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Showing: 3761 - 3800 of 29646 results

  1. pDOC-K-glmS-GFPfor

    Plasmid
    #158059
    Purpose
    Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable marker
    Depositor
    Mark Webber
    Article
    Holden et al Microbiology (Reading). 2020 Nov 23. doi
    Insert
    Green Fluorescent Protein optimised for excitation with UV light
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    acpP
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    158059-plasmid-map-sequence-id-309439

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  4. pMU131

    Plasmid
    #66811
    Purpose
    Shuttle vector with gram-positive origin of replication isolated from a native plasmid of Thermoanaerobacterium saccharolyticum B6A-RI
    Depositor
    Lee Lynd
    Article
    Shaw et al Appl Environ Microbiol. 2010 Jul;76(14):
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    66811-plasmid-map-sequence-id-117172

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  5. INPP5EA

    Plasmid
    #42478
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    INPP5EA (INPP5E Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pTO149_RFP_CauNEO

    Plasmid
    #177277
    Purpose
    Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.
    Depositor
    Teresa O'Meara
    Article
    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:
    Insert
    RFP, CauNEO
    Use
    Tags
    ENO1-RFP
    Expression
    Bacterial
    Mutation
    All CUG Leucine codons were replaced with the syn…
    Promoter
    AgTEF2
    Available since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    177277-plasmid-map-sequence-id-347241

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  11. pDL14

    Plasmid
    #106901
    Purpose
    pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0
    Inserts
    gam
    bet
    mutS
    lacI
    rhaR
    rhaS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Lysine 622 was replaced by Alanine
    Promoter
    Available since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    106901-plasmid-map-sequence-id-334155

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  12. pS3

    Plasmid
    #50596
    Purpose
    Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5
    Depositor
    Jay Keasling
    Article
    Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8
    Inserts
    aroE
    aroD
    aroB
    aroG*
    ppsA
    tktA
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lacUV5
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    50596-plasmid-map-sequence-id-77162

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  13. pYTK089

    Plasmid
    #65196
    Purpose
    Encodes AmpR-ColE1 as a Type 8a part to be used in the Dueber YTK system
    Depositor
    John Dueber
    Article
    Lee et al ACS Synth Biol. 2015 Apr 14.
    Insert
    AmpR-ColE1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    65196-plasmid-map-sequence-id-110249

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  18. AWP-031

    Plasmid
    #213967
    Purpose
    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.
    Depositor
    Alexander Westermann
    Article
    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121
    Insert
    Cas12a (cas12a Synthetic, Segatella bryantii)
    Use
    Tags
    3XFLAG
    Expression
    Bacterial
    Mutation
    D875A to inactivate target nuclease activity
    Promoter
    PcfxA, IPTG inducible
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213967-plasmid-map-sequence-id-429166

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  19. CAMK2D

    Plasmid
    #38904
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CAMK2D (CAMK2D Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. ASSA

    Plasmid
    #42368
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ASSA (ASS1 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  22. pPGN-C

    Plasmid
    #174578
    Purpose
    pOPIN-GG expression vector compatible with N-terminal tagging, carbenicillin resistant
    Depositor
    Mark Banfield
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174578-plasmid-map-sequence-id-344737

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  23. pCT02

    Plasmid
    #212152
    Purpose
    Plasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.
    Depositor
    Kang Zhou
    Insert
    FAST-PETase
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    S121E, D186H, R224Q, N233K, R280A
    Promoter
    PT7
    Available since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    212152-plasmid-map-sequence-id-424774

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  24. 3xFLAG-dCas9/p-bacteria

    Plasmid
    #64325
    Purpose
    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do
    Insert
    3xFLAG-dCas9
    Use
    CRISPR; Tetracycline-inducible
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pLtetO-1
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    64325-plasmid-map-sequence-id-107969

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  38. pET14b-dabAB2

    Plasmid
    #133013
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Use
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    t7
    Available since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    133013-plasmid-map-sequence-id-262987

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  39. pET-HIS-Sangamo

    Plasmid
    #40786
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    N Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40786-plasmid-map-sequence-id-68901

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  40. RARS_pNIC-Bio3

    Plasmid
    #153055
    Purpose
    Expression construct for producing Avi tagged Arginyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    RARS (RARS1 Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153055-plasmid-map-sequence-id-304475

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Showing: 3761 - 3800 of 29646 results