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Showing: 301 - 330 of 4828 results

  5. pTriEx-mCherry-PA-Rac1-C450A

    Plasmid
    #22028
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
    Use
    Tags
    6xHis and mCherry
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    22028-plasmid-map-sequence-id-213358

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  6. pUASp YFP Ras85D CA

    Plasmid
    #52247
    Purpose
    Drosophila expression of YFP-Ras85D constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Ras85D (Ras85D Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q61L; CA mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid
    #115694
    Purpose
    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer
    Article
    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:
    Inserts
    NmeCas9
    Nme-sgRNA
    Use
    CRISPR
    Tags
    NLS and NLS, HA
    Expression
    Insect and Mammalian
    Mutation
    None and fusion of repeat/tracrRNA to make a sgRNA
    Promoter
    EF-1 alpha and U6
    Available since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    115694-plasmid-map-sequence-id-221986

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  15. pUASTLOTattB_VHH-GFP4::CD8::mCherry

    Plasmid
    #163917
    Purpose
    Expression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancers
    Depositor
    Markus Affolter
    Article
    Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do
    Insert
    Fusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163917-plasmid-map-sequence-id-323453

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  20. pTriEx-PA-Rac1

    Plasmid
    #22024
    Purpose
    Expression of photoactivatable Rac1
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    Use
    Tags
    6X His
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only

  23. pBac[3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40]

    Plasmid
    #86453
    Purpose
    UAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Transformation plasmid; Red eye marker)
    Depositor
    Gregor Bucher
    Insert
    3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86453-plasmid-map-sequence-id-171157

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  27. pDest-795

    Plasmid
    #161881
    Purpose
    Standard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions using the Bac-to-Bac insect cell system
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Tags
    MBP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    161881-plasmid-map-sequence-id-322112

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  30. pFastBacHTa_HCK

    Plasmid
    #224245
    Purpose
    Expression of human HCK Kinase in insect cells (SF9 cells)
    Depositor
    Andrew Mesecar
    Insert
    hck (HCK Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224245-plasmid-map-sequence-id-458377

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Showing: 301 - 330 of 4828 results