Addgene

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PB-EF1a-TetOn3G

Plasmid

#104543

Purpose

PiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoter

Depositor

David Vereide

Article

Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

Insert

Tet-On 3G

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGP-RSET-iGABASnFR2-6xHis

Plasmid

#218885

Purpose

Bacterial expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2

Use

Tags

6xHis

Expression

Bacterial

Mutation

S99A F102Y F104Y L178S

Promoter

T7

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD/myc-his A Rv0712 (FGE)

Plasmid

#16132

Purpose

Bacterial expression of Mycobacterium tuberculosis FGE

Depositor

Carolyn Bertozzi

Article

Carrico et al Nat Chem Biol. 2007 Jun . 3(6):321-2.

Insert

Formylglycine-generating enzyme

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2007

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA

Plasmid

#81185

Purpose

Expresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5c

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Inserts

Cas5c
Cas8c
Cas7

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_MBP-nLucCP-67/68

Plasmid

#113922

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-67/68

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pL0-BvDODAα1

Plasmid

#162528

Purpose

Beta vulgaris 4,5-DOPA dioxygenase alpha 1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

DODAα1

Use

Puc19-derived

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a-C2-mKate

Plasmid

#208763

Purpose

To express C2-mKate in bacteria

Depositor

Urte Neniskyte

Article

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2-mKate (Mfge8 Mouse)

Use

Tags

mKate

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn A53T

Plasmid

#105727

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

Insert

alpha-synuclein A53T (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

A53T

Promoter

T7

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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p324_DG

Plasmid

#234690

Purpose

Golden gate domesticated pET28b with TEV-His6

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

TEV-His6

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p370_DG

Plasmid

#234693

Purpose

Golden gate domesticated pET28b with 3C-mNeongreen-His10

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

3C-mNeongreen-His10

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p277_DG

Plasmid

#234695

Purpose

Golden gate domesticated pET28b for scarless cloning with His6-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His6-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p357_DG

Plasmid

#234700

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-GST-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-GST-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p368_DG

Plasmid

#234691

Purpose

Golden gate domesticated pET28b with 3C-His6

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

3C-His6

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p349_DG

Plasmid

#234679

Purpose

Golden gate domesticated pET28b with His10-GST-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-GST-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p351_DG

Plasmid

#234681

Purpose

Golden gate domesticated pET28b with His10-mNeongreen-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-mNeongreen-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p364_DG

Plasmid

#234686

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8 and Opsin

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-bdNEDD8 and Opsin

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p369_DG

Plasmid

#234692

Purpose

Golden gate domesticated pET28b with 3C-His10

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

3C-His10

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p344_DG

Plasmid

#234674

Purpose

Golden gate domesticated pET28b with His6-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His6-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p367_DG

Plasmid

#234689

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8 and 2X Strep tag

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

2X Strep tag and His10-bdNEDD8

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p354_DG

Plasmid

#234697

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-TEV

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge