4,932 results

1. pUAST-attB-3xHA-V5-RpL10Ab

   Plasmid

   #125223

   Purpose

   3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

   Depositor

   Herman Dierick

   Article

   Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

   Insert

   RpL10Ab (RpL10Ab Fly)
   

   Tags

   3x HA, V5

   Expression

   Insect

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIE-TGFB2-H6

   Plasmid

   #128500

   Purpose

   Expresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   TGFB2 (TGFB2 Human)
   

   Tags

   His tag (6x)

   Expression

   Insect

   Promoter

   IE1

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6c-T

   Plasmid

   #117211

   Purpose

   Expresses gRNA in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

   Insert

   u6c promoter-gRNA
   

   Use

   Synthetic Biology

   Expression

   Insect

   Promoter

   U6

   Available Since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOCC304

   Plasmid

   #118897

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with TEVprotease and TwinStrep-MB…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT-mCherry-Patronin

   Plasmid

   #31753

   Depositor

   Ron Vale

   Article

   Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

   Insert

   Patronin (Patronin Fly)
   

   Tags

   mCherry

   Expression

   Insect

   Promoter

   Metallothionein

   Available Since

   Sept. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pE4n

   Plasmid

   #17458

   Purpose

   modified pENTR vector for N-terminal GST tag fusion with your gene of interest

   Depositor

   Giovanna Benvenuto

   Article

   Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

   Type

   Empty backbone

   Use

   Modified gateway entry vector

   Tags

   GST and Thrombin site

   Expression

   Bacterial, Insect, Mammalia…

   Available Since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. TH1263-018_Jie_SFPQ_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP

   Plasmid

   #221895

   Purpose

   Shuttle vector for baculovirus production, using FlashBac bacmid

   Depositor

   Anthony Hyman

   Article

   Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

   Insert

   SFPQ (SFPQ Human)
   

   Tags

   6xHis-MBP-PreScission and TEV-mGFP

   Expression

   Insect

   Mutation

   wt

   Promoter

   PH promoter

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac1/MBP-His

   Plasmid

   #38008

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   MBP (Maltose binding protein)
   

   Use

   Baculovirus expression

   Tags

   6xHIS

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pMT mCherry-CaaX

   Plasmid

   #126669

   Purpose

   Inducible expression of mCherry-CaaX in S2 cells

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   mCherry-CaaX
   

   Expression

   Insect

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pScarlessHD-N-3xVHH05-DsRed

    Plasmid

    #171581

    Purpose

    For scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3xVHH05 (UBE2J1 Human)
    

    Expression

    Insect

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFBOH-MHL.SUZ12

    Plasmid

    #125172

    Purpose

    Expresses human SUZ12 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    SUZ12 (SUZ12 Human)
    

    Tags

    His-TEV

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pActPL-Gal4AD

    Plasmid

    #15303

    Depositor

    Benjamin White

    Article

    Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

    Insert

    Gal4AD-ZIP+ (GAL4 )
    

    Expression

    Insect

    Available Since

    July 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pattB-hsp70P-QF#7-hsp70T

    Plasmid

    #46134

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7
    

    Expression

    Insect

    Available Since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pnos-Fok1:dCas9-nos

    Plasmid

    #62210

    Purpose

    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Insert

    Fok1-dCas9
    

    Expression

    Insect

    Promoter

    nanos

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAEL006511-Cas9

    Plasmid

    #100580

    Purpose

    The promoter of AAEL006511 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL006511-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Promoter

    AAEL006511 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDCC5

    Plasmid

    #59984

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Mutation

    D10A

    Promoter

    hsp70Bb

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac1-Ac-AChBP

    Plasmid

    #63128

    Purpose

    Expression of Aplysia californica AChBP

    Depositor

    Titia Sixma

    Article

    Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

    Insert

    AChBP
    

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

18. TH0901-pOCC177-FUS-wt_opt(Nhe)

    Plasmid

    #221885

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP-PreScission and TEV-SNAP-tag

    Expression

    Insect

    Mutation

    wt

    Promoter

    PH promoter

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

    Plasmid

    #55216

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    SNAPf and TwinStrepII

    Expression

    Insect

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. OA-1043

    Plasmid

    #164586

    Purpose

    clone in U6 and DR

    Depositor

    Omar Akbari

    Article

    Sun et al J Vis Exp. 2021 Feb 5;(168). doi: 10.379

    Insert

    U6 and DR
    

    Expression

    Insect

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFB-HTB-ASK1

    Plasmid

    #29517

    Depositor

    Ning Zheng

    Article

    Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

    Insert

    ASK1 (SKP1 Mustard Weed)
    

    Expression

    Insect

    Available Since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

22. VanGlow-RR-DSCP

    Plasmid

    #83339

    Purpose

    for Gateway cloning of enhancer elements upstream of the DSCP and mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

    Depositor

    Cheng-Yu Lee

    Article

    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    mCherry::Renilla(nls)

    Expression

    Insect

    Promoter

    Drosophila Synthetic Core Promoter

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pQUAST-frt-stop-frt-p65AD::CaM

    Plasmid

    #64716

    Purpose

    generating transgenic TRIC flies

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Inserts

    p65AD::CaM
    frt-stop-frt
    

    Expression

    Insect

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUAS-ERK-YFP

    Plasmid

    #42076

    Depositor

    Norbert Perrimon

    Article

    Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

    Insert

    Drosophila ERK ortholog (rl Fly)
    

    Tags

    YFP

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

25. IMPT-6213

    Plasmid

    #99333

    Purpose

    AT2R N terminal BRIL, linker GSGS, delta aa 1-34, delta aa 336-363

    Depositor

    Raymond Stevens

    Article

    Zhang et al Nature. 2017 Apr 20;544(7650):327-332. d

    Insert

    AGTR2 (AGTR2 Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pACEBac1-gamma-TuSC

    Plasmid

    #178079

    Purpose

    Insect cell expression of gamma-TuSC

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Inserts

    gamma-tubulin (TUBG1 Human)
    GCP2 (TUBGCP2 Human)
    GCP3 (TUBGCP3 Human)
    

    Tags

    TEV-His6

    Expression

    Insect

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPMW-attB

    Plasmid

    #61814

    Purpose

    pUASp plasmid with attB for Drosophila transgenesis

    Depositor

    Frederique Peronnet

    Article

    Dardalhon-Cumenal et al PLoS Genet. 2018 Jul 11;14(7):e1007498.

    Type

    Empty backbone

    Tags

    6xMyc

    Expression

    Insect

    Promoter

    UASp

    Available Since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDmVasa

    Plasmid

    #165783

    Purpose

    Drosohphila vasa germline promoter and 5'UTR

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Vasa promoter
    

    Expression

    Insect

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pattB-DSCP-QF#7-hsp70

    Plasmid

    #46133

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7
    

    Expression

    Insect

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSLfa[Tc'hsp5'3'UTR]fa

    Plasmid

    #86450

    Purpose

    Heat shock-inducible promoter including hsp-UTRs, ubiquitous expression upon heat-shock of any inserted gene of interest in Tribolium; (Shuttle plasmid)

    Depositor

    Gregor Bucher

    Insert

    Tc'hsp5'3'UTR
    

    Expression

    Insect

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pACgp67B-Her2mP

    Plasmid

    #10793

    Depositor

    Judy Lieberman

    Article

    Song et al Nat Biotechnol. 2005 Jun . 23(6):709-17.

    Insert

    ErbB2 ScFv (ERBB2 Human)
    

    Tags

    6His and Truncated Protamine

    Expression

    Insect

    Available Since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFB-GTE-COI1

    Plasmid

    #29516

    Depositor

    Ning Zheng

    Article

    Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

    Insert

    COI1 (COI1 Mustard Weed)
    

    Tags

    GST and TEV cleavage site

    Expression

    Insect

    Mutation

    Note that the first AA in TEV site is R, not E

    Available Since

    May 26, 2011

    Availability

    Academic Institutions and Nonprofits only

33. 10xUAS-GFP-DD

    Plasmid

    #104989

    Purpose

    Expresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

    Depositor

    Jing-W Wang

    Article

    Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

    Insert

    TMP-inducible destabilized GFP (GFP-DD)
    

    Tags

    DD

    Expression

    Insect

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. psiTE(2)-Gal4

    Plasmid

    #220451

    Purpose

    Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-Gal4-CfaC
    

    Expression

    Insect

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. psiTE(0)-Gal4DBD

    Plasmid

    #220452

    Purpose

    Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-Gal4DBD-CfaC
    

    Expression

    Insect

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. psiTE(2)-Gal4DBD

    Plasmid

    #220454

    Purpose

    Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-Gal4DBD-CfaC
    

    Expression

    Insect

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. psiTE(1)-p65AD

    Plasmid

    #220456

    Purpose

    Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-p65AD-CfaC
    

    Expression

    Insect

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. psiTE(0)-Gal4

    Plasmid

    #220449

    Purpose

    Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-Gal4-CfaC
    

    Expression

    Insect

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. psiTE(2)-LexAGAD

    Plasmid

    #220460

    Purpose

    Split intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-LexAGAD-CfaC
    

    Expression

    Insect

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. psiTE(1)-Gal4DBD

    Plasmid

    #220453

    Purpose

    Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-Gal4DBD-CfaC
    

    Expression

    Insect

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge