacterial

   Available Since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTW334e

   Plasmid

   #237315

   Purpose

   expresses A. thaliana Cpn60α Cpn60β Cpn20 Raf1 Raf2 RbcX Bsd2

   Depositor

   Tina Wang

   Article

   Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

   Insert

   A. thaliana Cpn60α Cpn60β Cpn20 Raf1 Raf2 RbcX Bsd2
   

   Expression

   Bacterial

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-6P1-hPXR-LBD

   Plasmid

   #67769

   Purpose

   Expression GST-hPXR-LBD in bacteria

   Depositor

   Sridhar Mani

   Article

   Li et al J Biol Chem. 2013 May 10;288(19):13655-6

   Insert

   human pregnane X receptor (PXR) ligand binding domain (NR1I2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains amino acids 107 to 434 of human PXR

   Promoter

   Tac

   Available Since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSW002-PpsbA_mTurquoise2

   Plasmid

   #108237

   Purpose

   Broad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   PsbA promoter - mTurquoise2
   

   Expression

   Bacterial

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-6xHis-MBP-TEV site-TadA8.20

   Plasmid

   #194702

   Purpose

   Expresses N-terminal MBP fused TadA8.20 in bacteria

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   TadA8.20
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   mutations in TadA are described in the paper

   Promoter

   T7 promoter

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-Padron2

   Plasmid

   #172355

   Purpose

   Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

   Depositor

   Stefan Jakobs

   Article

   Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

   Insert

   Padron2
   

   Tags

   6xHisTag (N terminal on backbone)

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdCRISPomyces-2BD

   Plasmid

   #234429

   Purpose

   Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template

   Depositor

   Min-Kyu Oh

   Article

   Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

   Insert

   Modified dCas9(D10A, H840A) with polyaspartate linking
   

   Expression

   Bacterial

   Mutation

   Linked polyaspartate using glycine-serine linker

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTYB3-T4gp43(WT)

   Plasmid

   #228203

   Purpose

   Expression in BL21(DE3) of WT T4 DNA polymerase

   Depositor

   Stephen Benkovic

   Insert

   43
   

   Tags

   Sce VMA intein-chitin binding domain

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSW036

    Plasmid

    #140034

    Purpose

    KO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoter

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Yellow fluorescent protein
    

    Expression

    Bacterial

    Promoter

    cpt

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2+Paxillin-mKate

    Plasmid

    #105974

    Purpose

    in vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesions

    Depositor

    Caren Norden

    Article

    Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

    Insert

    Paxillin
    

    Tags

    mKate

    Expression

    Bacterial

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD33-mf-lon

    Plasmid

    #21867

    Depositor

    Robert Sauer

    Article

    Gur et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

    Insert

    Lon (Mfl404 Mesoplasma florum)
    

    Expression

    Bacterial

    Mutation

    all TGA codons changed to TGG codons

    Available Since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD-HisD-mNG-Ec-Kbp-D9N

    Plasmid

    #178011

    Purpose

    Bacterial expression of green fluorescent potassium indicator mNG-Ec-Kbp-D9N (KRaION1 mutant D9N)

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    mNG-Ec-Kbp-D9N
    

    Expression

    Bacterial

    Promoter

    araB

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBJ6

    Plasmid

    #167135

    Purpose

    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    Kanamycin promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pULTRA-CNF

    Plasmid

    #48215

    Purpose

    highly efficient suppressor plasmid

    Depositor

    Peter Schultz

    Article

    Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-

    Inserts

    polyspecific MJ tyrosyl synthetase
    tRNA
    

    Expression

    Bacterial

    Mutation

    Y32L, L65V, F108W, Q109M, D158G, I159A

    Promoter

    proK and tacI

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGST-PSP-hTG

    Plasmid

    #85470

    Purpose

    bacterial expression of human tissue transglutaminase

    Depositor

    Jeffrey Keillor, Ray Truant

    Article

    Roy et al Protein Expr Purif. 2013 Jan;87(1):41-6.

    Insert

    hTG2 (TGM2 Human)
    

    Tags

    GST and PSP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas9

    Plasmid

    #167547

    Purpose

    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Inserts

    chloramphenicol acetyl transferase
    pUC19 origin of replication
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFPC1-EPSIN 2

    Plasmid

    #22242

    Depositor

    Pietro De Camilli

    Article

    Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

    Insert

    Epsin 2 (EPN2 Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pET-NtLNtC60αC20C60β

    Plasmid

    #160874

    Purpose

    Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Expression

    Bacterial

    Promoter

    rbcL: pBAD, chaperones: T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. mVenus-pBAD

    Plasmid

    #54845

    Purpose

    Localization: Protein Expression Vector , Excitation: 515, Emission: 528

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

    Type

    Empty backbone

    Tags

    6xHis and mVenus

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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