31,059 results
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Plasmid#175458PurposeProtein expression in bacterial cells. Entire CDS, M1-D1087.DepositorAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only
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pRAD1
Plasmid#47697PurposeGeneral cloning vector for use in E. coli or D. radioduransDepositorTypeEmpty backboneExpressionBacterialPromoterrepUAvailable SinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MBP-His-SFP
Plasmid#141141PurposeExpression of 4'-phosphopantetheinyl transferase geneDepositorInsert4'-phosphopantetheinyl transferase
TagsMBPExpressionBacterialAvailable SinceMay 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-PP7v3
Plasmid#107230PurposeExpresses 24x optimized PP7 stem loops (version 3) in bacterial cellsDepositorInsert24xPP7v3
Tags3x FLAG-tag and P2A siteExpressionBacterialAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-2-human RNF146 full length
Plasmid#132610Purposeexpresses human RNF146 full length in E. coliDepositorInsertRNF146 (RNF146 Human)
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
NSUN5
Plasmid#25491PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZac2.1 gfaABC1D-Cx43-BioID2-BioID2-HA
Plasmid#176743PurposeExpresses Connexin-43 fused with BioID2 at the C-terminus in astrocytesDepositorInsertCx43-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEGF
Plasmid#199231PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertEpidermal growth factor, partial (EGF Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDZ617 pKAN 12xPP7 V4
Plasmid#72237PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interestDepositorInsert12xPP7 V4
UseCre/LoxExpressionBacterialAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-TEV-C9R
Plasmid#118754PurposeTEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activityDepositorInsertTobacco Etch Virus protease (NEWENTRY Synthetic)
TagsC9R and His tagExpressionBacterialPromoterT7Available SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL004
Plasmid#215336PurposeamyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)DepositorTypeEmpty backboneExpressionBacterialPromoterhyper-pSpankAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSecUAG-Evol2
Plasmid#163148PurposeExpresses machinery necessary for selenocysteine incorporation at UAG codonsDepositorInsertsallo-tRNA UTu2D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
Cysteine desulfurase
ExpressionBacterialMutationCysteine 32 switched to Selenocysteine (UAG), Cys…PromoterEM7, araBAD, native As SelD promoter, and native …Available SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCE1
Plasmid#174434PurposeCandida optimized monomeric eGFP with RIPLING linker sequence.DepositorInsertCandida optimized monomeric GFP
ExpressionBacterialAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a_TIA1
Plasmid#106095Purposebacterial protein expression of TIA1DepositorAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET-4xTR-TUBE
Plasmid#110312PurposeE. coli expression and purification of 4xTR-TUBEDepositorInsert4xTR-TUBE (UBQLN1 Human)
TagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQTEV- PRDX2
Plasmid#31338DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-gp32-H6
Plasmid#163912PurposeExpresses T4 gp32 for bacterial expression and affinity purificationDepositorInsertgp32
TagsHisExpressionBacterialPromoterT7 promoterAvailable SinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCold TDP43 WT
Plasmid#27451DepositorAvailable SinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHIS-GB1-BP1
Plasmid#133427PurposeHuman BP1 fragment of SREBF1 with GB1 solubility tag in vector for in vitro expression, with T7 promoter.DepositorInsertSREBF1 (SREBF1 Human)
ExpressionBacterialMutationSREBP1a amino acids 17-42 fused to GB1 solubilit…Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWLlR5
Plasmid#149492PurposeBroad-Host range expression vector, incW (pSa) origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLlacO-1Available SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only