4,932 results

1. 52 pLIB_3C_C_STREP

   Plasmid

   #160300

   Purpose

   C-term 3C cleavable 2xStrepII tag for insect cell expression

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   2xStrepII

   Expression

   Insect

   Promoter

   polyhydrin

   Available Since

   Nov. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)

   Plasmid

   #55223

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Available Since

   July 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACU

   Plasmid

   #58373

   Purpose

   UAS vector to express gene of interest in Drosophila

   Depositor

   Yuh-Nung Jan, Chun Han

   Article

   Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUASp YFP Ras85D DN

   Plasmid

   #52246

   Purpose

   Drosophila expression of YFP-Ras85D dominant negative mutant

   Depositor

   Matthew Scott

   Insert

   Ras85D (Ras85D Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   S17N; DN mutation

   Promoter

   GAL4

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pMT Stat92E-GFP

   Plasmid

   #126662

   Purpose

   Inducible expression of Stat92E-GFP in S2 cells

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Stat29E
   

   Expression

   Insect

   Available Since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ac5-STABLE2-blast

   Plasmid

   #208034

   Purpose

   Enables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter.

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIE-hα2M-H6

   Plasmid

   #128492

   Purpose

   Expresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   A2M (A2M Human)
   

   Tags

   His tag (6x)

   Expression

   Insect

   Promoter

   IE1

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hy_pMtnA mCherry SV40

   Plasmid

   #176302

   Purpose

   Expresses mCherry mRNA from the Drosophila Metallothionein A promoter

   Depositor

   Jeremy Wilusz

   Article

   Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

   Insert

   mCherry
   

   Tags

   3xFLAG

   Expression

   Insect

   Promoter

   MtnA

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUAST cyto roGFP2-Orp1

   Plasmid

   #64996

   Purpose

   for expression of cytosolic roGFP2-Orp1 with the Gal4 expression system in drosophila

   Depositor

   Tobias Dick

   Article

   Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Tags

   roGFP2

   Expression

   Insect

   Available Since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTriEx-mCherry-LINXa3-nano

    Plasmid

    #73616

    Purpose

    Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa3-nano
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTSTEP

    Plasmid

    #72334

    Purpose

    Tissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)

    Depositor

    Avital Rodal

    Article

    Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

    Insert

    RRS-TagRFPT-p10-[Golic+] RRS-EGFP
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. His-ZZ-TEV-BICDR1-GFP

    Plasmid

    #111657

    Purpose

    C-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDR1-GFP (Bicdl1 Mouse)
    

    Tags

    8xHis-tag, GFP, TEV site, and ZZ

    Expression

    Insect

    Mutation

    Optimised for Sf9 expression

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFL-EGFP-3xFlag-Fml1

    Plasmid

    #193058

    Purpose

    Baculovirus expression of S.pombe Fml1 for recombinant protein production

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    Fml1 (fml1 Fission Yeast)
    

    Tags

    3xFlag

    Expression

    Insect

    Promoter

    polyhedron

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDmPolyUbiq

    Plasmid

    #165786

    Purpose

    Drosophila Polyubiquitin promoter, strongest promoter in the library

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    PolyUbiq
    

    Expression

    Insect

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACE-K2P10.1-StrepTagII

    Plasmid

    #191475

    Purpose

    Expresses K2P10.1 with C-terminal StrepTagII

    Depositor

    Arthur Laganowsky

    Article

    Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

    Insert

    K2P10.1 (KCNK10, TREK-2 Human)
    

    Expression

    Insect

    Mutation

    N144Q (uniprot P57789-1)

    Promoter

    polH

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGS_N_3xFLAG

    Plasmid

    #101654

    Purpose

    Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, KpnI linker, 3xFLAG tag
    

    Tags

    3xFLAG tag

    Expression

    Insect

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSBL_DK187

    Plasmid

    #156423

    Purpose

    Insect cell expression of 3a-sfGFP, needs to be introduced into baculoviral bacmid

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein
    

    Tags

    sfGFP-His

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJFRC6-15XUAS-IVS-mCD8::GFP

    Plasmid

    #26219

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    mCD8::GFP
    

    Expression

    Insect

    Available Since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF3BGX-TangoVG-lexA

    Plasmid

    #138405

    Purpose

    RMCE vector for generating C-terminal Tango fusion

    Depositor

    Shu Kondo

    Article

    Katow et al J Neurogenet. 2019 Mar - Jun;33(2):44-51

    Insert

    V2 tail-TEVcs(ENLYFQG)-LexAp65
    

    Expression

    Insect

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCaSpeR-act5cB-GAL4QF

    Plasmid

    #61309

    Purpose

    Expresses chimeric transactivator GAL4_DBD:QF2w_AD in Drosophila

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    GAL4_DBD::QF2w_AD
    

    Expression

    Insect

    Mutation

    Changed EK on C-terminus of QF2 to KKKK

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pRVV655

    Plasmid

    #119019

    Purpose

    Drives TP901-1 recombinase expression in the D. melanogaster germ line

    Depositor

    Richard Mann

    Article

    Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

    Insert

    TP901-1 recombinase
    

    Tags

    SV40 NLS

    Expression

    Insect

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pFastBac flag Bmi1

    Plasmid

    #1967

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    Bmi1 (Bmi1 Mouse)
    

    Tags

    flag

    Expression

    Insect

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pAC-QSco

    Plasmid

    #46106

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QSco
    

    Expression

    Insect

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. RAT

    Plasmid

    #187416

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    

    Expression

    Insect

    Promoter

    PH and p10

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFastBac(His10-SNAP-Csk)

    Plasmid

    #177895

    Purpose

    Baculo expression of His10 tag fused to SNAPtag and human Csk

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    Csk (CSK Human)
    

    Tags

    10xHis-TEV-TVMV-SNAP tag

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. OA-1050G (white)

    Plasmid

    #132420

    Purpose

    express arrays of gRNA targeting White under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    white gRNA array
    

    Expression

    Insect

    Promoter

    U6-3

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFastBac_Dual_GST-TEV-EGFP-TBK1

    Plasmid

    #187830

    Purpose

    Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Tags

    GST and TEV-mEGFP-GG Linker

    Expression

    Bacterial and Insect

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GFP-Rab6

    Plasmid

    #31734

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Rab6 (Rab6 Fly)
    

    Tags

    GFP

    Expression

    Insect

    Promoter

    Actin

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

29. p2Bac.HA.p52

    Plasmid

    #39219

    Depositor

    Aziz Sancar

    Insert

    p52 (GTF2H4 Human)
    

    Tags

    HA

    Expression

    Insect

    Promoter

    Pp10

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

30. pUASp YFP Ras64B DN

    Plasmid

    #52243

    Purpose

    Drosophila expression of YFP-Ras64B dominant negative mutant

    Depositor

    Matthew Scott

    Insert

    Ras64B (Ras64B Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    S19N; DN mutation

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

31. pUASp YFP Ras64B CA

    Plasmid

    #52244

    Purpose

    Drosophila expression of YFP-Ras64B constitutively active mutant

    Depositor

    Matthew Scott

    Insert

    Ras64B (Ras64B Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q63L; CA mutation

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pUASp YFP Ras64B WT

    Plasmid

    #52245

    Purpose

    Drosophila expression of YFP-Ras64B

    Depositor

    Matthew Scott

    Insert

    Ras64B (Ras64B Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pTIGER_mNeonGreen::3xFLAG::dCrk

    Plasmid

    #131137

    Purpose

    UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Tags

    3XFLAG and mNeonGreen

    Expression

    Insect

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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34. pFHMSP-LIC-N

    Plasmid

    #26095

    Purpose

    Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Baculovirus expression

    Tags

    His and TEV cleavage site

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 15, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

35. pattB-nsyb-M13::GAL4DBDo

    Plasmid

    #64720

    Purpose

    generating transgenic TRIC flies

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    M13::GAL4DBDo
    

    Expression

    Insect

    Available Since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. OA-1050E (Ubiq-CasRx)

    Plasmid

    #132416

    Purpose

    Express CasRx under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    CasRx
    

    Expression

    Insect

    Promoter

    Ubiq

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFastBac HTB DPP9S S730A

    Plasmid

    #169946

    Purpose

    Purification of catalytically-dead DPP9S from insect cells

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    S730A

    Promoter

    polyhedrin

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pOPINE-3C-HALO7

    Plasmid

    #41126

    Depositor

    Ray Owens

    Type

    Empty backbone

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G

    Plasmid

    #146392

    Purpose

    Insect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

    Insert

    DmeIF4G-trunc (eIF4G1 Fly)
    

    Expression

    Insect

    Mutation

    322-1666 truncated version of elF4G sequence with…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pUASt-GBP-Trbo

    Plasmid

    #179996

    Purpose

    Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASt version is ideal for expression in somatic tissues.

    Depositor

    Graydon Gonsalvez

    Article

    Baker et al Development. 2021 Nov 15;148(22). pii: 2

    Insert

    GBP nanobody
    

    Tags

    Trbo

    Expression

    Insect

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge