30,736 results
-
Plasmid#73236PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAPK8 (MAPK8 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00069:Pkinase 26-321PromoterT7Available SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1_WT RAD23B
Plasmid#201457PurposeExpresses human RAD23B with an N-terminal GST tag in E. coliDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
TagsGSTExpressionBacterialMutationCoding sequence has been optimised for expression…Promotertac promoterAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pExchange_tdk_BT1311p-tetR-1
Plasmid#90325PurposeSuicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702DepositorInsertTetR
ExpressionBacterialAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJJS001
Plasmid#188324PurposePCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::GFP::Linker
ExpressionBacterialAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMV306DIhsp+LuxG13
Plasmid#49999PurposeIntegrase removed to increase stability of the plasmid in mycobacteriaDepositorInsertBacterial luciferase operon + G13 promoter
ExpressionBacterialMutationIt contains a gram-positive enhanced translation …PromoterG13 from M. marinum G13 promoter driving luxCAvailable SinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-HA2-GFP-HiBit
Plasmid#171017PurposeFor bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-HA2-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAA3.stelN
Plasmid#112011PurposeExpresses TelN enzymeDepositorInsertTelN
Tagsstrep and hisExpressionBacterialPromoterT7Available SinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-HaloTag7
Plasmid#167266PurposeOverexpression of HaloTag7 in E. coliDepositorInsertHaloTag7
TagsHis x10ExpressionBacterialPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-4xTR-TUBE
Plasmid#110312PurposeE. coli expression and purification of 4xTR-TUBEDepositorInsert4xTR-TUBE (UBQLN1 Human)
TagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
lox2272 Neo* lox2272
Plasmid#168769Purposelox2272 (lox variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassetteDepositorInsertNeo*
UseCre/Lox and Mouse TargetingExpressionBacterial and MammalianAvailable SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
KG#908
Plasmid#110914PurposeExpresses the plant proteins TIR1 (necessary for Auxin-Inducible Degradation) pan-neuronally in C. elegansDepositorInsertsrab-3 promoter
TIR1 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
ExpressionBacterialAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crE6
Plasmid#172928PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 1 helix and 2 GSG linkersDepositorInsertCrowdingSensorE6
TagsHisExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseB-6xHis
Plasmid#214809PurposeExpression of 6xhis-tagged micrococcal nuclease B (MNaseB)DepositorInsertOmpA-Micrococcal nuclease B
Tags6xhisExpressionBacterialPromoterT7 promoterAvailable SinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHP_YEAST
Plasmid#204233PurposeBacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHP_YEAST (MDH3 Budding Yeast)
Tags6X HisExpressionBacterialMutationThe last 3 C-terminal residues (SKL) are the pero…PromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cys-3HB-SPYcatcher
Plasmid#214838PurposeThis plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-3HB-SPYcatcher
TagsHis-TEV-SUMO-ExpressionBacterialPromoterT5Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher-mi3
Plasmid#112255PurposeExpresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccinationDepositorInsertSpyCatcher-mi3
TagsC-tagExpressionBacterialPromoterT7Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R124A wgMDH
Plasmid#204257PurposeBacterial expression of R124A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR124AAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHJL1
Plasmid#199376PurposeEncodes dual cistronic cytosolic GCaMP6s and mScarlet-I optimized for C. elegans expressionDepositorInsertGCaMP6s::SL2::mScarlet-I
ExpressionBacterialMutationno mutationsAvailable SinceApril 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL97
Plasmid#78576PurposeOver-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoterDepositorTypeEmpty backboneUseStreptomyces expressionExpressionBacterialPromoterermEp* promoter from S. erythraeaAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pnsgRNA
Plasmid#172717PurposeDelivery of guide RNA for prime editingDepositorInsertJ23119-gRNA
ExpressionBacterialAvailable SinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only