5,013 results

Showing: 631 - 660 of 5013 results

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pBFv-U6.2B

Plasmid

#138401

Purpose

gRNA expression vector; Can be combined with pBFv-U6.2 to generate double-gRNA expression vector

Depositor

Shu Kondo

Article

Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

Insert

U6-2

Expression

Insect

Promoter

U6-2

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pHACK-GAL4>QF2(newHA1)

Plasmid

#104873

Purpose

HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

T2A-QF2

Use

CRISPR

Expression

Insect

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pattB-synaptobrevin-7-QFBDAD-hsp70

Plasmid

#46115

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7

Expression

Insect

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pBac-DsRed-ORCO_9kbProm-QF2

Plasmid

#104877

Purpose

Anopheles gambiae ORCO enhancer/promter to drive QF2 expression in Anopheles transgenic mosquitoes

Depositor

Christopher Potter

Article

Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

Insert

AgORCO-QF2

Expression

Insect

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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p{CFD4-EYFP-3xP3::DsRed}

Plasmid

#86863

Purpose

Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Type

Empty backbone

Expression

Insect

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCFD5-U6-3-t-attP40

Plasmid

#133561

Purpose

gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

attP40 region guide RNAs

Use

CRISPR

Expression

Insect

Promoter

U6

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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ovoD1 D1B2NR (18 kb)

Plasmid

#37280

Depositor

Norbert Perrimon

Article

Chou et al Development. 1993 Dec;119(4):1359-69.

Insert

ovoD1 genomic DNA fragment

Expression

Insect

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
Brp-T2A-QF2w-Hsp70-3XP3-dsRed

Plasmid

#141094

Purpose

Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

Depositor

Carolyn McBride

Article

Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

Insert

bruchpilot

Use

CRISPR

Expression

Insect

Mutation

coding sequence downstream of CRISPR cut site, sy…

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.RBBP4

Plasmid

#125164

Purpose

Expresses human RBBP4 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Histone-binding protein RBBP4 (RBBP4 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBFv-nosP-Cas9

Plasmid

#138402

Purpose

Expresses Cas9 in the germline of Drosophila

Depositor

Shu Kondo

Article

Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

Insert

Cas9-NLS

Expression

Insect

Promoter

UAS

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pJFRC8-40XUAS-IVS-mCD8::GFP

Plasmid

#26221

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

mCD8::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_VHH-GFP4::CD8::mCherry

Plasmid

#163917

Purpose

Expression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancers

Depositor

Markus Affolter

Article

Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

Insert

Fusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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891 pFastBac GST PTEN

Plasmid

#10937

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Insect

Available Since

Jan. 3, 2006

Availability

Academic Institutions and Nonprofits only
pDR125

Plasmid

#37150

Depositor

Dale Ramsden

Insert

lig4 (LIG4 Human)

Tags

H6

Expression

Insect

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
pB[U6-3 wex2 PUb-DsRed]

Plasmid

#169010

Purpose

For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

white ex2 sgRNA

Use

Piggybac

Expression

Insect

Promoter

Drosophila melanogaster U6:3

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pQUASp-mCD8mCherry

Plasmid

#46164

Purpose

Expression of murine CD8mCherry in insects

Depositor

Christopher Potter

Insert

mCD8mCherry

Expression

Insect

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pFL His-p97

Plasmid

#113510

Purpose

Expression of His-p97 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97 (VCP Human)

Tags

6*His

Expression

Insect

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCoofy11

Plasmid

#44002

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His10

Expression

Insect

Promoter

hr5

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCaSpeR4-tubulin-QF#7

Plasmid

#46127

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7

Expression

Insect

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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20XUAS IVS ChrimsonR mCherry_tr

Plasmid

#111546

Purpose

Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR_mCherry_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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p1177-X1-8

Plasmid

#38150

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2011 May;77(1):53-61

Insert

Carboxypeptidase A

Tags

HHHHHHRR

Expression

Insect

Promoter

polyhedron

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Flag-Timeless

Plasmid

#23119

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Timeless (TIMELESS Human)

Tags

Flag

Expression

Insect

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pF3BGX-T2A-p65-AD

Plasmid

#138395

Purpose

RMCE vector for generating C-terminal T2A-p65-AD fusion (split GAL4)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-p65-AD

Expression

Insect

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pTS26_QUAS_CsChrimson

Plasmid

#175548

Purpose

Expresses the optogenetic channel CsChrimson off of a QUAS promoter and contains Piggybac terminal repeats for transposon-mediated integration

Depositor

Leslie Vosshall

Article

Sorrells et al Elife. 2022 May 12;11:e76663. doi: 10.75

Insert

CsChrimson

Tags

tdTomato

Expression

Insect

Promoter

QUAS

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAcRP67A prM/E YFV

Plasmid

#52883

Purpose

expression of Yellow Fever Virus VLPs in baculovirus

Depositor

Yorgo Modis

Insert

prM/E

Expression

Insect

Promoter

Polyhedrin

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
pUAST-attB-GFP-V5-His

Plasmid

#85621

Purpose

Expresses GFP and V5 in Drosophila.

Depositor

Kenneth Irvine

Article

Rauskolb et al PLoS Biol. 2011 Jun;9(6):e1000624. doi:

Insert

GFP

Tags

V5, His

Expression

Insect

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBac[3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR]

Plasmid

#86449

Purpose

pBAc transposon with transformation marker for Tribolium transgenesis; Drives EGFP expression in the eyes; contains Gal4_ driven by Tribolium heatshock promoter; (Transformation plasmid)

Depositor

Gregor Bucher

Insert

3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR

Expression

Insect

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pRL-ubi63E

Plasmid

#74280

Purpose

Renilla control plasmid driven by the ubiquitin 63E promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Insert

Renilla luciferase

Expression

Insect

Promoter

ubiquitin 63E promoter

Available Since

April 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJC-20xUAS-TALE3-VP64-P10

Plasmid

#104607

Purpose

Expresses TALE3 under UAS control

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

TALE3

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc

Plasmid

#63167

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-cMyc"

Tags

N-myristoylation

Expression

Insect

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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