We narrowed to 151 results for: T7 rna polymerase

1. pCS4333 yeTadA1.0-T7 RNAP

   Plasmid

   #137735

   Purpose

   pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

   Depositor

   Christina Smolke

   Article

   Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

   Insert

   yeTadA1.0-T7 RNAP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   E366G (See depositor comment below)

   Promoter

   Available since

   Jan. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p6XHis-T7(P266L)

   Plasmid

   #174866

   Purpose

   Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

   Depositor

   Anna Pyle

   Article

   Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

   Insert

   T7 RNA polymerase
   

   Use

   Tags

   6XHis-tag

   Expression

   Bacterial

   Mutation

   P266L

   Promoter

   lac promoter

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBP-T7RNAP

   Plasmid

   #74096

   Purpose

   T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   T7 RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p70a-T7RNAP

   Plasmid

   #105683

   Purpose

   Expresses T7 polymerase in cell-free TXTL.

   Depositor

   Chase Beisel

   Article

   Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

   Insert

   T7 polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSC101-pT7-T3RNAP

   Plasmid

   #140872

   Purpose

   T7 promoter driven synthesis of T3 RNA polymerase

   Depositor

   Neal Devaraj

   Article

   Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

   Insert

   T3 RNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. T7RNAP Cyslight

   Plasmid

   #205077

   Purpose

   T7RNAP protein expression

   Depositor

   Smita Patel

   Article

   Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

   Insert

   T7RNAP Cyslight (T7p07 Bacteriophage T7)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C125S, C347S, C510S, C515S, C530S, C723S

   Promoter

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. T7RNAP Cyslight V174C

   Plasmid

   #205078

   Purpose

   T7RNAP protein expression

   Depositor

   Smita Patel

   Article

   Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

   Insert

   T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C125S, C347S, C510S, C515S, C530S, C723S V174C

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT7RNAPNev-NB1

   Plasmid

   #216336

   Purpose

   Arabinose-inducible PBad promoter driving expression of the evolved N-terminal portion (residues 1-179) of T7RNAP translationally fused to anti-eGFP nanobody NB1

   Depositor

   Mark Styczynski

   Article

   McSweeney et al Sci Adv. 2025 Feb 21;11(8):eado6280. doi

   Insert

   T7RNAPN-NB1 fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 3, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pQE-WT-RGVG-M6

   Plasmid

   #70172

   Purpose

   Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

   Depositor

   Andrew Ellington

   Article

   Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

   Insert

   RGVG-M6 T7 RNA polymerase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T5/Lac

   Available since

   Dec. 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pEcRNAP6

    Plasmid

    #128940

    Purpose

    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z

    Depositor

    Seth Darst

    Article

    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

    Inserts

    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    PreScission protease site (GE Healthcare; LEVLFQG…

    Promoter

    T7

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEcRNAP5

    Plasmid

    #128939

    Purpose

    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, Z

    Depositor

    Seth Darst

    Article

    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

    Inserts

    RpoAppx234-241-(His)10 (rpoA Escherichia coli)
    RpoB
    RpoC
    RpoZ
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Residues 234-241 are substituted for a PreScissio…

    Promoter

    T7

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE30-His-T7RNAP

    Plasmid

    #124138

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    T7 RNA polymerase (T7p07 Enterobacteria phage T7)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7RNAP

    Plasmid

    #170101

    Purpose

    expresses T7 RNAP

    Depositor

    Chase Beisel

    Article

    Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

    Insert

    T7-RNA polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTara:500

    Plasmid

    #60717

    Purpose

    Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    T7 RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTARA

    Plasmid

    #31491

    Depositor

    Kathleen Matthews

    Article

    Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

    Insert

    RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N823D

    Promoter

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTHSSd_18

    Plasmid

    #59953

    Purpose

    Quad split (at 61, 179, 601-SZ)

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    Four-fragment T7* RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PTac

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTHSSd_14

    Plasmid

    #59952

    Purpose

    Triple split (at 67, 601-SZ)

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    Three-fragment T7* RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PTac

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAIP4_5EF9

    Plasmid

    #234154

    Purpose

    Heterologous protein expression of polymerase basic protein 2 (RNA-directed RNA polymerase subunit P3) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5EF9 (PB2 Influenza B virus (B/Lee/1940))
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGEMABC

    Plasmid

    #45398

    Depositor

    Katsu Murakami

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Inserts

    rpoA (rpoA Escherichia coli)
    rpoB (rpoB Escherichia coli)
    rpoC (rpoC Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None and T7

    Available since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQE 80L Kan T7WT

    Plasmid

    #102790

    Purpose

    Expresses T7 RNA polymerase

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    T7 RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge