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Plasmid#49327PurposeDonor plasmid for homologous recombination that expresses dsRED constitutively using the Aedes aegypti polyubiquitin (PUb) promoter.DepositorInsertdsRED
UseTagsExpressionInsectMutationPromoterAedes polyubiquitinAvailable sinceNov. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-wt
Plasmid#53095PurposeExpresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.DepositorInsertLOV(wt)-6aa-DAD
UseTags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in VenusPromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBacI-KDAC8 H143A
Plasmid#224344PurposeExpresses human KDAC8 (HDAC8) H143A in insect cellsDepositorInsertKDAC8 (HDAC8 Human)
UseTagsTEV-cleavable His6ExpressionInsectMutationHistidine 143 mutated to alaninePromoterPolyhedrinAvailable sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBacI-KDAC8 D101E
Plasmid#224350PurposeExpresses human KDAC8 (HDAC8) D101E in insect cellsDepositorInsertKDAC8 (HDAC8 Human)
UseTagsTEV-cleavable His6ExpressionInsectMutationAspartate 101 mutated to glutamatePromoterPolyhedrinAvailable sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUASTattB-2xFlag-mCherry-Msp300KASH
Plasmid#170807PurposeExpresses Msp300 KASH domain fused to 2xFLAG and mCherry under Gal4/UAS control for Drosophila transgenesisDepositorInsert2xFLAG/mCherry-Msp300KASH
UseTags2xFLAG-mCherry and Msp300KASHExpressionInsectMutationPromoter5xUAS + hsp70 minimal promoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC7
Plasmid#118876Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_N116C-G193C
Plasmid#162580PurposeExpresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationAsparagine 116 was mutated to Cysteine and Glycin…PromoterpolyhedrinAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-frame_selector_0
Plasmid#127553PurposeDrosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 0
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
R979-X58-008: Hs.KRAS4b(1-188)
Plasmid#159691PurposeBaculovirus protein expression of KRAS4b (1-188)DepositorInsertHs.KRAS4b(1-188) (KRAS Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFRC14-10XUAS-IVS-GFP-WPRE
Plasmid#26223DepositorInsertGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pCFD7
Plasmid#73916PurposegRNA vector for AsCpf1DepositorInserttRNA:hAsCpf1-gRNA:tRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR R4-vas2-integrase-R3
Plasmid#62299PurposephageC31 integrase-expressing helper plasmid for Anopheles transgenesis, vasa promoterDepositorInsertphageC31 integrase under control of vasa promoter and terminator
UseTagsHA and SV40 NLSExpressionInsectMutationPromotervasaAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFBD-ESCRT 0
Plasmid#21499DepositorInsertpFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Mouse, Human)
UseTagsGST and His-MBPExpressionInsectMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pE2n
Plasmid#17456Purposemodified pENTR vector for N-terminal 3x HA tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags3xHAExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUAST-attB-3xHA-V5-RpL10Ab
Plasmid#125223Purpose3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expressionDepositorInsertRpL10Ab (RpL10Ab Fly)
UseTags3x HA, V5ExpressionInsectMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with PA+Histag
Plasmid#84870PurposeBaculovirus Expression of Bacillus anthracis protective antigenDepositorInsertPA
UseBaculovirusTagsHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFBNb-cALFA
Plasmid#159990PurposeFor the protein expression with ALFA tag at C-terminus in insect cellsDepositorTypeEmpty backboneUseTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionInsectMutationPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT Draper-Ala11-GFP
Plasmid#126654PurposeInducible expression of Draper-Ala11-GFP mutant in S2 cellsDepositorInsertDraper cytoplasmi Tyr residues mutated to Ala
UseTagsExpressionInsectMutationCytoplasmic Tyr residues to AlaPromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT Shark-TagBFP
Plasmid#126658PurposeInducible expression of Shark-TagBFP in S2 cellsDepositorInsertShark
UseTagsExpressionInsectMutationPromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - CA
Plasmid#65424PurposeRhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63LDepositorInsertRBD, mCerulean, mVenus, RhoC
UseTagsExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, Q63L in RhoCPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - DN
Plasmid#65423PurposeRhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19NDepositorInsertRBD, mCerulean, mVenus, RhoC
UseTagsExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, T19N in RhoCPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pFB1.HMBP.PrS.SUZ12
Plasmid#125162PurposeExpresses human SUZ12 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertPolycomb protein SUZ12 (SUZ12 Human)
UseBaculovirus expression vectorTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
UAS-u(S)Cas9
Plasmid#127383PurposeExpresses Cas9 at high levelDepositorInsertuORF(S)-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterUASTAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pAcGHLT-B-DDB1
Plasmid#48638PurposeExpresses Full Lenght DDB1 in Insect Cells (ampR)DepositorInsertDDB1 (DDB1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
Orco-T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed
Plasmid#157974PurposeTemplate plasmid for inserting GCaMP reporter into the Orco gene of Aedes aegypti with CRISPR/Cas9DepositorInsertOrco
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-C-3x127D01-DsRed
Plasmid#171578PurposeFor scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3x127D01 (CXCR2 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pE5c
Plasmid#17465Purposemodified pENTR vector for C-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only