4,958 results
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Plasmid#135558PurposeExpress human RABIF in mammalian cells, FLAG at N terminalDepositorAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only
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pFastBac-His6-Mps1-WT
Plasmid#114063Purposeprotein productionDepositorAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAc-GFP-dSUMO
Plasmid#85696Purposeexpression of GFP-SUMO1 in Drosophila cellsDepositorAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACE-GIRK4-S
Plasmid#172425PurposeExpression of full-length, human GIRK4 with C-terminal StrepTag IIDepositorAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10
Plasmid#218865PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8s
Tags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B-Agam_695
Plasmid#176668PurposeExpression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-UAS-NPRR_dTK
Plasmid#133244PurposeFluorescent reporter for neuropeptide release imagingDepositorInsertdrosophila tachykinin-fused codon-optimized GCaMP6s
ExpressionInsectPromoterhsp70Available SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
52 pLIB_3C_C_STREP
Plasmid#160300PurposeC-term 3C cleavable 2xStrepII tag for insect cell expressionDepositorTypeEmpty backboneTags2xStrepIIExpressionInsectPromoterpolyhydrinAvailable SinceNov. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393
Plasmid#216382PurposeBaculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11DepositorInsertTetraspanin-12 (TSPAN12 Human)
UseBaculovirusTags6xHis-3C-splitGFP11ExpressionInsectPromoterPolyhedrinAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393
Plasmid#216380PurposeBaculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10)DepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTags3C-splitGFP1to10opt-ETVV and HA signal sequence-F…ExpressionInsectPromoterPolyhedrinAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a
Plasmid#216385PurposeBaculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)DepositorInsertNorrin (NDP Human)
UseBaculovirusTagsGP64 signal sequence-MBP-3C and Rho1D4ExpressionInsectMutationC93A/C95A/C131APromoterPolyhedrinAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-3C-hNorrin(33-133)-1D4_pAcGP67a
Plasmid#216384PurposeBaculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218)DepositorInsertNorrin (NDP Human)
UseBaculovirusTagsGP64 signal sequence-MBP-3C and Rho1D4ExpressionInsectPromoterPolyhedrinAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac flag Bmi1
Plasmid#1967DepositorAvailable SinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTSTEP
Plasmid#72334PurposeTissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)DepositorInsertRRS-TagRFPT-p10-[Golic+] RRS-EGFP
UseCRISPRExpressionInsectAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab18
Plasmid#31740DepositorAvailable SinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMT Stat92E-GFP
Plasmid#126662PurposeInducible expression of Stat92E-GFP in S2 cellsDepositorInsertStat29E
ExpressionInsectAvailable SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK187
Plasmid#156423PurposeInsect cell expression of 3a-sfGFP, needs to be introduced into baculoviral bacmidDepositorInsertSARS-CoV-2 3a protein
TagssfGFP-HisExpressionInsectPromoterPolyhedrinAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3-nano
Plasmid#73616PurposeLight-induced nuclear export switch fusion to nano for microscopy in mammalian cells.DepositorInsertLINXa3-nano
TagsmCherryExpressionBacterial, Insect, and Mamm…Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACU
Plasmid#58373PurposeUAS vector to express gene of interest in DrosophilaDepositorTypeEmpty backboneExpressionInsectAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIE-hα2M-H6
Plasmid#128492PurposeExpresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.DepositorAvailable SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLfa-PUb-EGFP-3'UTR#5
Plasmid#176337PurposeThe synthetic 3'UTR#5 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoterDepositorInsert3'UTR#5
ExpressionInsectAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUAST cyto roGFP2-Orp1
Plasmid#64996Purposefor expression of cytosolic roGFP2-Orp1 with the Gal4 expression system in drosophilaDepositorAvailable SinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-BICDR1-GFP
Plasmid#111657PurposeC-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDR1-GFP (Bicdl1 Mouse)
Tags8xHis-tag, GFP, TEV site, and ZZExpressionInsectMutationOptimised for Sf9 expressionAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR-act5cB-GAL4QF
Plasmid#61309PurposeExpresses chimeric transactivator GAL4_DBD:QF2w_AD in DrosophilaDepositorInsertGAL4_DBD::QF2w_AD
ExpressionInsectMutationChanged EK on C-terminus of QF2 to KKKKAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC6-15XUAS-IVS-mCD8::GFP
Plasmid#26219DepositorInsertmCD8::GFP
ExpressionInsectAvailable SinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAC-QSco
Plasmid#46106DepositorInsertQSco
ExpressionInsectAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-TangoVG-lexA
Plasmid#138405PurposeRMCE vector for generating C-terminal Tango fusionDepositorInsertV2 tail-TEVcs(ENLYFQG)-LexAp65
ExpressionInsectAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFHMSP-LIC-N
Plasmid#26095PurposeDonor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expressionDepositorTypeEmpty backboneUseBaculovirus expressionTagsHis and TEV cleavage siteExpressionInsectPromoterpolyhedrinAvailable SinceSept. 15, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKSB- 3xP3-GFPsv40 GGGG AAGA
Plasmid#174531Purpose3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-GFP BsaI cassette
ExpressionInsectPromoter3xP3Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBac(3xP3-gTc’v; Tc-hsp68:Cre)
Plasmid#119063PurposeExpression of Cre recombinase in Tribolium castaneumDepositorInsertspBacL
Tc-hsp68 Promoter
Cre recombinase
Tc-hsp68 3'UTR
SV40polyA
Tc-vermilion
3xP3 TATA
pBacR
ExpressionInsectAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pFastBac (GST-preSci site-CD45)
Plasmid#177889PurposeBaculo expression of GST tag fused to human CD45 ICDDepositorInsertCD45 (aa 598-1304 only) (PTPRC Human)
TagsTEV-GST-PreScission cut siteExpressionInsectMutationL652PPromoterPolyhedrinAvailable SinceAug. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTIGER_mNeonGreen::3xFLAG::dCrk
Plasmid#131137PurposeUASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.DepositorAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2Bac.HA.p52
Plasmid#39219DepositorAvailable SinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFastBac GST-SAS6
Plasmid#80273Purposebaculovirus creation plasmid for GST-SAS6DepositorAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriExEKAR2G1
Plasmid#40174DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
TH1258-013_Jie_TAF15_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP
Plasmid#221893PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertTAF15 (TAF15 Human)
Tags6xHis-MBP-PreScission and TEV-mGFPExpressionInsectMutationwtPromoterPH promoterAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1259-014_Jie_EWSR1_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP
Plasmid#221894PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertEWSR1 (EWSR1 Human)
Tags6xHis-MBP-PreScission and TEV-mGFPExpressionInsectMutationwtPromoterPH promoterAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pUASp YFP Ras85D DN
Plasmid#52246PurposeDrosophila expression of YFP-Ras85D dominant negative mutantDepositorInsertRas85D (Ras85D Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS17N; DN mutationPromoterGAL4Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDmPolyUbiq
Plasmid#165786PurposeDrosophila Polyubiquitin promoter, strongest promoter in the libraryDepositorInsertPolyUbiq
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pattB-nsyb-M13::GAL4DBDo
Plasmid#64720Purposegenerating transgenic TRIC fliesDepositorInsertM13::GAL4DBDo
ExpressionInsectAvailable SinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab6
Plasmid#31734DepositorAvailable SinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-GL-DSCP
Plasmid#83338Purposefor Gateway cloning of enhancer elements upstream of the DSCP and GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsGFP::Luciferase(nls)ExpressionInsectPromoterDrosophila Synthetic Core PromoterAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
KCNK2
Plasmid#73257PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertKCNK2 (KCNK2 Human)
TagsTEV-His10-FLAGExpressionInsectMutationuserdefined:Full_Length 1411PromoterPolyhedrinAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
RAT
Plasmid#187416PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac(His10-SNAP-Csk)
Plasmid#177895PurposeBaculo expression of His10 tag fused to SNAPtag and human CskDepositorAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA mCherry SV40
Plasmid#176302PurposeExpresses mCherry mRNA from the Drosophila Metallothionein A promoterDepositorInsertmCherry
Tags3xFLAGExpressionInsectPromoterMtnAAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT EGFP SV40 pA
Plasmid#69911PurposeExpresses EGFP in DrosophilaDepositorInsertEGFP
ExpressionInsectPromoterMetallothionein Promoter (pMT)Available SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9S S730A
Plasmid#169946PurposePurification of catalytically-dead DPP9S from insect cellsDepositorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only