5,046 results
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Plasmid#49401PurposeThe pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.DepositorTypeEmpty backboneUseTALENExpressionInsectAvailable SinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only
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pCasper4-tubulin-IVS-Syn21-QSco-p10
Plasmid#46132DepositorInsertQSco
ExpressionInsectAvailable SinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR4-tubulin-QF#7
Plasmid#46127DepositorInsertQF#7
ExpressionInsectAvailable SinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC-QSco
Plasmid#46106DepositorInsertQSco
ExpressionInsectAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_wt_Venus_wt
Plasmid#39813DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pS3aG
Plasmid#31171PurposeA Drosophila GFP reporter transgene vector for site-specific integrationDepositorTypeEmpty backboneExpressionInsectAvailable SinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMT-Pav-GFP
Plasmid#24286DepositorAvailable SinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDrBB2
Plasmid#18946DepositorInserthygromycin gene driven by the copia promoter, phiC31 attB
ExpressionInsectAvailable SinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 PIK3R1-Ni
Plasmid#17744DepositorAvailable SinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAc5 dOrai
Plasmid#16368DepositorAvailable SinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDW446
Plasmid#8844DepositorTypeEmpty backboneTagsbiotin acceptor peptideExpressionInsectAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSSRB069_pAC8-hsDDB1dB
Plasmid#218790PurposeInsect cell expression vector for 6xHis-TEV-hsDDB1∆BDepositorInsertDNA damage-binding protein 1 (DDB1 Human)
Tags6xHis-TEVExpressionInsectMutationDeleted amino acids 396-705 and replaced with GNG…PromoterpolyhedrinAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWALIUM10-V5-TurboID-GW
Plasmid#196285PurposeGateway destination vector to express N-terminal TurboID fusion under UAS promoter, for making transgenic fliesDepositorTypeEmpty backboneUseGateway CloningExpressionInsectAvailable SinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIEx4-Cre
Plasmid#198280PurposeExpresses Cre in insect cellsDepositorInsertpIEx4-Cre
ExpressionInsectAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Activin A (S2-2)
Plasmid#184621Purposefor transfection into S2 cells and expression of Activin A proteinDepositorAvailable SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUb PspCas13b + PspCas13b guide RNA
Plasmid#176305PurposeExpresses PspCas13b and its associated guide RNA in Drosophila cellsDepositorInsertPspCas13b
TagsHA tagExpressionInsectMutationE113K, N265S- please depositor commentsPromoterUbi-p63eAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAaePUb::Cas9-2A-Neo
Plasmid#176680PurposeExpression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)DepositorInsertspCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
UseCrisprExpressionInsectMutationDrosophila codon optimizedPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7
Plasmid#170141PurposeExpresses CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
Tags6xHis and FLAGExpressionInsectAvailable SinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-C-3xVHH05-DsRed
Plasmid#171580PurposeFor scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3xVHH05 (UBE2J1 Human)
ExpressionInsectAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-Nb127D01-mCherry
Plasmid#171573PurposeExpresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cellsDepositorAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-NbVHH05-GFP
Plasmid#171570PurposeExpresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cellsDepositorAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His10FLAG
Plasmid#157736Purposeempty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneTagsHis10 + 3xFLAG, enterokinase-cleavableExpressionInsectPromoterpolyhedrinAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10
Plasmid#162383PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8s
Tags6xHisExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-3xFLAG-hSpCas9
Plasmid#162161PurposeExpresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)DepositorInsertshSpCas9
sgRNA
UseCRISPRTags3xFLAGExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9
Plasmid#162162PurposeExpresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)DepositorInsertshSpCas9
sgRNA
UseCRISPRTagsN/AExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.AEBP2
Plasmid#125165PurposeExpresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVL1393-6xMyc
Plasmid#128035PurposeBaculovirus expression vector with C-terminal 6xMyc tagDepositorTypeEmpty backboneTags6xMyc tagExpressionInsectAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293
Plasmid#118412PurposeExpresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.DepositorInsertFirefly luciferase
UseAAV, Luciferase, and Synthetic BiologyExpressionInsect and MammalianPromoterEF1aAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_His-ZZ-TEV-Lis1
Plasmid#132539PurposePlasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tagDepositorAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only