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GST-TACC3-His6

Plasmid

#69106

Purpose

For bacterial expression of recombinant human TACC3 tagged with GST and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Tags

GST and His6

Expression

Bacterial

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pETDuet-LahT150

Plasmid

#166785

Purpose

Cleavage of double glycine motif in peptides to remove the leader sequence

Depositor

Wilfred van der Donk

Article

Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

Insert

LahT150

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b_H6-Smt3-AesC-GFP

Plasmid

#234486

Purpose

Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

Depositor

Henning Mootz

Article

Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

Insert

C-terminal Fragment of the Aes123 PolB1 Split Intein

Tags

Hexahistidine Tag, Superfolder Green Fluorescent …

Expression

Bacterial

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCF1135

Plasmid

#222106

Purpose

E. coli - Veillonella shuttle plasmid with single selectable marker

Depositor

J Christopher Fenno

Article

Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

Type

Empty backbone

Use

E. coli-veillonella shuttle plasmid

Expression

Bacterial

Available Since

Oct. 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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p15TV-L

Plasmid

#26093

Purpose

SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Tags

6xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Sept. 6, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pMT416

Plasmid

#8607

Depositor

Robert Hartley

Article

Hartley et al J Mol Biol 1988 Aug 20;202(4):913-5.

Insert

barnase, barstar

Tags

phoA signal sequence to barnase

Expression

Bacterial

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pETDuet-1 TIM9,10

Plasmid

#170280

Purpose

Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

Depositor

Paul Schanda

Article

Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.

Insert

mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)

Expression

Bacterial

Promoter

Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDae079 (eMutaT7[transition])

Plasmid

#187622

Purpose

Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Inserts

TadA-8e-T7RNAP
PmCDA1-T7RNAP

Expression

Bacterial

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCWU6-xylR-E-luciferase

Plasmid

#190721

Purpose

fine tune expression of luciferase under the control of xylose- and theophylline- inducible system

Depositor

Chenggang Wu

Article

Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

Insert

xylR-E-luciferase

Use

Luciferase

Expression

Bacterial

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMD19-PBBa_J23117-sgRNA-Spr

Plasmid

#190793

Purpose

Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

Depositor

Paul Hudson

Article

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Insert

sgRNA (dummy)

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23117

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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SQ171fg/pRibo-T

Plasmid

#69343

Purpose

SQ171 fast growing strain supported by pRibo-T plasmid

Depositor

Michael Jewett

Article

Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

Insert

Ribo-T

Expression

Bacterial

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRM049 His-TEV-Cas9 triNLS 2C

Plasmid

#196245

Purpose

Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Article

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Use

CRISPR

Tags

HA, His6 (N terminal on backbone), NLS, Nucleopla…

Expression

Bacterial

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pNCS-CyOFP

Plasmid

#74278

Purpose

Constitutive expression of CyOFP in bacteria

Depositor

Michael Lin

Article

Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

CyOFP

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-MBP-TEV site-TadA8.20

Plasmid

#194702

Purpose

Expresses N-terminal MBP fused TadA8.20 in bacteria

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

TadA8.20

Tags

6xHis and MBP

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Promoter

T7 promoter

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(4,5)P2 Probe

Plasmid

#211513

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

PLCdelta1-PH

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCA24N-sso7d-ligase

Plasmid

#87745

Purpose

IPTG-inducible expression of sso7d-ligase fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and codon optimized sso7d (Sulfolobus solfa…

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMVV205

Plasmid

#215342

Purpose

Recombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backbone

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-MBP-TEV site-TadA8r

Plasmid

#208318

Purpose

Overxpression MBP-TadA8r

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-c2X_MBP-CLmN-H6

Plasmid

#234487

Purpose

Maltose binding protein N-terminally fused to His-tagged CLmN split intein

Depositor

Henning Mootz

Article

Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

Insert

N-terminal Fragment of the CLm Split Intein

Tags

Hexahistidine Tag and Maltose Binding Protein (MB…

Expression

Bacterial

Mutation

T69K, F75H, M118N within the N-terminal fragment …

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAB_PalkB_sfgfp_KanR

Plasmid

#166503

Purpose

Expression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.

Depositor

Sven Panke

Article

Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PalkB

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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