33,434 results

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pSG 25

Plasmid

#63867

Purpose

Expression of beta-galactosidase at high levels in E. coli

Depositor

Albert Dahlberg

Publication

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Promoter

PtacI

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
mPapaya-pBAD

Plasmid

#54838

Purpose

Localization: Protein Expression Vector , Excitation: 530, Emission: 541

Depositor

Robert Campbell, Michael Davidson

Publication

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Type

Empty backbone

Tags

6xHis and mPapaya

Expression

Bacterial

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBRα

Plasmid

#53731

Purpose

Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

Depositor

Ann Hochschild

Publication

Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Insert

αNTD

Expression

Bacterial

Promoter

lpp/lacUV5 (tandem promoter)

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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JF001A

Plasmid

#53116

Purpose

expresses alpha hemolysin under the control of theophylline riboswitch

Depositor

Sheref Mansy

Publication

Lentini et al Nat Commun. 2014 May 30;5:4012. doi: 10.

Insert

theophylline riboswitch and alpha hemolysin

Expression

Bacterial

Promoter

T7 promoter

Available since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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Split 'n coexpress CUL3-RBX1

Plasmid

#52293

Purpose

bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1

Depositor

Brenda Schulman

Publication

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Inserts

Cul3 N-terminal domain (CUL3 Human)
Cul3 C-terminal domain (CUL3 Human)
RBX1 (Rbx1 Mouse)

Tags

GST, IRES, and TEV

Expression

Bacterial

Mutation

contains aa 1-309, contains aa 310-768, and conta…

Promoter

IRES and tac

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Nano-lantern/pRSETB

Plasmid

#51969

Purpose

bacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Publication

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pJS167

Plasmid

#48881

Purpose

Positive feedback plasmid from oscillator

Depositor

Jeff Hasty

Publication

Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

Inserts

araC gene with ssrA tag
monomeric yeast-enhanced green fluorescent protein

Use

Synthetic Biology

Tags

ssrA (TSAANDENYALAA)

Expression

Bacterial

Promoter

Para/lac

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pJS169

Plasmid

#48882

Purpose

Negative feedback plasmid from oscillator

Depositor

Jeff Hasty

Publication

Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

Insert

lacI with ssrA tag

Use

Synthetic Biology

Tags

ssrA (TSAANDENYALAA)

Expression

Bacterial

Promoter

Para/lac

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-ATG8(414)/GFP-AUT7(414)

Plasmid

#49424

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.

Depositor

Daniel Klionsky

Publication

Abeliovich et al Mol Biol Cell. 2003 Feb;14(2):477-90.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

ATG8

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
p44K

Plasmid

#45800

Depositor

Luc Ponchon

Publication

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRAD1

Plasmid

#47697

Purpose

General cloning vector for use in E. coli or D. radiodurans

Depositor

Mary Lidstrom

Publication

Meima et al Appl Environ Microbiol. 2000 Sep;66(9):3

Type

Empty backbone

Expression

Bacterial

Promoter

repU

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pOSIP-CT (CamR, P21)

Plasmid

#45981

Depositor

Drew Endy, Keith Shearwin

Publication

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGEX Grb2-SH2

Plasmid

#46440

Depositor

Bruce Mayer

Publication

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb2 (GRB2 Human)

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 58-159

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMAL-XPG

Plasmid

#39215

Depositor

Aziz Sancar

Publication

Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

Insert

XPG (ERCC5 Human)

Tags

MBP

Expression

Bacterial

Mutation

Note that XPG starts at amino acid 5

Promoter

Ptac

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pKD-SepRS-EFSep

Plasmid

#34623

Depositor

Jesse Rinehart, Dieter Söll

Publication

Park et al Science. 2011 Aug 26;333(6046):1151-4.

Inserts

SepRS (MMP0688 Methanocaldococcuc maripaludis)
EF-Sep (tufB E. coli)

Expression

Bacterial

Mutation

His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

Promoter

Ptac and Ptac (from SepRS- operon like)

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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His-PIMT

Plasmid

#34852

Depositor

Bruce Downie

Publication

Dinkins et al Plant J. 2008 Jul;55(1):1-13.

Insert

PIMT (PCMT1 Human)

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
DCX-RFP

Plasmid

#32851

Depositor

Joseph Gleeson

Publication

Tanaka et al J Cell Biol. 2004 Jun 7. 165(5):709-21.

Insert

DCX (DCX Human)

Tags

DsRed2

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pET mCerulean LIC cloning vector (u-mCerulean)

Plasmid

#29773

Depositor

Scott Gradia

Type

Empty backbone

Tags

mCerulean

Expression

Bacterial

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pET-3c-rhVTN-N

Plasmid

#30225

Depositor

James Thomson

Publication

Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

Insert

rhVitronectin with N terminus cleavage (VTN Human)

Expression

Bacterial

Mutation

deleted amino acids: 20*-62

Available since

May 25, 2011

Availability

Academic Institutions and Nonprofits only

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pET28a-ec_bPAC

Plasmid

#28135

Purpose

Expression of photoactivated cyclase bPAC of Beggiatoa sp. optimized for E. coli

Depositor

Peter Hegemann

Publication

Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

Insert

E.coli adapted photoactivated adenylyl cyclase

Tags

His

Expression

Bacterial

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only

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