32,771 results
-
Plasmid#27118DepositorInsertMS2 binding sites (6)
ExpressionBacterialAvailable SinceDec. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pN251
Plasmid#188471PurposeLentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)DepositorInsertenCas12a-HF (gift from Dr. Keith Joung)
UseLentiviralExpressionBacterial and MammalianAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
TOPO mKate2
Plasmid#68441PurposeTOPO construct expressing mKate2 for generation of GMAP-compatible geneDepositorInsertmKate2
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
VRK1_HUMAN_D0
Plasmid#79684PurposeThis plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_hsHUR-Halo
Plasmid#175303PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_TKT-Halo
Plasmid#175331PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
YopH-pNIC-Bsa4
Plasmid#92063PurposepNIC expression vector encoding gene for YopHDepositorInsertYopH
Tags6x His TagExpressionBacterialMutationCodon optimized E. coliAvailable SinceJan. 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSCARS1
Plasmid#174868PurposeContains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1DepositorInsertARS1
UseSynthetic BiologyExpressionBacterial and YeastAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSP90AA1
Plasmid#177658PurposeRecombinant protein synthesis of human HSP90AA1DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF_6xHIS_Csx29
Plasmid#197613PurposeFor protein purification of Csx29DepositorInsert6-HIS tagged Csx29
ExpressionBacterialAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYGD
Plasmid#80755PurposeExpression of human gamma D-crystallin in E. coliDepositorAvailable SinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCyPet-His
Plasmid#14030DepositorInsertCyPet
Tags6xHisExpressionBacterialAvailable SinceFeb. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα11-LgB97
Plasmid#134361PurposeNanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-WT-LanCL2-C-Ter-His
Plasmid#154186PurposeTo express LanCL2 in bacterial cellsDepositorInsertLanCL2
TagsHexahistidineExpressionBacterialAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 NDM-1
Plasmid#112883PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα13-LgB106
Plasmid#134364PurposeNanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TetOn-dCas9-KRAB
Plasmid#115545PurposeDoxycycline inducible dCas9-KRAB targeting construct for AAVS1 locusDepositorTypeEmpty backboneTagsHAExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-sumo-NSP12 (SARS-CoV-2)
Plasmid#159107PurposeThe SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).DepositorInsertNSP12
TagsHis-SUMO tagExpressionBacterialMutationNonePromoterT7Available SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2782
Plasmid#160668PurposeMobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAsDepositorTypeEmpty backboneExpressionBacterialAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG87 FAST
Plasmid#130719PurposeExpresses His-tagged FAST (also called YFAST) in bacteriaDepositorInsertFAST
TagsHis-tagExpressionBacterialPromoterT7Available SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UnaG
Plasmid#203485PurposeFor recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.DepositorInsertUnaG
ExpressionBacterial and PlantAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
M13-kmR
Plasmid#206861PurposeM13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)DepositorInsertKanamycin resistance gene
ExpressionBacterialPromoterlacAvailable SinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYTK001
Plasmid#65108PurposePart Plasmid Entry Vector to be used in the Dueber YTK system.DepositorInsertnone
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-human APPL1
Plasmid#22198DepositorInsertAdaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
TagsGFPExpressionBacterial and MammalianAvailable SinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDusk
Plasmid#43795PurposeEmpty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBbB6c-GFP
Plasmid#35356DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialPromoterLlacO-1Available SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
KCTD16A
Plasmid#74657PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Pediculus humanus corporis PINK1 (115 - 575)
Plasmid#110750PurposeBacterial expression of PhPINK1 (biochemistry)DepositorInsertPhum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
TagsN-His6-GST-3CExpressionBacterialPromoterT7Available SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM33_PEX14_Pex14
Plasmid#178484PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tn7 integration plasmid
Plasmid#154134PurposeSuicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.DepositorInsertsleft and right end of Tn7 arm
Spectinomycin resistant gene
ExpressionBacterialPromoterSpectinomycin resistance gene promoter and noAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only